Production of mevalonate in Pseudomonas putida via tuning the expression of pathway gene

Abstract

Beyond its potential for biofuel production, Pseudomonas putida’s capability to metabolize lignin and other lignocellulosic materials earmarks it as a pivotal candidate for engineering to yield diverse value-added chemicals, thereby challenging traditional petrochemical approaches. Recognizing the inherent environmental, economic, and societal advantages, amplifying role of P. putida in industrial applications becomes imperative. In this context, our study focused on characterizing a comprehensive set of promoters and ribosome binding site tailored for P. putida, spanning a broad spectrum of activities. By leveraging these genetic tools, we adeptly balanced the heterologous mevalonate (MVA) pathway flux within P. putida. As a culmination of our efforts, the optimal MVA-producing strains were identified, achieving a remarkable yield of 5 g/LMVA in a 5 L fed-batch fermenter, marking the highest reported yield in Pseudomonas to date. This research not only provides valuable genetic tools for future engineering studies with P. putida, but also accentuates P. putida’s potential in synthetic biology and its promise for sustainable chemical production.