Evaluation of the function of a luciferase-like monooxygenase homologue in 4,4´-dithiodibutyric acid catabolism in Rhodococcus erythropolis MI2

Abstract

The bacterium Rhodococcus erythropolis MI2 uses 4,4´-dithiodibutyric acid (DTDB) as carbon source to synthesize polythioesters (PTE). The first step for the production of PTE using DTDB is catalyzed by an NADH:flavin oxidoreductase (nox) as it was previously shown in our laboratory, and the second step is catabolized by a putative luciferase-like monooxygenase (Llm). In the current study, experiments were carried out to identify the function of Llm. Hence, the llm gene, which encodes for the Llm protein, was amplified from the genomic DNA of MI2 using polymerase chain reaction and expressed in Escherichia coli BL21 cells. Protein purification was done using His Spin Trap affinity columns. Enzyme assay was carried out using the purified protein and p-coumaric acid as substrate giving a specific activity of 1.6 U/mg protein for the purified Llm. The responsible gene (llm) was deleted in the genome of MI2, and a single deletion mutant was subsequently generated. Finally, growth of the wild-type strain (MI2) and the mutant strain (MI2Δllm) were compared using DTDB or succinate as carbon sources. Whereas the wild type was successfully grown with DTDB or succinate, the llm-negative mutant exhibited low grow with DTDB although it grows very well with succinate.

Funding

Alexander von Humboldt-Stiftung,(IND 1162665 HFST-P)

Westfälische Wilhelms-Universität Münster (1056),