Research progress on aroma enhancement strategies for fruit wine

Shuo Ha , Tong Qu , Qingbo Zeng , Jiaojiao Liu , Jiaqi Zhang , Ming Chen , Hua Yang

Systems Microbiology and Biomanufacturing ›› 2025, Vol. 5 ›› Issue (4) : 1382 -1394.

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Systems Microbiology and Biomanufacturing ›› 2025, Vol. 5 ›› Issue (4) :1382 -1394. DOI: 10.1007/s43393-025-00395-8
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Research progress on aroma enhancement strategies for fruit wine
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Abstract

Fruit wine is an alcoholic beverage made from fruit through processes such as fermentation and ageing. It comes in a wide variety of types and styles. The flavour of fruit wine is a critical indicator of its quality. The aroma characteristics of fruit wine have been found to be significantly correlated with the composition and concentration of aromatic compounds present. Findings demonstrate that properly balanced aromatic substances contribute to better organoleptic qualities, enhanced product standards, and increased market appeal of fruit wines. Therefore, we should adopt appropriate methods to increase the content of aromatic compounds in fruit wine, thereby enhancing its popularity. This paper reviews several strategies for enhancing the aroma of fruit wine from various perspectives, including the selection of brewing materials, the regulation of microbial metabolism, the use of enzymes, the optimisation of the fermentation process, and the application of genetic engineering. It provides a reference for improving the flavour quality of fruit wine in the future.

Keywords

Fruit wine / Aroma enhancement strategies / fermentation microorganisms / fermentation process / Genetic engineering

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Shuo Ha, Tong Qu, Qingbo Zeng, Jiaojiao Liu, Jiaqi Zhang, Ming Chen, Hua Yang. Research progress on aroma enhancement strategies for fruit wine. Systems Microbiology and Biomanufacturing, 2025, 5(4): 1382-1394 DOI:10.1007/s43393-025-00395-8

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References

[1]

Álvarez R, Garces F, Louis EJ, Dequin S, Camarasa C. Beyond S. cerevisiae for winemaking: fermentation-related trait diversity in the genus Saccharomyces. Food Microbiol. 2023, 113 104270

[2]

Balmaseda A, Rozès N, Leal , Bordons A, Reguant C. Impact of changes in wine composition produced by non-Saccharomyces on malolactic fermentation. Int J Food Microbiol. 2021, 337 108954

[3]

Călugăr PC, Coldea TE, Pop CR, Stan L, Gal E, Ranga F, Hegheș SC, Mudura E. Mixed fermentations of yeasts and lactic acid bacteria as sustainable processes to enhance the chemical composition of cider made of Topaz and Red Topaz apple varieties. Agronomy. 2023, 13(10): 2485

[4]

Chen D, Liu SQ. Impact of simultaneous and sequential fermentation with Torulaspora delbrueckii and Saccharomyces cerevisiae on volatiles and non-volatiles of lychee wine. LWT Food Sci Technol. 2016, 65: 53-61

[5]

Chen X, Peng M, Wu D, Cai G, Yang H, Lu J. Physicochemical indicators and sensory quality analysis of kiwi wines fermented with different Saccharomyces cerevisiae. J Food Process Preserv. 2022, 46(11): e17132

[6]

Chen Y, Ding S, Zhang Y, Cui H, Chen X, Zhang J. Aroma enhancing effect of immobilized β-glucosidase in fruit wine and vinegar of Anli peel residues. Food Res Dev. 2023, 44(22): 30-37

[7]

Colomer MS, Chailyan A, Fennessy RT, Olsson KF, Johnsen L, Solodovnikova N, Forster J. Assessing population diversity of Brettanomyces yeast species and identification of strains for brewing applications. Front Microbiol. 2020, 11: 637

[8]

Csutoras Cs, Bakos-Barczi N, Burkus B. Medium chain fatty acids and fatty acid esters as potential markers of alcoholic fermentation of white wines. Acta Aliment. 2022, 51(1): 33-42

[9]

De Ovalle S, Brena B, González-Pombo P. Influence of beta glucosidases from native yeast on the aroma of Muscat and Tannat wines. Food Chem. 2021, 346 128899

[10]

Du Plessis H, Du Toit M, Nieuwoudt H, Van Der Rijst M, Hoff J, Jolly N. Modulation of wine flavor using Hanseniaspora uvarum in combination with different Saccharomyces cerevisiae, lactic acid bacteria strains and malolactic fermentation strategies. Fermentation. 2019, 5(3): 64

[11]

Escudero A, Arias I, Lacau B, Astraín J, Barón C, Fernandez-Zurbano P, Ferreira V. Effects of vineyard ‘potential’ and grape maturation on the aroma-volatile profile of Grenache wines. OENO One, 2019;53(4).

