The combined inoculation of yeast and lactic acid bacteria (LAB) is a promising approach to enhance microbial metabolism and the quality of fermented food products. In this study, we investigated the effects of simultaneous and sequential inoculation of LAB and yeast on coffee fermentation. Fermentations were conducted using single and combined protocols with P. fermentans YC5.2 and P. pentosaceus LPBF07. A temporal analysis utilizing Illumina high-throughput rRNA Gene Sequencing revealed a synergistic interaction between the two microbial groups. This positive synergy led to increased consumption of coffee pulp sugar and the production of metabolites, surpassing the results observed in single cultures and the spontaneous process. Furthermore, the combined inoculation processes demonstrated a more significant role in suppressing wild microbiota compared to single cultures. Notably, the sequential process emerged as particularly effective in promoting a more intricate aroma profile and elevated sensorial score, attributed to the formation of distinctive compounds such as benzeneacetaldehyde, 1-hexanol, 2-heptanol, benzyl alcohol and phenylethyl alcohol. These results suggest that implementing a sequential inoculation process could enable coffee farmers to standardize on-farm processing and produce high-value-added products.
| [1] |
Food Outlook – Biannual report on global food markets; FAO, 2023;
|
| [2] |
de Melo Pereira GV, de CarvalhoNeto DP, MagalhãesJúnior AI, Vásquez ZS, Medeiros ABP, Vandenberghe LPS, Soccol CR. Exploring the impacts of postharvest processing on the aroma formation of coffee beans – a review. Food Chem. 2019, 272: 441-452
|
| [3] |
de Oliveira Junqueira AC, de Melo Pereira GV, Coral Medina JD, Alvear MCR, Rosero R, de CarvalhoNeto DP, Enríquez HG, Soccol CR. First description of bacterial and fungal communities in colombian coffee beans fermentation analysed using illumina-based amplicon sequencing. Sci Rep. 2019
|
| [4] |
Elhalis H, Cox J, Zhao J. Coffee fermentation: expedition from traditional to controlled process and perspectives for industrialization. Appl Food Res. 2023, 3 100253
|
| [5] |
de Melo Pereira GV, Soccol VT, Pandey A, Medeiros ABP, Andrade Lara JMR, Gollo AL, Soccol CR. Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. Int J Food Microbiol. 2014, 188: 60-66
|
| [6] |
de Melo Pereira GV, Neto E, Soccol VT, Medeiros ABP, Woiciechowski AL, Soccol CR. Conducting starter culture-controlled fermentations of coffee beans during on-farm wet processing: growth, metabolic analyses and sensorial effects. Food Res Int. 2015, 75: 348-356
|
| [7] |
de Pereira GVM, de CarvalhoNeto DP, Medeiros ABP, Soccol VT, Neto E, Woiciechowski AL, Soccol CR. Potential of lactic acid bacteria to improve the fermentation and quality of coffee during on-farm processing. Int J Food Sci Technol. 2016, 51: 1689-1695
|
| [8] |
de Junqueira ACO, Vinícius de Melo Pereira G, Viesser JA, de CarvalhoNeto DP, Querne LBP, Soccol CR. Isolation and selection of fructose-consuming lactic acid bacteria associated with coffee bean fermentation. Food Biotechnol. 2022, 2022(36): 58-75
|
| [9] |
da Silva Vale A, Balla G, Rodrigues LRS, de CarvalhoNeto DP, Soccol CR, de Melo Pereira GV. Understanding the effects of self-induced anaerobic fermentation on coffee beans quality: microbiological, metabolic, and sensory studies2023Foods.
