miR-27b inhibits gastric cancer metastasis by targeting NR2F2
Received date: 26 Jun 2016
Accepted date: 20 Oct 2016
Published date: 17 Mar 2017
Copyright
Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitroand in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells’ migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells’ proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients’ survival.
Key words: miR-27b; NR2F2; gastric cancer; tumor metastasis
Qingzhao Feng , Xionglin Wu , Fuchao Li , Beibei Ning , Xiaofeng Lu , Yin Zhang , Ying Pan , Wenxian Guan . miR-27b inhibits gastric cancer metastasis by targeting NR2F2[J]. Protein & Cell, 2017 , 8(2) : 114 -122 . DOI: 10.1007/s13238-016-0340-z
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