[12]

Han X, Peng Q, Yang H, Hu B, Shen C, Tian R. Influence of different carbohydrate sources on physicochemical properties and metabolites of fermented greengage (Prunus mume) wines. LWT. 2020, 121 108929

[13]

Hao N, Liu J, Wang F, Cao J, Chen Q, He L, Guan X, Liu S, Shi K. Malolactic fermentation performance of indigenous Oenococcus oeni strains from Shaanxi wine region (China) and their mutants on Pinot Noir and Chardonnay wines. LWT. 2023, 185 115170

[14]

Herrero Ó, Ramón D, Orejas M. Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine. Metab Eng. 2008, 10(2): 78-86

[15]

Hu L, Liu R, Wang X, Zhang X. Improving the sensory quality of citrus wine through co-fermentation with selected non-Saccharomyces yeast strains and Saccharomyces cerevisiae. Microorganisms. 2020, 8(3): 323

[16]

Hu L, Chen X, Lin R, Xu T, Xiong D, Li L, Zhao Z. Quality improvement in apple ciders during simultaneous co-fermentation through triple mixed-cultures of Saccharomyces cerevisiae, Pichia kudriavzevii, and Lactiplantibacillus plantarum. Foods. 2023, 12(3): 655

[17]

Huang M, Liu X, Li X, Sheng X, Li T, Tang W, Yu Z, Wang Y. Effect of Hanseniaspora uvarumSaccharomyces cerevisiae mixed fermentation on aroma characteristics of Rosa roxburghii Tratt, blueberry, and plum wines. Molecules. 2022, 27(22): 8097

[18]

Jiang X, Lu Y, Liu SQ. Effects of different yeasts on physicochemical and oenological properties of red dragon fruit wine fermented with Saccharomyces cerevisiae, Torulaspora delbrueckii and Lachancea thermotolerans. Microorganisms. 2020, 8(3)) 315

[19]

Jiang X, Lu Y, Liu SQ. Effects of pectinase treatment on the physicochemical and oenological properties of red dragon fruit wine fermented with Torulaspora delbrueckii. LWT. 2020, 132 109929

[20]

Kim B-H, Park SK. Volatile aroma and sensory analysis of black raspberry wines fermented by different yeast strains: volatiles in Korean black raspberry wine. J Inst Brew. 2015, 121(187-94

[21]

Labrador Fernández L, Pérez-Porras P, Díaz-Maroto MC, Gómez-Plaza E, Pérez-Coello MS, Bautista-Ortín AB. The technology of high-power ultrasound and its effect on the color and aroma of rosé wines. J Sci Food Agric. 2023, 103(13): 6616-6624

[22]

Lai Y-T, Hsieh C-W, Lo Y-C, Liou B-K, Lin H-W, Hou C-Y, Cheng K-C. Isolation and identification of aroma-producing non-Saccharomyces yeast strains and the enological characteristic comparison in wine making. LWT. 2022, 154 112653

[23]

Lerno LA, Panprivech S, Ponangi R, Brenneman CA, Block DE, Oberholster A. Impact of cold soak duration on Cabernet Sauvignon fermentation and phenolic composition. J Sci Food Agric. 2019, 99(2): 805-815

[24]

Li N, Wang Q-Q, Xu Y-H, Li A-H, Tao Y-S. Increased glycosidase activities improved the production of wine varietal odorants in mixed fermentation of P. fermentans and high antagonistic S. cerevisiae. Food Chem. 2020, 332 127426

[25]

Liu P-T, Lu L, Duan C-Q, Yan G-L. The contribution of indigenous non-Saccharomyces wine yeast to improved aromatic quality of Cabernet Sauvignon wines by spontaneous fermentation. LWT Food Sci Technol. 2016, 71: 356-363

[26]

Liu M, Yang K, Qi Y, Zhang J, Fan M, Wei X. Fermentation temperature and the phenolic and aroma profile of persimmon wine. J Inst Brew. 2018, 124(3): 269-275

[27]

Liu X, Xu S, Wang M, Wang L, Liu J. Effect of mixed fermentation with Pichia fermentans, Hanseniaspora uvarum, and Wickeramomyces anomala on the quality of fig (Ficus carica L.) wines. J Food Process Preserv. 2021, 45(2): e15169

