Antimicrobial Effect of Spices and Their Phytochemicals: A Novel Approach to Overcoming Antibiotic Resistance

Abstract

Antibiotic resistance is a significant global health challenge that demands innovative strategies to combat resistant pathogens. Spices, known for their culinary and medicinal qualities, have emerged as promising sources of antimicrobial agents due to their rich content of potent bioactive phytochemicals. Compounds such as flavonoids, phenolics, alkaloids, and terpenoids exhibit strong antibacterial, antifungal, and antiviral activities. These phytochemicals target microbial cell walls, membranes, and metabolic processes, effectively inhibiting pathogen growth and survival. Additionally, their ability to disrupt biofilms and synergize with conventional antibiotics enhances their potential to counter resistance mechanisms. This review examines the mechanisms and dissemination of antimicrobial resistance, the antimicrobial properties of spices and their phytochemicals, focusing on their modes of action, efficacy against multidrug-resistant pathogens, specific extraction methods for each phytochemical, synergism with traditional antibiotics, safety and toxicological concerns, future research directions, and challenges in the widespread use of these spice-derived compounds. It highlights the vast array of antimicrobial solutions derived from these spices and their natural phytochemicals, offering sustainable and effective means to address the escalating threat of antibiotic resistance.

Keywords

antimicrobial action / antibiotic resistance / bioactive / extraction processes / phytochemicals / spices

References

[1] M. A. Salam, M. Y. Al-Amin, M. T. Salam, et al., “Antimicrobial Resistance: A Growing Serious Threat for Global Public Health,” Healthcare 11 (2023): 1946.

[2] C. S. Ho, C. T. H. Wong, T. T. Aung, et al., “Antimicrobial Resistance: A Concise Update,” The Lancet Microbe 6 (2024): 100947.

[3] L. Diniz do Nascimento, A. A. B. de Moraes, K. S. da Costa, et al., “Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications,” Biomolecules 10 (2020): 988.

[4] P. Khatri, A. Rani, S. Hameed, S. Chandra, C. M. Chang, and R. P. Pandey, “Current Understanding of the Molecular Basis of Spices for the Development of Potential Antimicrobial Medicine,” Antibiotics 12 (2023): 270.

[5] K. E. Vivekanandan, P. V. Kumar, R. C. Jaysree, and T. Rajeshwari, “Exploring Molecular Mechanisms of Drug Resistance in Bacteria and Progressions in CRISPR/Cas9-Based Genome Expurgation Solutions,” Global Medical Genetics 12, no. 2 (2025): 100042.

[6] K. Bush and P. A. Bradford, “β-Lactams and β-Lactamase Inhibitors: An Overview,” Cold Spring Harbor Perspectives in Medicine 6 (2016): a025247.

[7] C. L. Tooke, P. Hinchliffe, E. C. Bragginton, et al., “β-Lactamases and β-Lactamase Inhibitors in the 21st Century,” Journal of Molecular Biology 431 (2019): 3472–3500.

[8] G. Alvisi, A. Curtoni, R. Fonnesu, et al., “Epidemiology and Genetic Traits of Carbapenemase-Producing Enterobacterales: A Global Threat to Human Health,” Antibiotics 14 (2025): 141–141.

[9] M. Mora-Ochomogo and C. T. Lohans, “β-Lactam Antibiotic Targets and Resistance Mechanisms: From Covalent Inhibitors to Substrates,” RSC Medicinal Chemistry 12 (2021): 1623–1639.

[10] D. Brdová, T. Ruml, and J. Viktorová, “Mechanism of Staphylococcal Resistance to Clinically Relevant Antibiotics,” Drug Resistance Updates 77 (2024): 101147–101147.

[11] C. Sibold, J. Henrichsen, A. König, C. Martin, L. Chalkley, and R. Hakenbeck, “MosaicpbpXgenes of Major Clones of Penicillin-resistant Streptococcuspneumoniaehave Evolved frompbpXgenes of a Penicillin-Sensitive Streptococcus Oralis,” Molecular Microbiology 12 (1994): 1013–1023.

[12] D. Zeng, D. Debabov, T. L. Hartsell, et al., “Approved Glycopeptide Antibacterial Drugs: Mechanism of Action and Resistance,” Cold Spring Harbor Perspectives in Medicine 6 (2016): a026989.

