In vivo assessment of antimicrobial, antioxidant, antigenotoxic and antimutagenic effects of different extracts from Camellia sinensis

Abstract

In the current study, the phytochemical composition and biological activity (antimicrobial, antioxidant, antigenotoxic, and antimutagenic) of different leaf extracts of green tea (Camellia Sinensis) were investigated. Maximum total polyphenol content (17.12 ± 0.02), total flavonoid content (16.18 ± 0.03), antioxidant activity (DPPH) to scavenge free radicals (95.9%) and highest antimicrobial activity against Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration value of 3.12 mg/ml and a 26 mm zone of inhibition were recorded for the methanolic extract. The antigenotoxic potential of various extracts of C. sinensis at a concentration of 1000 µg/ml reduced DNA damaged by 37% against Cd-induced genotoxicity as indicated by tail moment (7.9 ± 0.4) during comet assay. Antimutagenic potential of methanolic extracts showed a mutagenic inhibition of 52% against sodium azide mutagen in TA98 strain (+ S9) during the ames test. Gas chromatography–mass spectrometry analysis of methanolic extract showed the presence of a number of compounds The above findings suggest the potential application of C. sinensis methanol extract in food and the health business, and to meet expanding human requirements.