[28]

Liu C, Li M, Ren T, Wang J, Niu C, Zheng F, Li Q. Effect of Saccharomyces cerevisiae and non-Saccharomyces strains on alcoholic fermentation behavior and aroma profile of yellow-fleshed peach wine. LWT. 2022, 155 112993

[29]

Liu J, Wang Q, Weng L, Zou L, Jiang H, Qiu J, Fu J. Analysis of sucrose addition on the physicochemical properties of blueberry wine in the main fermentation. Front Nutr. 2023, 9: 1092696

[30]

Lola D, Kalloniati C, Tzamourani A, Paramithiotis S, Dimopoulou M, Flemetakis E, Kotseridis Y. The use of autochthonous Saccharomyces cerevisiae strains as a strategy to enhance aroma variability and typicity of Savatiano wines; RNAseq-based transcriptome comparison of indigenous strains under winemaking conditions. Int J Food Microbiol. 2025

[31]

Maluleke E, Lekganyane MA, Moganedi KLM. Microbial diversity of Marula wine during spontaneous fermentation. Fermentation. 2023, 9(10): 862

[32]

Marcon ÂR, Schwarz LV, Dutra SV, Delamare APL, Gottardi F, Parpinello GP, Echeverrigaray S. Chemical composition and sensory evaluation of wines produced with different Moscato varieties. BIO Web Conf. 2019, 12: 02033

[33]

Markkinen N, Pariyani R, Jokioja J, Kortesniemi M, Laaksonen O, Yang B. NMR-based metabolomics approach on optimization of malolactic fermentation of sea buckthorn juice with Lactiplantibacillus plantarum. Food Chem. 2022, 366 130630

[34]

Martins V, López R, Teixeira A, Gerós H. Combined omics expose microbial niches of fungi and bacteria correlating with wine volatile profiles in Douro wine region. LWT. 2024, 193 115769

[35]

Massera A, Assof M, Sari S, Ciklic I, Mercado L, Jofré V, Combina M. Effect of low temperature fermentation on the yeast-derived volatile aroma composition and sensory profile in Merlot wines. LWT. 2021, 142 111069

[36]

Miao Y, Li J, Wang S, Li C, Wang Y. Research progress of key enzymes in terpene biosynthesis. China Biotechnol. 2021, 41(6): 60-70

[37]

Miljic U, Puskas V. Influence of fermentation conditions on production of plum (Prunus domestica L.) wine: a response surface methodology approach. Hem Ind. 2014, 68(2): 199-206

[38]

Olejar KJ, Fedrizzi B, Kilmartin PA. Influence of harvesting technique and maceration process on aroma and phenolic attributes of Sauvignon blanc wine. Food Chem. 2015, 183: 181-189

[39]

Park S-H, Kim S, Hahn J-S. Metabolic engineering of Saccharomyces cerevisiae for the production of isobutanol and 3-methyl-1-butanol. Appl Microbiol Biotechnol. 2014, 98(21): 9139-9147

[40]

Patel V, Tripathi AD, Adhikari KS, Srivastava A. Screening of physicochemical and functional attributes of fermented beverage (wine) produced from local mango (Mangifera indica) varieties of Uttar Pradesh using novel Saccharomyces strain. J Food Sci Technol. 2021, 58(6): 2206-2215

[41]

Peng C-T, Wen Y, Tao Y-S, Lan Y-Y. Modulating the formation of Meili wine aroma by prefermentative freezing process. J Agric Food Chem. 2013, 61(7): 1542-1553

[42]

Peng B, Li F, Cui L, Guo Y. Effects of fermentation temperature on key aroma compounds and sensory properties of apple wine. J Food Sci. 2015, 80(12): S2937-S2943

[43]

Peng W, Meng D, Yue T, Wang Z, Gao Z. Effect of the apple cultivar on cloudy apple juice fermented by a mixture of Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus fermentum. Food Chem. 2021, 340 127922

[44]

Picard M, Tempere S, De Revel G, Marchand S. A sensory study of the ageing bouquet of red Bordeaux wines: a three-step approach for exploring a complex olfactory concept. Food Qual Prefer. 2015, 42: 110-122

[45]

Qin T, Liao J, Zheng Y, Zhang W, Zhang X. Oenological characteristics of four non-Saccharomyces yeast strains with β-glycosidase activity. Front Microbiol. 2021, 12 626920

