The function of steroid terminal monooxygenase in cholesterol or phytosterol degradation by the Mycobacterium neoaurum NRRL B-3805

Abstract

Cholesterol and phytosterols can be served as the substrates for Actinobacteria to produce the precursors used in the synthesis of steroidal medicines. Cytochrome P450s from CYP125 and CYP142 families initiates the metabolism of cholesterol and/or phytosterols. In this study, we demonstrate the functional redundancy and substrate preference of five cytochrome CYP125s and CYP142s by the unmarked cyp genes deletion strains of Mycobacterium neoaurum NRRL B-3805. CYP125-3 was found to be responsible for the catalytic hydroxylation of both cholesterol and the C24-branched side-chains of phytosterols, while CYP142-2 can only oxidize cholesterol and/or cholest-4-en-3-one. The strain harboring the CYP125-3 produced 6.47 g l^− 1 and 5.13 g l^− 1 of 4-androstene-3,17-dione (AD) when 15 g l^− 1 cholesterol or 17.5 g l^− 1 phytosterols used, respectively. While, the strain harboring the CYP142-2 produced 7.10 g l^− 1 AD when 15 g l^− 1 cholesterol used. The other three cytochrome P450s have a smaller contribution for the degradation of cholesterol and phytosterols. CYP125-3 has the hydroxylation activity on more substrates than that of CYP142-2. This study provided possible guidance to improve the efficiency of AD and other metabolites production from different kinds of sterols by the genetic manipulation.

Funding

National Key R & D Program of China,(2019YFA0905300)

National Natural Science Foundation of China,(32171477)

Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project,(TSBICIP-KJGG-001)