|
| [10] |
da Silva Vale A, de Melo Pereira GV, de Carvalho Neto DP, Sorto RD, Goés-Neto A, Kato R, Soccol CR. Facility-specific ‘house’ microbiome ensures the maintenance of functional microbial communities into coffee beans fermentation: implications for source tracking. Environ Microbiol Rep. 2021, 13: 470-481
|
| [11] |
Pregolini VB, de Melo Pereira GV, da Silva Vale A, de CarvalhoNeto DP, Soccol CR. Influence of environmental microbiota on the activity and metabolism of starter cultures used in coffee beans fermentation. Fermentation. 2021
|
| [12] |
De Bruyn F, Zhang SJ, Pothakos V, Torres J, Lambot C, Moroni AV, Callanan M, Sybesma W, Weckx S, De Vuyst L. Exploring the impacts of postharvest processing on the microbiota and metabolite profiles during green coffee bean production. Appl Environ Microbiol. 2017
|
| [13] |
de CarvalhoNeto DP, de Melo Pereira GV, de Carvalho JC, Soccol VT, Soccol CR. High-throughput RRNA gene sequencing reveals high and complex bacterial diversity associated with brazilian coffee beans fermentation. Food Technol Biotechnol. 2018
|
| [14] |
de Melo Pereira GV, de Carvalho Neto DP, Maske BL, De Dea Lindner J, Vale AS, Favero GR, Viesser J, de Carvalho JC, Góes-Neto A, Soccol CR. An updated review on bacterial community composition of traditional fermented milk products: what next-generation sequencing has revealed so far?. Crit Rev Food Sci Nutr. 2022, 62: 1870-1889
|
| [15] |
Lopes MR, Santos ARO, Moreira JD, Santa-Brígida R, Martins MB, Pinto FO, Valente P, Morais PB, Jacques N, Grondin C, et al. . Kurtzmaniella Hittingeri f.a Sp. Nov., isolated from rotting wood and fruits, and transfer of three Candida species to the genus Kurtzmaniella as new combinations. Int J Syst Evol Microbiol. 2019, 69: 1504-1508
|
| [16] |
Lachance MA, Starmer WT. Kurtzmaniella Gen. Nov. and description of the heterothallic, haplontic yeast species kurtzmaniella cleridarum Sp. Nov., the teleomorph of Candidacleridarum. Int J Syst Evol Microbiol. 2008, 58: 520-524
|
| [17] |
Arrey G, Li G, Murphy R, Guimaraes L, Alizadeh S, Poulsen M, Regenberg B. Isolation, Characterization, and Genome Assembly of BarnettozymaBotsteinii Sp. Nov. and novel strains of KurtzmaniellaQuercitrusa isolated from the intestinal tract of the termite MacrotermesBellicosus. G3.. 2023
|
| [18] |
da Silva Vale A, de Melo Pereira GV, de CarvalhoNeto DP, Rodrigues C, Pagnoncelli MGB, Soccol CR. Effect of co-inoculation with pichia fermentans and pediococcus acidilactici on metabolite produced during fermentation and volatile composition of coffee beans. Fermentation. 2019
|
| [19] |
Peetermans A, Foulquié-Moreno MR, Thevelein JM. Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomycescerevisiae. Microbial Cell. 2021, 8: 111-130
|
| [20] |
Muynarsk ESM, de Melo Pereira GV, Mesa D, Thomaz-Soccol V, Carvalho JC, Pagnoncelli MGB, Soccol CR. Draft genome sequence of pediococcus acidilactici strain LPBC161 isolated from mature coffee cherries during natural fermentation. Microbiol Resour Announc. 2019
|
| [21] |
Manoochehri H, Hosseini NF, Saidijam M, Taheri M, Rezaee H, Nouri F. A Review on invertase: its potentials and applications. Biocatal Agric Biotechnol. 2020, 25 101599
|
| [22] |
Wang C, Sun J, Lassabliere B, Yu B, Liu SQ. Coffee flavour modification through controlled fermentations of green coffee beans by saccharomyces cerevisiae and Pichia Kluyveri: part I. Effects from individual yeasts. Food Res Int. 2020, 136: 109588
|
| [23] |
Salvetti E, Fondi M, Fani R, Torriani S, Felis GE. Evolution of lactic acid bacteria in the order lactobacillales as depicted by analysis of glycolysis and pentose phosphate pathways. Syst Appl Microbiol. 2013, 36: 291-305
|
| [24] |
Ilmén M, Koivuranta K, Ruohonen L, Rajgarhia V, Suominen P, Penttilä M. Production of L-lactic acid by the yeast candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases. Microb Cell Fact. 2013
|
| [25] |
Song C, Zhang S, Huang H. Choosing a suitable method for the identification of replication origins in microbial genomes. Front Microbiol. 2015, 6: 146676
|
| [26] |
De CarvalhoNeto DP, De ViníciusMelo Pereira G, Finco AMO, Rodrigues C, De Carvalho JC, Soccol JR. Microbiological, physicochemical and sensory studies of coffee beans fermentation conducted in a yeast bioreactor model. Food Biotechnol. 2020, 34: 172-192
|
| [27] |
de Melo Pereira GV, da Silva Vale A, de Carvalho Neto DP, Muynarsk ES, Soccol VT, Soccol CR. Lactic acid bacteria: what coffee industry should know?. Curr Opin Food Sci. 2020, 31: 1-8
|
| [28] |
Bressani APP, Batista NN, Ferreira G, Martinez SJ, Simão JBP, Dias DR, Schwan RF. Characterization of bioactive, chemical, and sensory compounds from fermented coffees with different yeasts species. Food Res Int. 2021, 150 110755
|
| [29] |
Hadj Salem F, Lebrun M, Mestres C, Sieczkowski N, Boulanger R, Collignan A. Transfer kinetics of labeled aroma compounds from liquid media into coffee beans during simulated wet processing conditions. Food Chem. 2020, 322 126779
|
| [30] |
Gänzle MG, Schwab C. Exploitation of the metabolic potential of lactic acid bacteria for improved quality of gluten-free bread. Sci Gluten-Free Foods Beverages. 2009
|
| [31] |
Mitri S, Koubaa M, Maroun RG, Rossignol T, Nicaud JM, Louka N. Bioproduction of 2-phenylethanol through yeast fermentation on synthetic media and on agro-industrial waste and by-products: a review. Foods. 2022, 11: 109
|
Funding
Conselho Nacional de Desenvolvimento Científico e Tecnológico(440343/2022-4)
RIGHTS & PERMISSIONS
Jiangnan University
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