[46]

Qin L, Huang M, Ma Y, Zhang D, Cui Y, Kang W. Effects of two Saccharomyces cerevisiae strains on physicochemical and oenological properties of Aranèle white wine. Food Biosci. 2023, 52 102495

[47]

Reddy LVA, Reddy OVS. Effect of fermentation conditions on yeast growth and volatile composition of wine produced from mango (Mangifera indica L.) fruit juice. Food Bioprod Process. 2011, 89(4): 487-491

[48]

Reynolds A, Cliff M, Girard B, Kopp TG. Influence of fermentation temperature on composition and sensory properties of Semillon and Shiraz wines. Am J Enol Vitic. 2001, 52(3): 235-240

[49]

Rollero S, Roberts S, Bauer FF, Divol B. Agitation impacts fermentation performance as well as carbon and nitrogen metabolism in Saccharomyces cerevisiae under winemaking conditions: influence of agitation on yeast metabolism. Aust J Grape Wine Res. 2018, 24(3): 360-367

[50]

Scutarașu EC, Luchian CE, Vlase L, Nagy K, Colibaba LC, Trinca LC, Cotea VV. Influence evaluation of enzyme treatments on aroma profile of white wines. Agronomy. 2022, 12(11): 2897

[51]

Shen L, Nishimura Y, Matsuda F, Ishii J, Kondo A. Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose. J Biosci Bioeng. 2016, 122(1): 34-39

[52]

Shumin Y, Mengqi Z, Mengqi Z, Qike Y, Yuxi L. Analysis of microbial diversity in Muscat Hamburg wine and Shine-Muscat wine. Emirates J Food Agric. 2023, 35: 189-196

[53]

Sun W, Chen X, Feng S, Bi P, Han J, Li S, Guo J. Effect of sequential fermentation with indigenous non-Saccharomyces cerevisiae combinations and Saccharomyces cerevisiae on the chemical composition and aroma compounds evolution of kiwifruit wine. Food Chem. 2024, 460 140758

[54]

Snyman C. Impact of the protease-secreting yeast Metschnikowia pulcherrima IWBT Y1123 on wine properties and response of protease production to nitrogen sources. 2019. [Master Thesis, Stellenbosch University].

[55]

Swiegers JH, Bartowsky EJ, Henschke PA, Pretorius IS. Yeast and bacterial modulation of wine aroma and flavour. Aust J Grape Wine Res. 2005, 11(2): 139-173

[56]

Taniasuri F, Lee P-R, Liu S-Q. Induction of simultaneous and sequential malolactic fermentation in durian wine. Int J Food Microbiol. 2016, 230: 1-9

[57]

Tian M-B, Hu R-Q, Liu Z-L, Shi N, Lu H-C, Duan C-Q, Wang J, Sun Y-F, Kong Q-S, He F. The pH adjustment of Vitis amurensis dry red wine revealed the evolution of organic acids, volatomics, and sensory quality during winemaking. Food Chem. 2024, 436 137730

[58]

Trindade De Carvalho B, Holt S, Souffriau B, Lopes Brandão R, Foulquié-Moreno MR, Thevelein JM. Identification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast. mBio. 2017;8(6):e01173–17.

[59]

Tufariello M, Rizzuti A, Palombi L, Ragone R, Capozzi V, Gallo V, Mastrorilli P, Grieco F. Non-targeted metabolomic approach as a tool to evaluate the chemical profile of sparkling wines fermented with autochthonous yeast strains. Food Control. 2021, 126 108099

[60]

Van Wyk N, Grossmann M, Wendland J, Von Wallbrunn C, Pretorius IS. The whiff of wine yeast innovation: strategies for enhancing aroma production by yeast during wine fermentation. J Agric Food Chem. 2019, 67(49): 13496-13505

[61]

Van Wyk N, Kroukamp H, Espinosa MI, Von Wallbrunn C, Wendland J, Pretorius IS. Blending wine yeast phenotypes with the aid of CRISPR DNA editing technologies. Int J Food Microbiol. 2020, 324 108615

[62]

Wang X-C, Li A-H, Dizy M, Ullah N, Sun W-X, Tao Y-S. Evaluation of aroma enhancement for “Ecolly” dry white wines by mixed inoculation of selected Rhodotorula mucilaginosa and Saccharomyces cerevisiae. Food Chem. 2017, 228: 550-559

[63]