[13] J. Xie, J. G. Pierce, R. C. James, A. Okano, and D. L. Boger, “A Redesigned Vancomycin Engineered for Duald-Ala-d-Ala andd-Ala-d-Lac Binding Exhibits Potent Antimicrobial Activity Against Vancomycin-Resistant Bacteria,” Journal of the American Chemical Society 133 (2011): 13946–13949.

[14] C. M. Hill, K. M. Krause, S. R. Lewis, et al., “Specificity of Induction of the vanA and vanB Operons in Vancomycin-Resistant Enterococci by Telavancin,” Antimicrobial Agents and Chemotherapy 54 (2010): 2814–2818.

[15] K. M. Krause, A. W. Serio, T. R. Kane, and L. E. Connolly, “Aminoglycosides: An Overview,” Cold Spring Harbor Perspectives in Medicine 6 (2016): a027029.

[16] M. S. Ramirez, N. Nikolaidis, and M. E. Tolmasky, “Rise and Dissemination of Aminoglycoside Resistance: The Aac(6′)-Ib Paradigm,” Frontiers in Microbiology 4 (2013): 121.

[17] M. S. Ramirez and M. E. Tolmasky, “Aminoglycoside Modifying Enzymes,” Drug Resistance Updates 13 (2010): 151–171.

[18] S. R. Connell, D. M. Tracz, K. H. Nierhaus, and D. E. Taylor, “Ribosomal Protection Proteins and Their Mechanism of Tetracycline Resistance,” Antimicrobial Agents and Chemotherapy 47 (2003): 3675–3681.

[19] T. Tenson, M. Lovmar, and M. Ehrenberg, “The Mechanism of Action of Macrolides, Lincosamides and Streptogramin B Reveals the Nascent Peptide Exit Path in the Ribosome,” Journal of Molecular Biology 330 (2003): 1005–1014.

[20] M. S. Svetlov, E. A. Syroegin, E. V. Aleksandrova, et al., “Structure of Erm-modified 70S Ribosome Reveals the Mechanism of Macrolide Resistance,” Nature Chemical Biology 17 (2021): 412–420.

[21] K. S. Long and B. Vester, “Resistance to Linezolid Caused by Modifications at Its Binding Site on the Ribosome,” Antimicrobial Agents and Chemotherapy 56 (2011): 603–612.

[22] P. Gupta, S. Sothiselvam, N. Vázquez-Laslop, and A. S. Mankin, “Deregulation of Translation due to Post-transcriptional Modification of rRNA Explains Why Erm Genes Are Inducible,” Nature Communications 4 (2013): 1984.

[23] H. Chen, W. Wu, M. Ni, et al., “Linezolid-resistant Clinical Isolates of Enterococci and Staphylococcus Cohnii from a Multicentre Study in China: Molecular Epidemiology and Resistance Mechanisms,” International Journal of Antimicrobial Agents 42 (2013): 317–321.

[24] D. C. Hooper and G. A. Jacoby, “Topoisomerase Inhibitors: Fluoroquinolone Mechanisms of Action and Resistance,” Cold Spring Harbor Perspectives in Medicine 6 (2016): a025320.

[25] G. A. Jacoby, J. Strahilevitz, and D. C. Hooper, “Plasmid-Mediated Quinolone Resistance,” Microbiology Spectrum 2 (2014), https://doi.org/10.1128/microbiolspec.plas-0006-2013.

[26] M. Li, L. J. Chao, Y. Lu, et al., “rpoB Mutations and Effects on Rifampin Resistance in Mycobacterium Tuberculosis,” Infection and Drug Resistance 14 (2021): 4119–4128.

[27] M. Kiani, A. Astani, G. Eslami, et al., “Upstream Region of OprD Mutations in Imipenem-resistant and Imipenem-sensitive Pseudomonas Isolates,” AMB Express 11 (2021): 82.

[28] C. Bornet, N. Saint, L. Fetnaci, et al., “Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins,” Antimicrobial Agents and Chemotherapy 48 (2004): 2153–2158.

