Leveraging CRISPR/Cas9 in notable bacteria for the production of industrially valuable compounds

Abstract

Scientists are traditionally relied on bacteria to find and generate new natural chemicals. Gene editing research to identify, biosynthesize, and metabolically design natural chemicals is popular. The conventional genome editing relies on host or imported protein recombination. Microorganism’s diverse genetic history makes universal platforms difficult. The genetic variety renders experiments time consuming and useless. The CRISPR/Cas9 gene editing technique offers more functional diversity because to its diverse targeting capabilities, surpassing conventional approaches constrained by sequence homology or site restrictions. This enhances productivity, streamlines trials, and propels the research of natural goods. The CRISPR/Cas9 genetic editing technology may surpass sequence or location related constraints of earlier gene editing methods due to its targeting versatility. This methodology aids researchers investigating natural goods by optimizing and enhancing experimental techniques. This article provides an overview of the CRISPR/CRISPR-associated (Cas) mechanism, a transformative genome engineering technique in molecular biology. This paper aims to highlight and analyze the applications of CRISPR/Cas, particularly CRISPR/SpCas9, in genome editing for the identification of natural products. The creatures discussed embrace bacteria such as, Streptomyces, Bacillus, Clostridium, Corynebacterium, Myxobacteria and Escherichia. In a nutshell we will examine the potential benefits of using CRISPR/Cas in the discovery of natural products.

Keywords

Bacteria / CRISPR/Cas9 / Genome editing / Natural compounds / Metabolites / Biological Sciences / Genetics

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