Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae

Ying Lin; Xiaomin Dong; Xueqin Lv; Long Liu; Jianghua Li; Guocheng Du; Jian Chen; Yanfeng Liu

doi:10.1007/s43393-024-00250-2

Systems Microbiology and Biomanufacturing ›› 2024, Vol. 4 ›› Issue (3) : 996 -1005. DOI: 10.1007/s43393-024-00250-2

Original Article

Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae

Ying Lin ¹^,²^,³^,⁴^,⁶
, Xiaomin Dong ¹^,²^,³^,⁴
, Xueqin Lv ¹^,²^,³^,⁴
, Long Liu ¹^,²^,³^,⁴
, Jianghua Li ¹^,²^,³^,⁴^,⁶
, Guocheng Du ¹^,²^,³^,⁴^,⁶
, Jian Chen ²^,³^,⁴^,⁵^,⁶
, Yanfeng Liu ¹^,²^,³^,⁴^,⁵^,⁶^,^h

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Abstract

Fluorescent protein optimized for promoter fluorescence intensity in S. cerevisiae.

Short promoter library with different strengths and stable expression constructed.

These promoters express ovalbumin fusion fluorescent proteins in S. cerevisiae.

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Ying Lin, Xiaomin Dong, Xueqin Lv, Long Liu, Jianghua Li, Guocheng Du, Jian Chen, Yanfeng Liu. Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae. Systems Microbiology and Biomanufacturing, 2024, 4(3): 996-1005 DOI:10.1007/s43393-024-00250-2