Screening and evaluation of Cr(VI) biosorption potential of metal-resistant bacteria isolated from Sukinda chromite mine soil of Odisha

Abstract

Chromite mine soil is highly contaminated with different heavy metals like chromium, cadmium, lead, and arsenic. In the present study, 42 numbers of metal-tolerant bacteria (Cr-14, Cd-9, Pb-11, and As-8) were isolated from three soil samples collected from chromite mine soils of Sukinda, Odisha using nutrient broth medium supplemented with 50 mg/L of each of the above four metals. The isolated metal-tolerant bacteria were screened using increased above mentioned metal ion concentrations (50, 100 ppm, 200, 500, 700, and 1000 mg/L) to know the maximum resistance of the bacteria towards a particular metal. Out of the 42 predominant bacterial isolates, 4 bacteria (CrS₂C₇, PbS₁M₁, CdS₂M₁ and AsS₁M₂) showing high resistance to respective metal ions were subjected to biochemical characterization followed by 16S rRNA sequencing and identified as Pseudomonas argentinensis (CrS₂C₇), Bacillus subtilis (PbS₁M₁), Bacillus thuringiensis (CdS₂M₁), and Bacillus fungorum (AsS₁M₂). Further, the Cr⁺⁶ biosorption potential of these 4 bacteria (CrS₂C₇, PbS₁M₁, AsS₁M₂, and CdS₂M₁) were evaluated using 100 mg/L K₂Cr₂O₇ metal solution. Out of the above four isolates, P. argentinensis showed highest biosorption of 73.4% for Cr⁺⁶ metal ion solution The present finding of Cr⁺⁶ biosorption by P. argentinensis indicates that it can be used as an effective biosorbent for biosorption of Cr⁺⁶ from chromate contaminated aqueous environment.

Keywords

Chromite mine soil / Heavy metal pollution / Chromate biosorption / Metal-tolerant bacteria / Phylogenetic analyses

