In vivo assessment of antimicrobial, antioxidant, antigenotoxic and antimutagenic effects of different extracts from Camellia sinensis

Abstract

In the current study, the phytochemical composition and biological activity (antimicrobial, antioxidant, antigenotoxic, and antimutagenic) of different leaf extracts of green tea (Camellia Sinensis) were investigated. Maximum total polyphenol content (17.12 ± 0.02), total flavonoid content (16.18 ± 0.03), antioxidant activity (DPPH) to scavenge free radicals (95.9%) and highest antimicrobial activity against Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration value of 3.12 mg/ml and a 26 mm zone of inhibition were recorded for the methanolic extract. The antigenotoxic potential of various extracts of C. sinensis at a concentration of 1000 µg/ml reduced DNA damaged by 37% against Cd-induced genotoxicity as indicated by tail moment (7.9 ± 0.4) during comet assay. Antimutagenic potential of methanolic extracts showed a mutagenic inhibition of 52% against sodium azide mutagen in TA98 strain (+ S9) during the ames test. Gas chromatography–mass spectrometry analysis of methanolic extract showed the presence of a number of compounds The above findings suggest the potential application of C. sinensis methanol extract in food and the health business, and to meet expanding human requirements.

Keywords

Green tea / Antimutagenic / Antigenotoxicity / Camellia sinensis / Antimicrobial / Antioxidant

References

[1] Lambert JD, Sang S, Yang CS. Biotransformation of green tea polyphenols and the biological activities of those metabolites. Mol Pharm, 2007, 4(6): 819-825,

[2] Meegahakumbura MK, Wambulwa MC, Li MM, Thapa KK, Sun YS, Möller M, Xu JC, Yang JB, Liu J, Liu BY, Li DZ. Domestication origin and breeding history of the tea plant (Camellia sinensis) in China and India based on nuclear microsatellites and cpDNA sequence data. Front Plant Sci, 2018, 8: 2270,

[3] Zhao T, Li C, Wang S, Song X. Green tea (Camellia sinensis): a review of its phytochemistry, pharmacology, and toxicology. Molecules, 2022, 27(12): 3909,

[4] Schönthal AH. Adverse effects of concentrated green tea extracts. Mol Nutr Food Res, 2011, 55(6): 874-885,

[5] Suzuki Y, Miyoshi N, Isemura M. Health-promoting effects of green tea. Proc Jpn Acad Series B, 2012, 88(3): 88-101,

[6] Khan N, Mukhtar H. Cancer and metastasis: prevention and treatment by green tea. Cancer Metastasis Rev, 2010, 29: 435-445,

[7] Lange KW. Tea in cardiovascular health and disease: a critical appraisal of the evidence. Food Sci Human Wellness, 2022, 11(3): 445-454,

[8] Shareef SH, Ibrahim IA, Alzahrani AR, Al-Medhtiy MH, Abdulla MA. Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-induced liver injury in Sprague Dawley rats. Saudi J Biol Sci, 2022, 29(1): 564-573,

[9] Esmaeelpanah E, Rahmatkhah A, Poormahmood N, Razavi BM, Hasani FV, Hosseinzadeh H. Protective effect of green tea aqueous extract on acrylamide induced neurotoxicity. Jundishapur J Nat Pharm Prod, 2015,

[10] Esmaeelpanah E, Razavi BM, VahdatiHasani F, Hosseinzadeh H. Evaluation of epigallocatechin gallate and epicatechin gallate effects on acrylamide-induced neurotoxicity in rats and cytotoxicity in PC 12 cells. Drug Chem Toxicol, 2018, 41(4): 441-448,

[11] Grzesik M, Naparło K, Bartosz G, Sadowska-Bartosz I. Antioxidant properties of catechins: comparison with other antioxidants. Food Chem, 2018, 241: 480-492,

[12] Youn HS, Lee JY, Saitoh SI, Miyake K, Kang KW, Choi YJ, Hwang DH. Suppression of MyD88-and TRIF-dependent signaling pathways of Toll-like receptor by (−)-epigallocatechin-3-gallate, a polyphenol component of green tea. Biochem Pharmacol, 2006, 72(7): 850-859,

[13] Bernatoniene J, Kopustinskiene DM. The role of catechins in cellular responses to oxidative stress. Molecules, 2018, 23(4): 965,

[14] Archana S, Abraham J. Comparative analysis of antimicrobial activity of leaf extracts from fresh green tea, commercial green tea and black tea on pathogens. J Appl Pharm Sci. 2011 Oct 30(Issue):149–52.

