An engineered xCas12i with high activity, high specificity, and broad PAM range
Hainan Zhang, Xiangfeng Kong, Mingxing Xue, Jing Hu, Zikang Wang, Yinghui Wei, Haoqiang Wang, Jingxing Zhou, Weihong Zhang, Mengqiu Xu, Xiaowen Shen, Fengcai Yin, Zhiyuan Ai, Guangyan Huang, Junhui Xia, Xueqiong Song, Hengbin Li, Yuan Yuan, Jinhui Li, Na Zhong, Meiling Zhang, Yingsi Zhou, Hui Yang
An engineered xCas12i with high activity, high specificity, and broad PAM range
[1] |
Anzalone AV, Koblan LW, Liu DR. Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 2020;38:824–844.
CrossRef
Google scholar
|
[2] |
Bae S, Park J, Kim JS. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 2014;30:1473–1475.
CrossRef
Google scholar
|
[3] |
Bock C, Datlinger P, Chardon F et al. High-content CRISPR screening. Nat Rev Methods Primers 2022;2:9.
CrossRef
Google scholar
|
[4] |
Bravo JPK, Liu M-S, Hibshman GN et al. Structural basis for mismatch surveillance by CRISPR-Cas9. Nature 2022;603:343–347.
CrossRef
Google scholar
|
[5] |
Chen Y, Hu Y, Wang X et al. Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing. Innovation (Camb) 2022;3:100264.
CrossRef
Google scholar
|
[6] |
Doudna JA. The promise and challenge of therapeutic genome editing. Nature 2020;578:229–236.
CrossRef
Google scholar
|
[7] |
Gillmore JD, Gane E, Taubel J et al. CRISPR-Cas9 in vivo gene editing for transthyretin amyloidosis. N Engl J Med 2021;385: 493–502.
CrossRef
Google scholar
|
[8] |
Kleinstiver BP, Sousa AA, Walton RT et al. Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol 2019;37:276–282.
CrossRef
Google scholar
|
[9] |
McGaw C, Garrity AJ, Munoz GZ et al. Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editing. Nat Commun 2022;13:2833.
CrossRef
Google scholar
|
[10] |
Richter MF, Zhao KT, Eton E et al. Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol 2020;38:883–891.
CrossRef
Google scholar
|
[11] |
Yan WX, Hunnewell P, Alfonse LE et al. Functionally diverse type V CRISPR-Cas systems. Science 2019;363:88–91.
CrossRef
Google scholar
|
[12] |
Yin J, Liu M, Liu Y et al. Optimizing genome editing strategy by primer-extension-mediated sequencing. Cell Discov 2019;5:18.
CrossRef
Google scholar
|
[13] |
Wang X, Ding C, Yu W et al. Cas12a base editors induce efficient and specific editing with low DNA damage response. Cell Rep 2020;31:107723.
CrossRef
Google scholar
|
[14] |
Zetsche B, Gootenberg JS, Abudayyeh OO et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell 2015;163:759–771.
CrossRef
Google scholar
|
[15] |
Zhang, Zuris JA, Viswanathan R et al. AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines. Nat Commun 2021;12:3908.
CrossRef
Google scholar
|
/
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