Not too rigid nor too wobbly: Defining an optimal membrane fluidity range essential for biofilm formation in Escherichia coli

References

[1] Sauer K, Stoodley P, Goeres DM, Hall-Stoodley L, Burmølle M, Stewart PS, et al. The biofilm life cycle: expanding the conceptual model of biofilm formation. Nat Rev Microbiol. 2022; 20: 608-620.

[2] Cronan JE, Rock CO. Biosynthesis of membrane lipids. EcoSal Plus. 2008; 3: ecosalplus.3.6.4.

[3] Dubois-Brissonnet F, Trotier E, Briandet R. The biofilm lifestyle involves an increase in bacterial membrane saturated fatty acids. Front Microbiol. 2016; 7: 1673.

[4] Nepper JF, Lin YC, Weibel DB. Rcs phosphorelay activation in cardiolipin-deficient Escherichia coli reduces biofilm formation. J Bacteriol. 2019; 201: e00804-18.

[5] Campbell JW, Cronan JE Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery. Annu Rev Microbiol. 2001; 55: 305-332.

[6] Yao J, Rock CO. Bacterial fatty acid metabolism in modern antibiotic discovery. Biochim Biophys Acta Mol Cell Biol Lipids. 2017; 1862: 1300-1309.

[7] Hong Y, Qin J, Verderosa AD, Hawas S, Zhang B, Blaskovich MAT, et al. Loss of β-ketoacyl acyl carrier protein synthase III activity restores multidrug-resistant Escherichia coli sensitivity to previously ineffective antibiotics. mSphere. 2022; 7: e0011722.

[8] Guo Q, Su J, Liao Y, Yu Y, Luo L, Weng X, et al. An atypical 3-ketoacyl ACP synthase III required for acyl homoserine lactone synthesis in Pseudomonas syringae pv. syringae B728a. Appl Environ Microbiol. 2024; 90: e02256-02223.

[9] Marr AG, Ingraham JL. Effect of temperature on the composition of fatty acids in Escherichia coli. J Bacteriol. 1962; 84: 1260-1267.

[10] Goelz SE, Cronan JE. The positional distribution of fatty acids in Escherichia coli phospholipids is not regulated by sn-glycerol 3-phosphate levels. J Bacteriol. 1980; 144: 462-464.

[11] Gelmann EP, Cronan JE Mutant of Escherichia coli deficient in the synthesis of cis-vaccenic acid. J Bacteriol. 1972; 112: 381-387.

[12] Garwin JL, Klages AL, Cronan JE. Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis. J Biol Chem. 1980; 255: 3263-3265.

[13] Feng Y, Cronan JE. Escherichia coli unsaturated fatty acid synthesis. J Biol Chem. 2009; 284: 29526-29535.

[14] Dong H, Cronan JE. Temperature regulation of membrane composition in the Firmicute, Enterococcus faecalis, parallels that of Escherichia coli. Environ Microbiol. 2021; 23: 2683-2691.

[15] Serra DO, Richter AM, Hengge R. Cellulose as an architectural element in spatially structured Escherichia coli biofilms. J Bacteriol. 2013; 195: 5540-5554.

[16] Boggs JM. Lipid intermolecular hydrogen bonding: influence on structural organization and membrane function. Biochim Biophys Acta Rev Biomembr. 1987; 906: 353-404.

[17] Dawaliby R, Trubbia C, Delporte C, Noyon C, Ruysschaert JM, Van Antwerpen P, et al. Phosphatidylethanolamine is a key regulator of membrane fluidity in eukaryotic cells. J Biol Chem. 2016; 291: 3658-3667.

[18] Klose C, Surma MA, Gerl MJ, Meyenhofer F, Shevchenko A, Simons K. Flexibility of a eukaryotic lipidome--insights from yeast lipidomics. PLoS One. 2012; 7: e35063.

[19] Price AC, Choi KH, Heath RJ, Li Z, White SW, Rock CO. Inhibition of β-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. J Biol Chem. 2001; 276: 6551-6559.

[20] Dramé I, Rossez Y, Krzewinski F, Charbonnel N, Ollivier-Nakusi L, Briandet R, et al. FabR, a regulator of membrane lipid homeostasis, is involved in Klebsiella pneumoniae biofilm robustness. mBio. 2024; 15: e01317-e01324.

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