A tool to assess the mock community samples in 16S rRNA gene-based microbiota profiling studies

Abstract

Inclusion and investigation of technical controls in microbiome sequencing studies is important for understanding technical biases and errors. Here, we present chkMocks, a general R-based tool that allows researchers to compare the composition of mock communities that are processed along with samples to their theoretical composition. A visual comparison between experimental and theoretical community composition and their correlation is provided for researchers to assess the quality of their sample processing workflows.

Keywords

Mock community / microbiome profiling / positive control

References

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