Construction of short synthetic promoters for optimization of ovalbumin expression level in <i>Saccharomyces cerevisiae</i>

doi:10.1007/s43393-024-00250-2

Systems Microbiology and Biomanufacturing ›› 2024, Vol. 4 ›› Issue (3) : 996-1005. DOI: 10.1007/s43393-024-00250-2

Original Article

Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae

Ying Lin¹^,²^,³^,⁴^,⁶, Xiaomin Dong¹^,²^,³^,⁴, Xueqin Lv¹^,²^,³^,⁴, Long Liu¹^,²^,³^,⁴, Jianghua Li¹^,²^,³^,⁴^,⁶, Guocheng Du¹^,²^,³^,⁴^,⁶, Jian Chen²^,³^,⁴^,⁵^,⁶, Yanfeng Liu¹^,²^,³^,⁴^,⁵^,⁶^,^h()

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Abstract

Highlights

Fluorescent protein optimized for promoter fluorescence intensity in S. cerevisiae.

Short promoter library with different strengths and stable expression constructed.

These promoters express ovalbumin fusion fluorescent proteins in S. cerevisiae.

Keywords

Saccharomyces cerevisiae / Promoter engineering / Synthetic promoters / Ovalbumin

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Ying Lin, Xiaomin Dong, Xueqin Lv, Long Liu, Jianghua Li, Guocheng Du, Jian Chen, Yanfeng Liu. Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae. Systems Microbiology and Biomanufacturing, 2024, 4(3): 996‒1005 https://doi.org/10.1007/s43393-024-00250-2

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Funding

National Key Research and Development Program of China(2020YFA0908300); National Natural Science Foundation of China(32222069); Foundation for Innovative Research Groups of the National Natural Science Foundation of China(32021005)