Simple and robust method for the quantification of nine organic acids in beer using 3-chloropropyl bonded C18 materials with HPLC-UV analysis

Abstract

A simple and robust reversed-phase high performance liquid chromatography procedure was developed with only an aqueous H₃PO₄ solution (pH 2.1) as the mobile phase for the simultaneous determination of nine organic acids (oxalic, formic, malic, lactic, acetic, citric, succinic, fumaric, and gallic acids) in beer based on a polar-copolymerized 3-chloropropyl C18 column. The proposed method was validated according to linearity, limits of detection, accuracy, precision, repeatability, and reproducibility. Quantifications of the nine organic acids in 12 commercial Chinese beer samples from five different brands were conducted only after degassing and filtration of the samples. In all beer samples, the most abundant organic acid was succinic acid; its concentration was in the range of 907.99–3557.22 mg/L. This was followed by malic acid (170.39–936.78 mg/L). Principal component analysis was used to compare the different brand of beers according to organic acid concentration, and results may provide a reference for the evaluation of beer quality and taste.

Keywords

Organic acids / Beer / High performance liquid chromatography / Principal component analysis / Quantification

References

[1] LinR, PengZ, LiW, ChuJ, LuJ, XieG, WuD. Evaluation of polyphenols content and antioxidant ability of commercial herbal beer. Syst Microbiol Biomanuf, 2023, 4177-85.

[2] AndersonHE, SantosIC, HildenbrandZL, SchugAK. A review of the analytical methods used for beer ingredient and finished product analysis and quality control. Anal Chim Acta, 2019, 1085: 1-20.

[3] RodriguesJ, ErnyGL, BarrosAS, et al. . Quantification of organic acids in beer by nuclear magnetic resonance (NMR)-based methods. Anal Chim Acta, 2010, 6742166-5.

[4] PaiTV, SawantSY, GhatakAA, ChaturvediPA, GupteAM, DesaiNS. Characterization of Indian beers: chemical composition and antioxidant potential. J Food Sci Tech, 2015, 52: 1414-23.

[5] LiH, LiuF. Changes in organic acids during beer fermentation. J Am Soc Brew Chem, 2015, 73: 275-9.

[6] WhitingGC. Organic acid metabolism of yeasts during fermentation of alcoholic beverages— A review. J I Brew, 1976, 82284-92.

[7] MatoI, Suarez-LuqueS, HuidobroJF. A review of the analytical methods to determine organic acids in grape juices and wines. Food Res Int, 2005, 38101175-88.

[8] CostaMP, ConteCA. Chromatographic methods for the determination of carbohydrates and organic acids in foods of animal origin. Compr Rev Food Sci F, 2015, 145586-600.

[9] RoblesA, FabjanowiczM, ChmielT, Płotka-WasylkaJ. Determination and identification of organic acids in wine samples. Problems and challenges. Trac-Trend Anal Chem, 2019, 120: 115630.

[10] QiuX, ZhangY, ZhouY, LiG, FengX. Progress in pretreatment and analysis of organic acids: an update since 2010. Food Chem, 2021, 360: 129977.

[11] ParkJM, ShinJA, LeeJH, LeeKT. Development of a quantitative method for organic acid in wine and beer using high performance liquid chromatography. Food Sci Biotechnol, 2017, 26: 349-55.

[12] RomeroEG, MunozGS, AlvarezP, CabezudoMD. Determination of organic acids in grape musts, wines and vinegars by high-performance liquid chromatography. J Chromatogr A, 1993, 6551111-7.

[13] MontanariL, PerrettiG, NatellaF, GuidiA, FantozziP. Organic and phenolic acids in beer. LWT-Food Sci Technol, 1999, 328535-39.

[14] Perez-RuizT, Martínez-LozanoC, TomasV, MartínJ. High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection. J Chromatogr A, 2004, 10261–257-64.

[15] DasAJ, KhawasP, MiyajiT, DekaSC. HPLC and GC-MS analyses of organic acids, carbohydrates, amino acids and volatile aromatic compounds in some varieties of rice beer from northeast India. J I Brew, 2014, 1203244-52.

[16] ManciniF, MiniatiE, MontanariL. Determination of organic acid anions in Italian beers by a new HPLC method. Ital J Food Sci, 2000, 124443-50

[17] PetterssonSW, PerssonBS, NystromM. General method allowing the use of 100% aqueous loading conditions in reversed-phase liquid chromatography. J Chromatogr B, 2004, 8031159-65.

[18] Ivanova-PetropulosV, PetruevaD, MitrevS. Rapid and simple method for determination of target organic acids in wine using HPLC-DAD analysis. Food Ana Method, 2020, 13: 1078-87.

[19] GuoZ, WangC, LiangT, LiangX. Polar-copolymerized approach based on horizontal polymerization on silica surface for preparation of polar-modified stationary phases. J Chromatogr A, 2010, 1217274555-60.

[20] WangH, ChenL, TangX, JiaY, LiG, SunX, WenA. Synthesis and characterization of novel polar-embedded silica stationary phases for use in reversed-phase high-performance liquid chromatography. J Chromatogr A, 2013, 12711153-62.

[21] LiX, HuangR, LiuY, JinH, WanH, ZhaoJ, ZhaoW, LiangX. Efficient purification of low molecular weight nitrogen polar compounds from the skin of Bufo bufo gargarizans Cantor by reversed-phase high performance liquid chromatography with a polar-copolymerized C18 stationary phase. Anal Methods-UK, 2014, 6: 5183-90.

[22] FuD, ZhangX, ZhangH, FuQ, JinY, YanJ, LiX. Simple and efficient preparation of high-purity trehalulose from the waste syrup of isomaltulose production using solid-phase extraction followed by hydrophilic interaction chromatography. J Sep Sci, 2021, 44122334-42.

[23] ZengJ, GuoZ, XiaoY, WangC, LiangX. Purification of polar compounds from Radix isatidis using conventional C18 column coupled with polar-copolymerized C18 column. J Sep Sci, 2010, 33213341-6.

[24] LiZ, JiangD, DaiY, DaiZ, JinY, FuQ, LiangX. Isolation of three polyoxins by reversed-phase liquid chromatography with pure aqueous mobile phase. J Sep Sci, 2021, 44102020-28.

[25] ZeppaG, ConternoL, GerbiV. Determination of organic acids, sugars, diacetyl, and acetoin in cheese by high-performance liquid chromatography. J Agric Food Chem, 2001, 4962722-6.

[26] ZotouA, LoukouZ, KaravaO. Method development for the determination of seven organic acids in wines by reversed-phase high performance liquid chromatography. Chromatographia, 2004, 60: 39-44.

Funding

Foundation of Liaoning Province Education Administration(LJKZ0516)

RIGHTS & PERMISSIONS

Jiangnan University