RESEARCH ARTICLE

Identification of natural compounds targeting Annexin A2 with an anti-cancer effect

  • Yu-Shi Wang ,
  • He Li ,
  • Yang Li ,
  • Hongyan Zhu ,
  • Ying-Hua Jin
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  • Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China

Received date: 07 Nov 2017

Accepted date: 24 Jan 2018

Published date: 11 Jun 2018

Copyright

2018 The Author(s) 2018. This article is an open access publication

Abstract

Annexin A2, a multifunctional tumor associated protein, promotes nuclear factor-kappa B (NF-κB) activation by interacting with NF-κB p50 subunit and facilitating its nuclear translocation. Here we demonstrated that two ginsenosides Rg5 (G-Rg5) and Rk1 (G-Rk1), with similar structure, directly bound to Annexin A2 by molecular docking and cellular thermal shift assay. Both Rg5 and Rk1 inhibited the interaction between Annexin A2 and NF-κB p50 subunit, their translocation to nuclear and NF-κB activation. Inhibition of NF-κB by these two ginsenosides decreased the expression of inhibitor of apoptosis proteins (IAPs), leading to caspase activation and apoptosis. Over expression of K302A Annexin A2, a mutant version of Annexin A2, which fails to interact with G-Rg5 and G-Rk1, effectively reduced the NF-κB inhibitory effect and apoptosis induced by G-Rg5 and G-Rk1. In addition, the knockdown of Annexin A2 largely enhanced NF-κB activation and apoptosis induced by the two molecules, indicating that the effects of G-Rg5 and G-Rk1 on NF-κB were mainly mediated by Annexin A2. Taken together, this study for the first time demonstrated that G-Rg5 and G-Rk1 inhibit tumor cell growth by targeting Annexin A2 and NF-κB pathway, and G-Rg5 and G-Rk1 might be promising natural compounds for targeted cancer therapy.

Key words: Annexin A2; G-Rg5; G-Rk1; HCC; NF-κB

Cite this article

Yu-Shi Wang , He Li , Yang Li , Hongyan Zhu , Ying-Hua Jin . Identification of natural compounds targeting Annexin A2 with an anti-cancer effect[J]. Protein & Cell, 2018 , 9(6) : 568 -579 . DOI: 10.1007/s13238-018-0513-z

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