MicroRNA-495 induces breast cancer cell migration by targeting JAM-A
Received date: 14 Apr 2014
Accepted date: 03 Jul 2014
Published date: 20 Nov 2014
Copyright
MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR (qRT-PCR). Junctional adhesion molecule A (JAM-A) was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-offunction assays, and the inhibition of JAM-A by miR-495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-495 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.
Key words： miR-495; JAM-A; breast cancer; migration
Minghui Cao , Weiwei Nie , Jing Li , Yujing Zhang , Xin Yan , Xiaoxiang Guan , Xi Chen , Ke Zen , Chen-yu Zhang , Xiaohong Jiang , Dongxia Hou . MicroRNA-495 induces breast cancer cell migration by targeting JAM-A[J]. Protein & Cell, 2014 , 5(11) : 862 -872 . DOI: 10.1007/s13238-014-0088-2
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