LETTER

AlphaLISA detection of alpha-synuclein in the cerebrospinal fluid and its potential application in Parkinson’s disease diagnosis

  • Hongli Zhao 1 ,
  • Jue Zhao 1 ,
  • Jiapeng Hou 1 ,
  • Siqing Wang 2 ,
  • Yu Ding , 1 ,
  • Boxun Lu , 1,3 ,
  • Jian Wang , 1
Expand
  • 1. State Key Laboratory of Medical Neurobiology, Department of Neurology in Huashan Hospital, School of Life Sciences, National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai 200040, China
  • 2. Changzhou Furuite Biological Technology Co. Ltd., Changzhou 213145, China
  • 3. Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China

Published date: 20 Sep 2017

Copyright

2017 The Author(s) 2017. This article is an open access publication

Cite this article

Hongli Zhao , Jue Zhao , Jiapeng Hou , Siqing Wang , Yu Ding , Boxun Lu , Jian Wang . AlphaLISA detection of alpha-synuclein in the cerebrospinal fluid and its potential application in Parkinson’s disease diagnosis[J]. Protein & Cell, 2017 , 8(9) : 696 -700 . DOI: 10.1007/s13238-017-0424-4

1
AaslyJO, JohansenKK, BronstadG, WaroBJ, MajbourNK, VargheseS, AlzahmiF, PaleologouKE, AmerDA, Al-HayaniA, El-AgnafOM (2014) Elevated levels of cerebrospinal fluid alphasynuclein oligomers in healthy asymptomatic LRRK2 mutation carriers.Front Aging Neurosci6:248

DOI

2
BartelsT, ChoiJG, SelkoeDJ (2011) α-Synuclein occurs physiologically as a helically folded tetramer that resists aggregation.Nature477(7362):107–110

DOI

3
BerrocalR, VasquezV, KrsSR, GadadBS, KsR (2014) α-Synuclein Misfolding Versus Aggregation Relevance to Parkinson’s Disease: Critical Assessment and Modeling.Molecular Neurobiology51(3):1417–1431

DOI

4
BidinostiM, ShimshekDR, MollenhauerB, MarcellinD, SchweizerT, LotzGP, SchlossmacherMG, WeissA (2012) Novel one-step immunoassays to quantify α-synuclein: applications for biomarker development and high-throughput screening.Journal of Biological Chemistry287(40):33691–33705

DOI

5
Bielefeld-SevignyM (2009) AlphaLISA immunoassay platform- the “no-wash” high-throughput alternative to ELISA.Assay Drug Dev Technol7(1):90–92

DOI

6
De GenstEJ, GuilliamsT, WellensJ, O’DayEM, WaudbyCA, MeehanS, DumoulinM, HsuS-TD, CremadesN, VerschuerenKHG, PardonE, WynsL, SteyaertJ, ChristodoulouJ, DobsonCM (2010) Structure and Properties of a Complex of α-Synuclein and a Single-Domain Camelid Antibody.Journal of Molecular Biology402(2):326–343

DOI

7
DelenclosM, JonesDR, McLeanPJ, UittiRJ (2016) Biomarkers in Parkinson’s disease: Advances and strategies.Parkinsonism Relat Disord22(Suppl 1):S106–S110

DOI

8
El-AgnafOMA (2003) α-Synuclein implicated in Parkinson’s disease is present in extracellular biological fluids, including human plasma.FASEB J17(13):1945–1947

DOI

9
KimD, PaikJH, ShinD-W, KimH-S, ParkC-S, KangJ-H (2014) What is the Clinical Significance of Cerebrospinal Fluid Biomarkers in Parkinson’s disease? Is the Significance Diagnostic or Prognostic?Experimental Neurobiology23(4):352

DOI

10
LandeckN, HallH, ArdahMT, MajbourNK, El-AgnafOM, HallidayG, KirikD (2016) A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alphasynuclein.Mol Neurodegener11(1):61

DOI

11
MajbourNK, VaikathNN, van DijkKD, ArdahMT, VargheseS, VesteragerLB, MontezinhoLP, PooleS, Safieh-GarabedianB, TokudaT, TeunissenCE, BerendseHW, van de BergWD, El-AgnafOM (2016) Oligomeric and phosphorylated alpha-synuclein as potential CSF biomarkers for Parkinson’s disease.Mol Neurodegener11:7

DOI

Outlines

/