The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins

Xi Zhang, Yongqiang Deng, Hao Chang, Chen Ji, Mingshu Zhang, Jianxin Peng, Tao Xu, Pingyong Xu

PDF(489 KB)
PDF(489 KB)
Protein Cell ›› 2014, Vol. 5 ›› Issue (10) : 800-803. DOI: 10.1007/s13238-014-0090-8
LETTER
LETTER

The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins

Author information +
History +

Cite this article

Download citation ▾
Xi Zhang, Yongqiang Deng, Hao Chang, Chen Ji, Mingshu Zhang, Jianxin Peng, Tao Xu, Pingyong Xu. The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins. Protein Cell, 2014, 5(10): 800‒803 https://doi.org/10.1007/s13238-014-0090-8

References

[1]
Banaszynski LA, Chen L-C, Maynard-Smith LA, Ooi AGL, Wandless TJ (2006) A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell126: 995-1004
CrossRef Google scholar
[2]
Chen I, Howarth M, Lin W, Ting AY (2005) Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nat Methods2: 99-104
CrossRef Google scholar
[3]
Dittman J, Ryan TA (2009) Molecular circuitry of endocytosis at nerve terminals. Annu Rev Cell Dev Biol25: 133-160
CrossRef Google scholar
[4]
Eliasson L, Abdulkader F, Braun M, Galvanovskis J, Hoppa MB, Rorsman P (2008) Novel aspects of the molecular mechanisms controlling insulin secretion. J Physiol586: 3313-3324
CrossRef Google scholar
[5]
George N, Pick H, Vogel H, Johnsson N, Johnsson K (2004) Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc126: 8896-8897
CrossRef Google scholar
[6]
Grote E, Kelly RB (1996) Endocytosis of VAMP is facilitated by a synaptic vesicle targeting signal. J Cell Biol132: 537-547
CrossRef Google scholar
[7]
Harel A, Wu F, Mattson MP, Morris CM, Yao PJ (2008) Evidence for CALM in directing VAMP2 trafficking. Traffic9: 417-429
CrossRef Google scholar
[8]
Hoffmann C, Gaietta G, Zurn A, Adams SR, Terrillon S, Ellisman MH, Tsien RY, Lohse MJ (2010) Fluorescent labeling of tetracysteinetagged proteins in intact cells. Nat Protoc5: 1666-1677
CrossRef Google scholar
[9]
Keppler A, Gendreizig S, Gronemeyer T, Pick H, Vogel H, Johnsson K (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotech21: 86-89
CrossRef Google scholar
[10]
Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, Wood MG, Learish R, Ohana RF, Urh M (2008) HaloTag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem Biol3: 373-382
CrossRef Google scholar
[11]
Miller SE, Sahlender DA, Graham SC, Höning S, Robinson MS, Peden AA, Owen DJ (2011) The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Cell147: 1118-1131
CrossRef Google scholar
[12]
Regazzi R, Wollheim C, Lang J, Theler J, Rossetto O, Montecucco C, Sadoul K, Weller U, Palmer M, Thorens B (1995) VAMP-2 and cellubrevin are expressed in pancreatic beta-cells and are essential for Ca(2+)-but not for GTP gamma S-induced insulin secretion. EMBO J14: 2723
[13]
Sudhof TC (2004) The synaptic vesicle cycle. Annu Rev Neurosci27: 509-547
CrossRef Google scholar
[14]
Sun X, Zhang A, Baker B, Sun L, Howard A, Buswell J, Maurel D, Masharina A, Johnsson K, Noren CJ (2011) Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging. ChemBioChem12: 2217-2226
CrossRef Google scholar
[15]
Uttamapinant C, White KA, Baruah H, Thompson S, Fernández-Suárez M, Puthenveetil S, Ting AY (2010) A fluorophore ligase for site-specific protein labeling inside living cells. Proc Natl Academy Sci107: 10914-10919
CrossRef Google scholar

RIGHTS & PERMISSIONS

2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
AI Summary AI Mindmap
PDF(489 KB)

Accesses

Citations

Detail

Sections
Recommended

/