Fiber-coupler-based microfluidic system for trapping of DNA biomolecules

Abstract

A miniature fiber-coupler-based microfluidic system is proposed for trapping of DNA biomolecules. The loop-shaped fiber-coupler is fabricated by using flame tapering technique and integrated in a microfluidic channel. Probe-DNA immobilized on the fiber-coupler surface enables specific recognition of target DNA sequences and effectively facilitates the trapping of target DNA molecules. The binding characteristics of biomolecules on the fiber-coupler surface have been theoretically analyzed and experimentally demonstrated. Experimental results indicate that the spectral response of the loop-shaped fiber coupler immobilized with probe DNA exhibits a red-shift with the trapping of the DNA biomolecules. The proposed microfluidic system possesses such desirable merits as simple structure, label-free method and high integration, which make it a promising candidate for applications in molecular biology and related bioengineering areas.

References

[1] KangT, YooS M, YoonI, LeeS Y, KimB. Nano Lett., 2010, 10: 1189

[2] FlissM S, UsadelH, CaballeroO L, WuL, ButaM R, EleffS M, JenJ, SidranskyD. Science, 2000, 287: 2017

[3] LeeJ, CheongK H, HuhN, KimS, ChoiJ, KoC. Lab Chip, 2006, 6: 886

[4] HarrisT D, BuzbyP R, BabcockH, BeerE, BowersJ, BraslavskyI, CauseyM, ColonellJ, DiMeoJ, EfcavitchJ W, GiladiE, GillJ, HealyJ, JaroszM, LapenD, MoultonK, QuakeS R, SteinmannK, ThayerE, TyurinaA, WardR, WeissH, XieZ. Science, 2008, 320: 106

[5] AhnS, WaltD R. Anal. Chem., 2005, 77: 5041

[6] FergusonJ A, SteemersF J, WaltD R. Anal. Chem., 2000, 72: 5618

[7] WangJ. Anal. Chem. Acta, 2003, 500: 247

[8] CaiH, CaoX, JiangY, HeP, FangY. Anal. Bioanal. Chem., 2003, 375: 287

[9] ChenH, WeiJ, PanJ, ZhangW, DangF, ZhangZ, ZhangJ. Biosens. Bioelectron., 2017, 91: 328

[10] ZhaoX, DytiocoR T, TanW. J. Am. Chem. Soc., 2003, 125: 11474

[11] DaemsD, KnezK, DelportF, SpasicD, LammertynJ. Analyst, 2016, 141: 1906

[12] SatoK, TachiharaA, RenbergB, MawatariK, SatoK, TanakaY, JarviusJ, NilssonM, KitamoriT. Lab Chip, 2010, 10: 1262

[13] LiuY, ChenS, LiuQ, PengW. Opt. Exp., 2015, 23: 20686

[14] PanichS, SleimanM H, SteerI, LadameS, EdelJ B. ACS Sens, 2016, 1: 1097

[15] SongB, ZhangH, LiuB, LinW, WuJ. Biosens. Bioelectron., 2016, 81: 151

[16] JangH S, ParkK N, KimJ P, SimS J, KwonO J, HanY G, LeeK S. Opt. Exp., 2009, 17: 3855

[17] SunD, GuoT, RanY, HuangY, GuanB. Biosens. Bioelectron., 2014, 61: 541

[18] GrattanK T V, SunD T. Sens. Actuators A, Phys., 2000, 82: 40

[19] TazawaH, KanieT, KatayamaM. Appl. Phys. Lett., 2007, 91: 113901

[20] LinW, ZhangH, SongB, MiaoY, LiuB, YanD, LiuY. Opt. Exp., 2015, 23: 11123

[21] MaoL, PuS, SuD, WangZ, ZengX, LahoubiM. J. Appl. Phys., 2016, 120: 093102

[22] DingM, WangP, BrambillaG. Opt. Exp., 2012, 20: 5402

[23] PuS, LuoL, TangJ, MaoL, ZengX. IEEE Photon. Technol. Lett., 2016, 28: 1073

[24] ChenY, YanS, ZhengX, XuF, LuY. Opt. Exp., 2014, 22: 2443

[25] TengJ, YangJ, LvC, ChenT, GuoJ, FengJ, WuP. Opt. Fiber Technol., 2004, 20: 239

[26] HeZ, TianF, ZhuY, LavlinskaiaN, DuH. Biosens. Bioelectron., 2011, 26: 4774

[27] LiuY, ChenS, LiuQ, PengW. Opt. Exp., 2015, 23: 20686

[28] LevickyR, HorganA. Trends Biotechnol., 2005, 23: 143