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Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs

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Published date: 13 Mar 2016

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2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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. Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs[J]. Protein & Cell, 2016 , 7(2) : 152 -156 . DOI: 10.1007/s13238-015-0228-3

1
Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka KF, Sakuma T, Yamamoto T, Tanaka K (2015) Cloning-freeCRISPR/Cas system facilitates functional cassette knock-in inmice. Genome Biol 16:87

DOI

2
Capecchi MR (2001) Generating mice with targeted mutations. NatMed 7:1086–1090

DOI

3
Chapman DL, Garvey N, Hancock S, Alexiou M, Agulnik SI, Gibson-Brown JJ, Cebra-Thomas J, Bollag RJ, Silver LM, Papaioannou VE (1996) Expression of the T-box family genes, Tbx1-Tbx5,during early mouse development. Dev Dyn. 206:379–390

DOI

4
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,Jiang W, Marraffini LA et al (2013) Multiplex genome engineeringusing CRISPR/Cas systems. Science 339:819–823

DOI

5
Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD (2013) High-frequency off-target mutagenesis induced byCRISPR-Cas nucleases in human cells. Nat Biotechnol 31:822–826

DOI

6
Hai T, Teng F, Guo R, Li W, Zhou Q (2014) One-step generation ofknockout pigs by zygote injection of CRISPR/Cas system. CellRes 24(3):372

DOI

7
Horsthuis T, Buermans HPJ, Brons JF, Verkerk AO, Bakker ML,Wakker V, Clout DEW, Moorman AFM, ‘t Hoen PAC, Christoffels VM (2009) Gene Expression Profiling of the Forming AtrioventricularNode Using a Novel Tbx3-Based Node-Specific TransgenicReporter. Circ Res 105:61–69

DOI

8
Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O et al (2013) DNA targetingspecificity of RNA-guided Cas9 nucleases. Nat Biotechnol 31:827–832

DOI

9
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE,Church GM (2013) RNA-guided human genome engineering viaCas9. Science 339:823–826

DOI

10
Niwa H, Ogawa K, Shimosato D, Adachi K (2009) A parallel circuit ofLIF signalling pathways maintains pluripotency of mouse EScells. Nature 460:118–122

DOI

11
Poueymirou WT, Auerbach W, Frendewey D, Hickey JF, Escaravage JM, Esau L, Dore AT, Stevens S, Adams NC, Dominguez MG et al (2007) F0 generation mice fully derived from gene-targetedembryonic stem cells allowing immediate phenotypic analyses. Nat Biotechnol 25:91–99

DOI

12
Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F,Jaenisch R (2013) One-step generation of mice carrying mutationsin multiple genes by CRISPR/Cas-mediated genomeengineering. Cell 153:910–918

DOI

13
Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013)One-step generation of mice carrying reporter and conditionalalleles by CRISPR/Cas-mediated genome engineering. Cell 154:1370–1379

DOI

14
Ying QL, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J,Cohen P, Smith A (2008) The ground state of embryonic stem cellself-renewal. Nature 453:519–523

DOI

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