Applications of genetic code expansion technology in eukaryotes

  • Qiao-ru Guo 1 ,
  • Yu J. Cao , 1,2
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  • 1. State Key Laboratory of Chemical Oncogenomic, Guangdong Provincial Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, China
  • 2. Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China
joshuacao@pku.edu.cn

Received date: 04 Jul 2023

Accepted date: 26 Sep 2023

Published date: 15 May 2024

Copyright

2023 The Author(s) 2023. Published by Oxford University Press on behalf of Higher Education Press.

Abstract

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to proteins. In eukaryotes, genetic code expansion (GCE) enables the incorporation of UAAs and facilitates posttranscriptional modification (PTM), which is not feasible in prokaryotic systems. GCE is also a powerful tool for cell or animal imaging, the monitoring of protein interactions in target cells, drug development, and switch regulation. Therefore, there is keen interest in utilizing GCE in eukaryotic systems. This review provides an overview of the application of GCE in eukaryotic systems and discusses current challenges that need to be addressed.

Cite this article

Qiao-ru Guo , Yu J. Cao . Applications of genetic code expansion technology in eukaryotes[J]. Protein & Cell, 2024 , 15(5) : 331 -363 . DOI: 10.1093/procel/pwad051

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