[15] Tariq AL, Reyaz AL. Phytochemical analysis of Camellia sinensis leaves. Int J Drug Dev Res, 2012, 4(4): 311-316

[16] Khan FA, Hussain I, Farooq S, Ahmad M, Arif M, Rehman IU. Phytochemical screening of some Pakistanian medicinal plants. Middle-East J Sci Res, 2011, 8(3): 575-578

[17] Subhashini R, Rao UM, Sumathi P, Gunalan G. A comparative phytochemical analysis of cocoa and green tea. Indian J Sci Technol, 2010, 3(2): 188-192,

[18] Manandhar S, Luitel S, Dahal RK. In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. J Trop Medicine, 2019, 2019: 1,

[19] Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J Ethnopharmacol, 2001, 78(2–3): 119-127,

[20] Azzahra LF, Fouzia H, Mohammed L, Noureddine B. Antioxidant response of Camellia sinensis and Rosmarinus officinalis aqueous extracts toward H2O2 stressed mice. J Appl Pharm Sci, 2012, 2(7): 70-76

[21] Yadav B, Vishwakarma V, Kumar S, Aggarwal NK, Gupta R, Yadav A. Antigenotoxic effects of morin against lead induced genomic damage in cultured human peripheral blood lymphocytes. J Food Biochem, 2019, 43(7),

[22] Gautam V, Sharma A, Arora S, Bhardwaj R, Ahmad A, Ahamad B, Ahmad P. In-vitro antioxidant, antimutagenic and cancer cell growth inhibition activities of Rhododendron arboreum leaves and flowers. Saudi J Biol Sci, 2020, 27(7): 1788-1796,

[23] Dhawan D, Gupta J. Research article comparison of different solvents for phytochemical extraction potential from datura metel plant leaves. Int J Biol Chem, 2017, 11(1): 17-22,

[24] Giri RS, Dhanalakshmi S. Isolation and characterization of endosymbiont of Pithecellobium dulce (Benth). J Med Plants, 2015, 3(3): 26-27

[25] Tiwari RP, Bharti SK, Kaur HD, Dikshit RP, Hoondal GS. Synergistic antimicrobial activity of tea and antibiotics. Indian J Med Res, 2005, 122(1): 80

[26] Pradhan S, Dubey RC. Evaluation of phytochemical, antimicrobial and time-killing assay of Camellia species. Vegetos, 2020, 33(4): 759-765,

[27] Nibir YM, Sumit AF, Akhand AA, Ahsan N, Hossain MS. Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pac J Trop Biomed, 2017, 7(4): 352-357,

[28] Yam TS, Shah S, Hamilton-Miller JM. Microbiological activity of whole and fractionated crude extracts of tea (Camellia sinensis), and of tea components. FEMS Microbiol Lett, 1997, 152(1): 169-174,

[29] Bandyopadhyay D, Chatterjee TK, Dasgupta A, Lourduraja J, Dastidar SG. In vitro and in vivo antimicrobial action of tea: the commonest beverage of Asia. Biol Pharm Bull, 2005, 28(11): 2125-2127,

[30] Shah T, Shaikh F, Sarah B, Sheikh K, Ansari S. Role of Green Tea on Lipid Profile of Normal and Hyperlipidemic Individuals. J Liaquat Univ Med Health Sci, 2018, 17(04): 225-229,

[31] Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. Antioxidant activity of some common plants. Turk J Biol, 2008, 32(1): 51-55

[32] Rahardja F. Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fraction. Biomed Eng, 2015, 1(1): 1-6

[33] Hossain MM, Mahmood S. In vitro studies on antibacterial, thrombolytic and antioxidant activities of green tea or Camellia sinensis. Am J Phytomed Clinic Therap, 2014, 2: 1200-1211

[34] Han KC, Wong WC, Benzie IF. Genoprotective effects of green tea (Camellia sinensis) in human subjects: results of a controlled supplementation trial. Br J Nutr, 2011, 105(2): 171-179,

[35] Vasiljevic J, Zivkovic L, Cabarkapa A, Bajic V, Djelic N, Spremo-Potparevic B. Cordyceps sinensis: genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay. Alt Ther Health Med, 2016, 22: 24

[36] Makhafola TJ, Elgorashi EE, McGaw LJ, Verschaeve L, Eloff JN. The correlation between antimutagenic activity and total phenolic content of extracts of 31 plant species with high antioxidant activity. BMC Complement Altern Med, 2016, 16(1): 1-3,

[37] Zahin M, Bokhari NA, Ahmad I, Husain FM, Althubiani AS, Alruways MW, Perveen K, Shalawi M. Antioxidant, antibacterial, and antimutagenic activity of Piper nigrum seeds extracts. Saudi J Biol Sci, 2021, 28(9): 5094-5105,

[38] Henning SM, Fajardo-Lira C, Lee HW, Youssefian AA, Go VL, Heber D. Catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity. Nutr Cancer, 2003, 45(2): 226-235,

[39] Gupta S, Saha B, Giri AK. Comparative antimutagenic and anticlastogenic effects of green tea and black tea: a review. Mutat Res/Rev Mutat Res, 2002, 512(1): 37-65,

[40] Cebi N, Erarslan A. Determination of the antifungal, antibacterial activity and volatile compound composition of citrus bergamia peel essential oil. Foods, 2023, 12(1): 203,

[41] Fathi R, Abd Allah D, Helmy I. Effect of Green tea and black seed on methotrexate induced cytotoxicity of the oral mucosa, tongue and the submandibular salivary gland of Albino rats. Egypt Dent J, 2023, 69(1): 413-426,

[42] Hope O, Bright IE, Alagbonsi AI. GC-MS biocomponents characterization and antibacterial potency of ethanolic crude extracts of Camellia sinensis. SAGE Open Med, 2022, 10: 20503121221116859,

[43] Olivia NU, Goodness UC, Obinna OM. Phytochemical profiling and GC-MS analysis of aqueous methanol fraction of Hibiscus asper leaves. Future J Pharm Sci, 2021, 7: 1-5