miR-27b inhibits gastric cancer metastasis by targeting NR2F2
Received date: 26 Jun 2016
Accepted date: 20 Oct 2016
Published date: 17 Mar 2017
Copyright
Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitroand in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells’ migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells’ proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients’ survival.
Key words： miR-27b; NR2F2; gastric cancer; tumor metastasis
Qingzhao Feng , Xionglin Wu , Fuchao Li , Beibei Ning , Xiaofeng Lu , Yin Zhang , Ying Pan , Wenxian Guan . miR-27b inhibits gastric cancer metastasis by targeting NR2F2[J]. Protein & Cell, 2017 , 8(2) : 114 -122 . DOI: 10.1007/s13238-016-0340-z
| 1 |
Aleckovic M, Kang Y (2015) Regulation of cancer metastasis by cellfree miRNAs. Biochim Biophys Acta 1855:24–42
|
| 2 |
Hawkins SM, Loomans HA, Wan YW, Ghosh-Choudhury T, Coffey D, Xiao W, Liu Z, Sangi-Haghpeykar H, Anderson ML (2013) Expression and functional pathway analysis of nuclear receptor NR2F2 in ovarian cancer. J Clin Endocrinol Metab 98:E1152–E1162
|
| 3 |
Huang CY, Chang AC, Chen HT, Wang SW, Lo YS, Tang CH (2016) Adiponectin promotes VEGF-C-dependent lymphangiogenesis by inhibiting miR-27b through CaMKII/AMPK/p38 signaling pathway in human chondrosarcoma cells. Clin Sci 130(17):1523–1533
|
| 4 |
Kang W, Tong JH, Chan AW, Zhao J, Dong Y, Wang S, Yang W, Sin FM, Ng SS, Yu J
|
| 5 |
Kieback DG, Levi T, Kohlberger P, Fiedrich U, Press MF, Rosenthal HE, Mobus VJ, Runnebaum IB, Tong XW, Tsai MJ (1996) Chicken ovalbumin upstream promoter–transcription factor (COUP-TF) expression in human endometrial cancer cell lines. Anticancer Res 16:3371–3376
|
| 6 |
Lee JJ, Drakaki A, Iliopoulos D, Struhl K (2012) MiR-27b targets PPARgamma to inhibit growth, tumor progression and the inflammatory response in neuroblastoma cells. Oncogene 31:3818–3825
|
| 7 |
Lin Y, Liu AY, Fan C, Zheng H, Li Y, Zhang C, Wu S, Yu D, Huang Z, Liu F
|
| 8 |
Liu AY, Cai Y, Mao Y, Lin Y, Zheng H, Wu T, Huang Y, Fang X, Lin S, Feng Q
|
| 9 |
Ma L, Teruya-Feldstein J, Weinberg RA (2007) Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature 449:682–688
|
| 10 |
Milne AN, Carneiro F, O’Morain C, Offerhaus GJ (2009) Nature meets nurture: molecular genetics of gastric cancer. Human Genet 126:615–628
|
| 11 |
Prahalad P, Dakshanamurthy S, Ressom H, Byers SW (2010) Retinoic acid mediates regulation of network formation by COUPTFII and VE-cadherin expression by TGFbeta receptor kinase in breast cancer cells. PloS ONE 5:e10023
|
| 12 |
Qin J, Chen X, Yu-Lee LY, Tsai MJ, Tsai SY (2010) Nuclear receptor COUP-TFII controls pancreatic islet tumor angiogenesis by regulating vascular endothelial growth factor/vascular endothelial growth factor receptor-2 signaling. Cancer Res 70:8812–8821
|
| 13 |
Qin J, Wu SP, Creighton CJ, Dai F, Xie X, Cheng CM, Frolov A, Ayala G, Lin X, Feng XH
|
| 14 |
Ruan K, Fang X, Ouyang G (2009) MicroRNAs: novel regulators in the hallmarks of human cancer. Cancer Lett 285:116–126
|
| 15 |
Shin SW, Kwon HC, Rho MS, Choi HJ, Kwak JY, Park JI (2009) Clinical significance of chicken ovalbumin upstream promotertranscription factor II expression in human colorectal cancer. Oncol Rep 21:101–106
|
| 16 |
Tao J, Zhi X, Zhang X, Fu M, Huang H, Fan Y, Guan W, Zou C (2015) miR-27b-3p suppresses cell proliferation through targeting receptor tyrosine kinase like orphan receptor 1 in gastric cancer. J Exp Clin Cancer Res 34:139
|
| 17 |
Tsai SY, Tsai MJ (1997) Chick ovalbumin upstreampromoter-transcription factors (COUP-TFs): coming of age. Endocr Rev 18:229–240
|
| 18 |
Yan Y, Wang LF, Wang RF (2015) Role of cancer-associated fibroblasts in invasion and metastasis of gastric cancer. World J Gastroenterol 21:9717–9726
|
| 19 |
Yao J, Deng B, Zheng L, Dou L, Guo Y, Guo K (2016) miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncol Rep 35:1645–1651
|
| 20 |
Zhang J, Ma L (2012) MicroRNA control of epithelial-mesenchymal transition and metastasis. Cancer Metastasis Rev 31:653–662
|
| 21 |
Zhang C, Han Y, Huang H, Qu L, Shou C (2014a) High NR2F2 transcript level is associated with increased survival and its expression inhibits TGF-beta-dependent epithelial-mesenchymal transition in breast cancer. Breast Cancer Res Treat 147:265–281
|
| 22 |
Zhang Y, Yang P, Wang XF (2014b) Microenvironmental regulation of cancer metastasis by miRNAs. Trends Cell Biol 24:153–160
|
| 23 |
Zheng L, Baumann U, Reymond JL (2004) An efficient one-step sitedirected and site-saturation mutagenesis protocol. Nucleic Acids Res 32:e115
|
| 24 |
Zhou B, Song J, Han T, Huang M, Jiang H, Qiao H, Shi J, Wang Y (2016) MiR-382 inhibits cell growth and invasion by targeting NR2F2 in colorectal cancer. Mol Carcinog.
|
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|
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