Time-resolved two-photon excitation fluorescence spectroscopy and microscopy using a high repetition rate streak camera

Li-xin Liu; Ju-le Qu; Zi-yang Lin; Lei Wang; Zhe Fu; Bao-ping Guo; Han-ben Niu

doi:10.1007/s11801-007-7019-x

Optoelectronics Letters ›› 2007, Vol. 3 ›› Issue (2) : 157-160. DOI: 10.1007/s11801-007-7019-x

Article

Time-resolved two-photon excitation fluorescence spectroscopy and microscopy using a high repetition rate streak camera

Author information +

History +

Abstract

We present a time-resolved two-photon excitation fluorescence spectroscopy and a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy system that is based on a high repetition rate picosecond streak camera for providing time-and spectrum-resolved measurement and imaging in biomedicine. The performance of the system is tested and characterized by the fluorescence spectrum and lifetime analysis of several standard fluorescent dyes and their mixtures. Spectrum-resolved fluorescence lifetime images of fluorescence beads are obtained. Potential applications of the system include clinical diagnostics and cell biology etc.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download citation ▾

Li-xin Liu, Ju-le Qu, Zi-yang Lin, Lei Wang, Zhe Fu, Bao-ping Guo, Han-ben Niu. Time-resolved two-photon excitation fluorescence spectroscopy and microscopy using a high repetition rate streak camera. Optoelectronics Letters, 2007, 3(2): 157‒160 https://doi.org/10.1007/s11801-007-7019-x

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	DenkW., StricklerJ.H., WebbW.W.. Science, 1990, 248: 73 CrossRef Google scholar

[2]	HiraokaY., ShimiT., HaraguchiT.. Cell Struc. Func., 2002, 27: 367 CrossRef Google scholar

[3]	MarcuL., JoJ. A., ButteP. V., YoungW. H., PikulB. K., BlackK. L., ThompsonR. C.. Photochem. Photobiol., 2004, 80: 98 CrossRef Google scholar

[4]	AshjianP., ElbarbaryA., ZukP.. Tissue Eng., 2004, 10: 411 CrossRef Google scholar

[5]	BewersdorfJ., PickR., HellS.W.. Opt. Lett., 1998, 23: 655

[6]	BuistA.H., MüllerM., squireJ., BrakenhoffG.J.. J. Microsc., 1998, 192: 217 CrossRef Google scholar

[7]	SacconiL., FronerE., AntoliniR., TaghizadehM.R., ChoudhuryA., PavoneF. S.. Opt. Lett., 2003, 28: 1918

[8]	StraubM., HellS.W.. Appl. Phys. Lett., 1998, 73: 1769 CrossRef Google scholar

[9]	Lévêque-FortS., PenonJ., Fontaine-AupartM. P., RogerG., GeorgesP.. Proc. SPIE, 2003, 5139: 173 CrossRef Google scholar

[10]	SiegelJ., ElsonD.S., WebbS.E.D., Parsons-KaravassilisD., Lévêque-FortS., ColeM.J., LeverM.J., FrenchP.M.W., NeilM.A.A., JuskaitisR., SucharovL.O., WilsonT.. Opt. Lett., 2001, 26: 1338

[11]	BeckerW., BergmannA., BiskupC., ZimmerT., KlöckerN., BenndorfK.. Proc. SPIE, 2002, 4620: 79 CrossRef Google scholar

[12]	GlanzmannT., BalliniJ. P., van den BerghH., WagnièresG.. Rev. Sci. Instrum., 1999, 70: 4067 CrossRef Google scholar

[13]	BiskupC., ZimmerT., BenndorfK.. Nat. Biotech., 2004, 22: 220 CrossRef Google scholar

[14]	KrishnanR. V., SaitohH., TeradaH., CentonzeV. E., HermanB.. Rev. Sci. Instrum., 2003, 74: 2714 CrossRef Google scholar

[15]	LiuL., QuJ., LinZ., ChenD., HuT., GuoB., NiuH.. Acta Opt. Sin., 2006, 26: 373

[16]	LiuL., QuJ., LinZ., ChenD., XuG., HuT., GuoB., NiuH.. Acta Phys. Sin., 2006, 55: 6281

[17]	QuJ., LiuL., ChenD., LinZ., XuG., GuoB., NiuH.. Opt. Lett., 2006, 31: 368 CrossRef Google scholar

[18]	LinZ., FuZ., LiuL., HuT., QuJ., GuoB., NiuH.. Acta Phys. Sin., 2006, 55: 6701

[19]	U. Brackmann, Lambdachrome Laser Dyes Lambda Physic AG, Goettingen, 2000.

[20]	ColeM.J., SiegelJ., WebbS.E.D., JonesR., DowlingK., DayelM.J., Parsons-KaravassilisD., FrenchP.M.W., LeverM.J., SucharovL.O.D., NeilM.A.A., JuškaitisR., WilsonT.. J. microsc., 2001, 203: 246 CrossRef Google scholar