Wang S, Li Y, Ren J, Gu P, Li T, Wu Y, Zhang B, Zhu B. Comparison on evolution of volatile compounds and aroma attributes in different pH-adjusted fermented bog bilberry syrup wines during bottle-aging period. Food Biosci. 2018, 22: 121-128

[64]

Wang Z, Chen K, Liu C, Ma L, Li J. Effects of glycosidase on glycoside-bound aroma compounds in grape and cherry juice. J Food Sci Technol. 2023, 60(2): 761-771

[65]

Wei J, Li Y, Liu Y, Liu S, Yang X, Wang X. Process optimization for production of persimmon wine with lower methanol. Foods. 2024, 13(5): 748

[66]

Whitener MEB, Stanstrup J, Carlin S, Divol B, Du Toit M, Vrhovsek U. Effect of non-Saccharomyces yeasts on the volatile chemical profile of Shiraz wine: Shiraz wines fermented by non-Saccharomyces yeasts. Aust J Grape Wine Res. 2017, 23(2): 179-192

[67]

Yang H, Sun J, Tian T, Gu H, Li X, Cai G, Lu J. Physicochemical characterization and quality of Dangshan pear wines fermented with different Saccharomyces cerevisiae. J Food Biochem. 2019, 43(8) e12891

[68]

Yang H, Cai G, Lu J, Gómez Plaza E. The production and application of enzymes related to the quality of fruit wine. Crit Rev Food Sci Nutr. 2021, 61(10): 1605-1615

[69]

Yang X, Zhao F, Yang L, Li J, Zhu X. Enhancement of the aroma in low-alcohol apple-blended pear wine mixed fermented with Saccharomyces cerevisiae and non-Saccharomyces yeasts. LWT. 2022, 155 112994

[70]

Ye X, Zhang X, Hao L, Lin Q, Bao Y. Effects of different fermentation methods on the quality and microbial diversity of passion fruit wine. Fermentation. 2023, 9(5): 439

[71]

Yin X-L, Gong L-J, Zhong W, Li E-H. Effects of mixed fermentation with Torulaspora delbrueckii and Saccharomyces cerevisiae on aroma profile of kiwifruit wine. Food Science. 2021, 42(22): 216-223

[72]

Zhang Y, Sun X, Lan W, Zhang X, Liu W, Wang S. Dynamic analysis on fungal community of naturally fermented strawberry wine. J Fuyang Teachers College (Nat Sci Edn), 2018;35(2):21–26, 42.

[73]

Zhang W-W, Weng P-F, Wu Z-F. Fermentation efficiency and flavor profile analysis of bayberry wine by mixed culture of Issatchenkia orientalis and Saccharomyces cerevisiae. Food Sci. 2019, 40(18): 144-151

[74]

Zhang X, Wu Y, Chen X, Chen X, Yang H, Lu J, Wu D. Screening of a Saccharomyces cerevisiae strain with high esterase production by ARTP mutagenesis for jackfruit wine brewing. Food Ferment Ind. 2022, 48(22): 110-118

[75]

Zhang J, Wang X, Zhang F, Sun Y. Influence of an indigenous yeast, CECA, from the Ningxia wine region of China, on the yeast community dynamics and aroma of Cabernet Sauvignon wines. LWT. 2023, 189 115506

[76]

Zheng S-D, Chen G, Que F-X, Wan L, Deng S-H, Jian S-P. Effects of mixed fermentation with Kloeckera apiculata and Saccharomyces cerevisiae on flavor compounds of whole-fruit navel orange wine. Food Ferment Ind. 2019, 45(12): 101-108

[77]

Zhong Q, Chen R, Zhang M, Chen W, Chen H, Chen W. Effect of the mixed inoculation of lactic acid bacteria and non-Saccharomyces on the quality and flavor enhancement of fermented mango juice. Fermentation. 2023, 9(6): 563

[78]

Zhu L-X, Wang H, Han P, Lan Y-B. Identification of dominant functional microbes that contribute to the characteristic aroma of Msalais, traditional wine fermented from boiled local grape juice in China. Food Chem: X. 2023, 19: 100778

[79]

Zilelidou EA, Nisiotou A. Understanding wine through yeast interactions. Microorganisms. 2021, 9(8)) 1620

Funding

2024 Liaoning Provincial University Basic Scientific Research Fund Project(LJ212410152025)

Project of Liaoning Provincial Natural Science Foundation(2024-MS-171)

Dalian Polytechnic University to Introduce Talents Research Start-up Funding Project(LJBKY2025058)

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Jiangnan University

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