RESEARCH ARTICLE

MicroRNA-495 induces breast cancer cell migration by targeting JAM-A

  • Minghui Cao 1 ,
  • Weiwei Nie 2 ,
  • Jing Li 1 ,
  • Yujing Zhang 1 ,
  • Xin Yan 3 ,
  • Xiaoxiang Guan 2 ,
  • Xi Chen 1 ,
  • Ke Zen 1 ,
  • Chen-yu Zhang , 1 ,
  • Xiaohong Jiang , 1 ,
  • Dongxia Hou , 1
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  • 1. State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
  • 2. Department of Medical Oncology, Jinling Hospital, School of Medicine, Southern Medical University, Guangzhou 510282, China
  • 3. The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing 210008, China

Received date: 14 Apr 2014

Accepted date: 03 Jul 2014

Published date: 20 Nov 2014

Copyright

2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR (qRT-PCR). Junctional adhesion molecule A (JAM-A) was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-offunction assays, and the inhibition of JAM-A by miR-495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-495 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.

Cite this article

Minghui Cao , Weiwei Nie , Jing Li , Yujing Zhang , Xin Yan , Xiaoxiang Guan , Xi Chen , Ke Zen , Chen-yu Zhang , Xiaohong Jiang , Dongxia Hou . MicroRNA-495 induces breast cancer cell migration by targeting JAM-A[J]. Protein & Cell, 2014 , 5(11) : 862 -872 . DOI: 10.1007/s13238-014-0088-2

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