References

1.	Medfu Tarekegn M, Zewdu Salilih F, Ishetu AI. Microbes used as a tool for bioremediation of heavy metal from the environment. Cogent Food & Agriculture, 2020, 6(1): 1783174,
2.	Mishra, S., Bharagava, R.N., More, N., Yadav, A., Zainith, S., Mani, S. and Chowdhary, P., 2019. Heavy metal contamination: an alarming threat to environment and human health.?Environmental biotechnology: For sustainable future, pp.103–125
3.	Shrestha R, Ban S, Devkota S, Sharma S, Joshi R, Tiwari AP, Kim HY, Joshi MK. Technological trends in heavy metals removal from industrial wastewater: a review. J Environ Chem Eng, 2021, 9(4): 105688,
4.	Marsh TL, McInerney MJ. Relationship of hydrogen bioavailability to chromate reduction in aquifer sediments. Appl Environ Microbiol, 2001, 67(4): 1517-1521, pmcid: 92763
5.	DesMarias TL, Costa M. Mechanisms of chromium-induced toxicity. Current Opinion Toxicol, 2019, 14: 1-7,
6.	Sukhn C, Awwad J, Ghantous A, Zaatari G. Associations of semen quality with non-essential heavy metals in blood and seminal fluid: data from the Environment and Male Infertility (EMI) study in Lebanon. J Assist Reprod Genet, 2018, 35: 1691-1701, pmcid: 6133818
7.	Sanders T, Liu Y, Buchner V, Tchounwou PB. Neurotoxic effects and biomarkers of lead exposure: a review. Rev Environ Health, 2009, 24(1): 15-46, pmcid: 2858639
8.	Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M.R. and Sadeghi, M., 2021. Toxic mechanisms of five heavy metals: mercury, lead, chromium, cadmium, and arsenic. Frontiers in pharmacology, pp.227
9.	Bertin G, Averbeck D. Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review). Biochimie, 2006, 88(11): 1549-1559,
10.	Compounds, C., 1993. In Beryllium, Cadmium, Mercury and Exposure in the Glass Manufacturing Industry.?IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Series.
11.	Shaji E, Santosh M, Sarath KV, Prakash P, Deepchand V, Divya BV. Arsenic contamination of groundwater: A global synopsis with focus on the Indian Peninsula. Geosci Front, 2021, 12(3),
12.	WHO (2018) Factsheet on drinking-water.?http://www.who.int/news-room/fact-sheets/detail/drinking-water
13.	IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, World Health Organization and International Agency for Research on Cancer, 2004.?Some drinking-water disinfectants and contaminants, including arsenic?(Vol. 84). IARC.
14.	Fatoki, J.O. and Badmus, J.A., 2022. Arsenic as an environmental and human health antagonist: A review of its toxicity and disease initiation. Journal of Hazardous Materials Advances, p.100052.
15.	Chappell WR, Beck BD, Brown KG, Chaney R, Cothern R, Cothern CR, Irgolic KJ, North DW, Thornton I, Tsongas TA. Inorganic arsenic: a need and an opportunity to improve risk assessment. Environ Health Perspect, 1997, 105(10): 1060-1067, pmcid: 1470381
16.	Hussain N, Goswami L, Bhattacharya SS. Microbial Detoxification of Contaminated Land. Bio-Inspired Land Remediat, 2023, 2: 189-219,
17.	Gavrilescu M. Removal of heavy metals from the environment by biosorption. Eng Life Sci, 2004, 4(3): 219-232,
18.	Sherameti I, Varma A. . Detoxification of heavy metals, 2011 Springer Science & Business Media,
19.	Choudhury R, Srivastava S. Zinc resistance mechanisms in bacteria. Current Sci, 2001, 10: 768-775
20.	Bruins MR, Kapil S, Oehme FW. Microbial resistance to metals in the environment. Ecotoxicol Environ Saf, 2000, 45(3): 198-207,
21.	Cohen RR. Use of microbes for cost reduction of metal removal from metals and mining industry waste streams. J Clean Prod, 2006, 14(12–13): 1146-1157,
22.	Dhakate R, Singh VS, Hodlur GK. Impact assessment of chromite mining on groundwater through simulation modeling study in Sukinda chromite mining area, Orissa. India J Hazard Mater, 2008, 160(2–3): 535-547,
23.	Rao, A.V., Dhakate, R.R., Singh, V.S. and Jain, S.C., 2003.?Geophysical and hydrogeological investigations to delineate aquifer geometry at kaliapani, Sukinda, Orissa. NGRI technical report no. GW-367
24.	Zobell CE. Action of micro?rganisms on hydrocarbons. Bacteriol Rev, 1946, 10(1–2): 1-49, pmcid: 440899
25.	Jackson ML. Soil chemical analysis, pentice hall of India Pvt. Ltd. New Delhi India, 1973, 498: 151-154
26.	Masood F, Malik A. Hexavalent chromium reduction by Bacillus sp. strain FM1 isolated from heavy-metal contaminated soil. Bull Environ Contam Toxicol, 2011, 86: 114-119,
27.	Jain PK, Ramachandran S, Shukla V, Bhakuni D, Verma SK. Characterization of metal and antibiotic resistance in a bacterial population isolated from copper mining industry. Int J Integrative Biol, 2009, 6: 57-61
28.	Holt, J.G., Krieg, N.R., Sneath, P.H., Staley, J.T. and Williams, S.T., 1994. Bergey’s Manual of determinate bacteriology.
29.	Collins MD, Falsen E, Brownlee K, Lawson PA. Helcococcus sueciensis sp. Nov., isolated from a human wound. Int J Sys Evolutionary Microbiol, 2004, 54(5): 1557-1560,
30.	Kapley A, Purohit HJ. Tracking of phenol degrading genotype. Environ Sci Pollut Res, 2001, 8: 89-90,
31.	Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011, 28(10): 2731-2739, pmcid: 3203626
32.	Olukanni DO, Agunwamba JC, Ugwu EI. Biosorption of heavy metals in industrial wastewater using micro-organisms (Pseudomonas aeruginosa). Am J Scient Ind Res, 2014, 5(2): 81-87
33.	Jan FA, Ishaq M, Khan S, Ihsanullah I, Ahmad I, Shakirullah M. A comparative study of human health risks via consumption of food crops grown on wastewater irrigated soil (Peshawar) and relatively clean water irrigated soil (lower Dir). J Hazard Mater, 2010, 179(1–3): 612-621,
34.	Wu SC, Luo YM, Cheung KC, Wong MH. Influence of bacteria on Pb and Zn speciation, mobility and bioavailability in soil: a laboratory study. Environ Pollut, 2006, 144(3): 765-773,
35.	Peix A, Berge O, Rivas R, Abril A, Velázquez E. Pseudomonas argentinensis sp. Nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Cordoba, Argentina. Int J Syst Evolut Microbiol, 2005, 55(3): 1107-1112,
36.	Zhang C, Li J, Chen L, Shi X, Chen B, Lv X, Ni L. Effects of alkali, enzymes, and ultrasound on monosodium glutamate byproduct for a sustainable production of Bacillus subtilis. Food Chem, 2021, 360: 129967,