Cloning, expression, and characterization of thermostable α-amylase of a thermophilic bacterium isolated from hot-spring of Sikkim and sustainable immobilization on coconut coir

Abstract

•	• The recombinant α-amylase AmyK2 is highly thermotolerant, stable in a wide range of pH and Ca+ 2 independent enzyme.
•	• The enzyme having low Km for starch; therefore, it exhibits high affinity for any starchy substrate.
•	• The enzyme was successfully immobilized at its native state with coconut coir and effective after successive reuse.

Keywords

Thermostable / Bacillus subtilis k2cm / Recombinant α-amylase / Immobilization / Specific activity

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