Coproduction of xylose and biobutanol from corn stover via recycling of sulfuric acid pretreatment solution
Jin-Jun Dong, Bao-Jian Ma, Yong-Mei Liu, Hao Li, Lei Gong, Rui-Zhi Han, Guo-Chao Xu, Ye Ni
Systems Microbiology and Biomanufacturing ›› 2020, Vol. 1 ›› Issue (2) : 200-207.
Coproduction of xylose and biobutanol from corn stover via recycling of sulfuric acid pretreatment solution
Sulfuric acid was used in the pretreatment of corn stover to obtain xylose as a value-added by-product, and the pretreated corn stover (Pre-CS) was hydrolyzed to produce glucose for butanol fermentation. The aim of this work is to achieve high xylose accumulation and reduced wastewater by recycling the pretreatment solution. The pretreatment conditions were optimized as follows: 180 °C, 15 min, 1:7 solid–liquid ratio (w/w), 0.6% H2SO4 (w/w, first batch)/0.9% H2SO4 (w/w, second and third batches), in which pretreatment solution was recycled for three times. Under above conditions, pretreatment solution containing 56.3 g/L xylose and 4.5 g/L glucose was obtained. Pre-CS residue was further hydrolyzed by cellulase to achieve 35.7–39.9 g/L glucose. The condensed corn stover hydrolysate was subjected to simultaneous detoxification and sterilization using 1% (w/w) activated carbon and then applied in butanol fermentation. The highest butanol titer of 9.5 g/L was obtained in 72 h. The results provide a practical approach for coproducing xylose and biobutanol from corn stover.
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