Conditions of synthesis for PpIX

Abstract

The characteristics of ALA absorption spectra under different conditions are studied with spectrometers. The results indicate that the wavelength of ALA, corresponding to the strongest absorption peak, is 282 nm under different conditions. The content of PpIX synthesized is in direct proportion to the density of Jurkat cells. The most suitable culture time is 8 hours in order to synthesize more PpIX. FCS has a block effect on the synthesis of PpIX.

References

[1] KonanY. N., GurnyR., AllemannE.. J Photochem Photobiol B. Biol, 2002, 66: 89-89

[2] BonnettR.. Chem Soc Rev, 1995, 24: 19-19

[3] TarakD. M.. J Porphyrins Phthalocyanines, 2000, 4: 362-362

[4] XiongJ., XiaoH., ChenL.. J. of Optoelectronics · Laser, 2004, 15: 1118-1118(in Chinese)

[5] BartosovaJ., HrkalZ.. Comparative Biochemistry and Physiology Part C, 2000, 126: 245-245

[6] EvensenJ. F.. Acta Oncologica, 1995, 34: 1103-1103

[7] PengQ.. Cancer, 1997, 179: 2282-2282

[8] FriesenS. A., HjortlandG. O.. International Journal of Oncology, 2002, 21: 577-577

[9] LingX., LiW.. Spectroscopy and Spectral Analysis, 2000, 20: 692-692

[10] ShengJ., LiS., ChenZ.. Chin J Laser Med Surg, 2001, 10: 145-145

[11] ZhengH., ZhangJ., ZhuG.. Chinese Journal of Spectroscopy Laboratory, 2003, 20: 941-941

[12] HeH., ShuY.. Chinese Journal of Analysis Laboratory, 2003, 20: 101-101

[13] SibataC. H., ColussiV. C., OleinickN. L.. Braz. J Med Bio Res, 2000, 33: 869-869

[14] ColussiV. C., FeyesD. K., MukhtaH.. Skin Pharmacology and Applied Skin Physiology, 1998, 11: 336-336

[15] MaierA., TomaselliF., MatziV.. Lasers in Surgery and Medicine, 2001, 29: 323-323