LETTER

Mouse-adapted SARS-CoV-2 replicates efficiently in the upper and lower respiratory tract of BALB/c and C57BL/6J mice

  • Jinliang Wang 1 ,
  • Lei Shuai 1 ,
  • Chong Wang 1 ,
  • Renqiang Liu 1 ,
  • Xijun He 2 ,
  • Xianfeng Zhang 2 ,
  • Ziruo Sun 1 ,
  • Dan Shan 1 ,
  • Jinying Ge 1 ,
  • Xijun Wang 1 ,
  • Ronghong Hua 1 ,
  • Gongxun Zhong , 1 ,
  • Zhiyuan Wen , 1 ,
  • Zhigao Bu , 1,2
Expand
  • 1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
  • 2. National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, China

Published date: 15 Oct 2020

Copyright

2020 The Author(s)

Cite this article

Jinliang Wang , Lei Shuai , Chong Wang , Renqiang Liu , Xijun He , Xianfeng Zhang , Ziruo Sun , Dan Shan , Jinying Ge , Xijun Wang , Ronghong Hua , Gongxun Zhong , Zhiyuan Wen , Zhigao Bu . Mouse-adapted SARS-CoV-2 replicates efficiently in the upper and lower respiratory tract of BALB/c and C57BL/6J mice[J]. Protein & Cell, 2020 , 11(10) : 776 -782 . DOI: 10.1007/s13238-020-00767-x

1
Bao L, Deng W, Huang B, Gao H, Liu J, Ren L, Wei Q, Yu P, Xu Y, Qi F (2020) The pathogenicity of SARS-CoV-1 2 in hACE2 transgenic mice. Nature. https://doi.org/10.1038/s41586-020-2312-y

DOI

2
Beigel JH, Tomashek KM, Dodd LE, Mehta AK, Zingman BS, Kalil AC, Hohmann E, Chu HY, Luetkemeyer A, Kline S (2020) Remdesivir for the treatment of Covid-19- preliminary report. N Engl J Med. https://doi.org/10.1056/NEJMoa2007764

DOI

3
Dinnon KH III, Leist SR, Schafer A, Edwards CE, Martinez DR, Montgomery SA, West A, Yount BL, Hou YJ, Adams LE, Gully KL (2020) A mouse-adapted SARS-CoV-2 model for the evaluation of COVID-19 medical countermeasures. bioRxiv. https://doi.org/10.1101/20200506081497

DOI

4
Gu H, Chen Q, Yang G, He L, Fan H, Deng Y-Q, Wang Y, Teng Y, Zhao Z, Cui Y (2020) Rapid adaptation of SARS-CoV-2 in BALB/c mice: novel mouse model for vaccine efficacy. bioRxiv. https://dottps://doii.org/10.1.org/10.11101/202005001/202005022073410734111

DOI

5
Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, Liu L, Shan H, Lei CL, Hui DSC (2020) Clinical Characteristics of Coronavirus disease 2019 in China. N Engl J Med 382:1708–1720

DOI

6
Hassan AO, Case JB, Winkler ES, Thackray L, Kafai NM, Bailey AL, McCune BT, Fox JM, Chen RE, Soussi WBA (2020) A SARS-CoV-2 infection model in mice demonstrates protection by neutralizing antibodies. Cell. https://doi.org/10.1016/jcell202006011

DOI

7
Jiang R-D, Liu M-Q, Chen Y, Shan C, Zhou Y-W, Shen X-R, Li Q, Zhang L, Zhu Y, Si H-R (2020) Pathogenesis of SARS-CoV-2 in transgenic mice expressing human angiotensinconverting enzyme 2. Cell. https://doi.org/10.1016/jcell202005027

DOI

8
Kirchdoerfer RN, Ward AB (2019) Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors. Nat Commun 10:2342

DOI

9
Liu Z, Zheng H, Yuan R, Li M, Lin H, Peng J, Xiong Q, Sun J, Li B, Wu J (2020) Identification of a common deletion in the spike protein of SARS-CoV-2. bioRxiv. https://doi.org/10.1101/20200331015941

DOI

10
Sun J, Zhuang Z, Zheng J, Li K, Wong RL, Liu D, Huang J, He J, Zhu A, Zhao J, Li X (2020) Generation of a broadly useful model for COVID-19 pathogenesis vaccination, and treatment. Cell. https://doi.org/10.1016/jcell202006010

DOI

11
Wang M, Cao R, Zhang L, Yang X, Liu J, Xu M, Shi Z, Hu Z, Zhong W, Xiao G (2020a) Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Res 30:269–271

DOI

12
Wang Y, Zhang D, Du G, Du R, Zhao J, Jin Y, Fu S, Gao L, Cheng Z, Lu Q (2020b) Remdesivir in adults with severe COVID-19: a randomised, double-blind, placebo-controlled, multicentre trial. Lancet 395:1569–1578

13
World Health Organization (2020) Coronavirus disease (COVID-2019) situation report for 30 June 2020. https://www.who.int/docs/default-source/coronaviruse/20200630-covid-19-sitrep-162.pdf?sfvrsn=e00a5466_2

14
Zhou H, Chen X, Hu T, Li J, Song H, Liu Y, Wang P, Liu D, Yang J, Holmes EC (2020) A novel bat coronavirus reveals natural insertions at the S1/S2 cleavage site of the Spike protein and a possible recombinant origin of HCoV-19. bioRxiv. https://doi.org/10.1101/20200302974139

DOI

Outlines

/