Enhanced maltotetraose purity in industrial production by a sustainable bio-physical process

Liyuan Jiang; Haocun Kong; Xiaofeng Ban; Zhengbiao Gu; Caiming Li; Yue-E Sun; Zhaofeng Li

doi:10.1007/s43393-024-00243-1

Systems Microbiology and Biomanufacturing ›› 2024, Vol. 4 ›› Issue (3) : 1028-1038. DOI: 10.1007/s43393-024-00243-1

Original Article

Enhanced maltotetraose purity in industrial production by a sustainable bio-physical process

Liyuan Jiang² ,
Haocun Kong² ,
Xiaofeng Ban² ,
Zhengbiao Gu¹^,²^,³ ,
Caiming Li¹^,²^,³ ,
Yue-E Sun⁴ ,
Zhaofeng Li¹^,²^,³^,^g

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Abstract

Maltotetraose (G4) consists of four glucose units linked by an α-1,4-glycosidic bond. This compound demonstrates remarkable versatility in food processing and exhibits specific physiological functions, suggesting promising applications in the medical, chemical, and food sectors. However, due to the closely related physical and chemical properties of maltotriose (G3), G4, and maltopentose (G5), achieving high-purity G4 has been challenging, resulting in a staggering price of US $438.88 p e r g r a m . I n t h i s s t u d y, a n o v e l a n d e f f i c i e n t b i o - p h y s i c a l m e t h o d w a s d e v e l o p e d t o p r o d u c e h i g h - p u r i t y G 4. I n i t i a l l y, m u l t i - e n z y m a t i c h y d r o l y s i s y i e l d e d G 4 a t a 65.83$ 0.013 per gram, representing that the actual selling price of G4 was 33,760 times the production cost under this process.

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Liyuan Jiang, Haocun Kong, Xiaofeng Ban, Zhengbiao Gu, Caiming Li, Yue-E Sun, Zhaofeng Li. Enhanced maltotetraose purity in industrial production by a sustainable bio-physical process. Systems Microbiology and Biomanufacturing, 2024, 4(3): 1028‒1038 https://doi.org/10.1007/s43393-024-00243-1

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Funding

National Natural Science Foundation of China,(No. 32272263); Science and Technology Support Program of Jiangsu Province,(BE2022323); Collaborative Innovationcenter of Food Safety and Quality Control in Jiangsu Province,(2022-2-3)