Unwinding matters: WED domain determines Cas9 activity by accelerating DNA unwinding and R-loop formation

Mei Luo; Shaohua Yao

doi:10.1002/mba2.107

MEDCOMM - Biomaterials and Applications ›› 2024, Vol. 3 ›› Issue (4) : e107 DOI: 10.1002/mba2.107

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Unwinding matters: WED domain determines Cas9 activity by accelerating DNA unwinding and R-loop formation

Mei Luo
, Shaohua Yao ^†

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Mei Luo, Shaohua Yao. Unwinding matters: WED domain determines Cas9 activity by accelerating DNA unwinding and R-loop formation. MEDCOMM - Biomaterials and Applications, 2024, 3(4): e107 DOI:10.1002/mba2.107

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References

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[1]	EggersAR, ChenK, SoczekKM, et al. Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9. Cell. 2024;187(13):3249-3261.

[2]	GongS, YuHH, JohnsonKA, Taylor DW. DNA unwinding is the primary determinant of CRISPR-Cas9 activity. Cell Rep. 2018;22(2):359-371.

[3]	ChenK, HanH, ZhaoS, et al. Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP. bioRxiv. 2023. 2023.11.15.566339.

[4]	HuJH, MillerSM, GeurtsMH, et al. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018;556(7699):57-63.

[5]	MaE, Harrington LB, O’ConnellMR, ZhouK, DoudnaJA. Single-stranded DNA cleavage by divergent CRISPR-Cas9 enzymes. Mol Cell. 2015;60(3):398-407.

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2024 The Author(s). MedComm - Biomaterials and Applications published by John Wiley & Sons Australia, Ltd on behalf of Sichuan International Medical Exchange & Promotion Association (SCIMEA).