[29] M. Masi, J. Vergalli, I. Ghai, et al., “Cephalosporin Translocation across Enterobacterial OmpF and OmpC Channels, a Filter across the Outer Membrane,” Communications Biology 5 (2022): 1059.

[30] J. Anes, M. P. McCusker, S. Fanning, and M. Martins, “The Ins and Outs of RND Efflux Pumps in Escherichia coli,” Frontiers in Microbiology 6 (2015): 587.

[31] E. W. Yu, J. R. Aires, and H. Nikaido, “AcrB Multidrug Efflux Pump of Escherichia coli: Composite Substrate-Binding Cavity of Exceptional Flexibility Generates Its Extremely Wide Substrate Specificity,” Journal of Bacteriology 185 (2003): 5657–5664.

[32] A. B. Lorusso, J. A. Carrara, C. D. N. Barroso, F. F. Tuon, and H. Faoro, “Role of Efflux Pumps on Antimicrobial Resistance in Pseudomonas aeruginosa,” International Journal of Molecular Sciences 23 (2022): 15779.

[33] M. Sánchez-Osuna, P. Cortés, J. Barbé, and I. Erill, “Origin of the Mobile Di-Hydro-Pteroate Synthase Gene Determining Sulfonamide Resistance in Clinical Isolates,” Frontiers in Microbiology 9 (2019): 3332.

[34] S. M. Reeve, D. Si, J. Krucinska, et al., “Toward Broad Spectrum Dihydrofolate Reductase Inhibitors Targeting Trimethoprim Resistant Enzymes Identified in Clinical Isolates of Methicillin Resistant Staphylococcus aureus,” ACS Infectious Diseases 5 (2019): 1896–1906.

[35] M. Venkatesan, M. Fruci, L. A. Verellen, et al., “Molecular Mechanism of Plasmid-borne Resistance to Sulfonamide Antibiotics,” Nature Communications 14 (2023): 4031.

[36] F. F. Andrade, D. Silva, A. Rodrigues, and C. Pina-Vaz, “Colistin Update on Its Mechanism of Action and Resistance, Present and Future Challenges,” Microorganisms 8 (2020): 1716.

[37] D. Lakshmanan, D. Ramasamy, V. Subramanyam, and S. Suresh, “Mobile Colistin Resistance (mcr) Genes and Recent Developments in Colistin Resistance Detection,” Letters in Applied Microbiology 76 (2023): ovad102.

[38] P. Hinchliffe, Q. E. Yang, E. Portal, et al., “Insights into the Mechanistic Basis of Plasmid-Mediated Colistin Resistance from Crystal Structures of the Catalytic Domain of MCR-1,” Scientific Reports 7 (2017): 39392.

[39] A. M. S. Shein, D. L. Wannigama, P. G. Higgins, et al., “High Prevalence of mgrB-mediated Colistin Resistance among Carbapenem-resistant Klebsiella pneumoniae Is Associated with Biofilm Formation, and Can be Overcome by Colistin-EDTA Combination Therapy,” Scientific Reports 12 (2022): 12939.

[40] H. Y. Liu, E. L. Prentice, and M. A. Webber, “Mechanisms of Antimicrobial Resistance in Biofilms,” Npj Antimicrobials and Resistance 2 (2024): 27.

[41] S. Sharma, J. Mohler, S. D. Mahajan, S. A. Schwartz, L. Bruggemann, and R. Aalinkeel, “Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment,” Microorganisms 11 (2023): 1614.

[42] L. R. Mulcahy, J. L. Burns, S. Lory, and K. Lewis, “Emergence of Pseudomonas aeruginosa Strains Producing High Levels of Persister Cells in Patients with Cystic Fibrosis,” Journal of Bacteriology 192 (2010): 6191–6199.

[43] S. Yang, I. D. Hay, D. R. Cameron, et al., “Antibiotic Regimen Based on Population Analysis of Residing Persister Cells Eradicates Staphylococcus epidermidis Biofilms,” Scientific Reports 5 (2015): 18578.

[44] K. W. K. Tang, B. C. Millar, and J. E. Moore, “Antimicrobial Resistance (AMR),” British Journal of Biomedical Science 80 (2023): 11387.

[45] K. A. Cohen, A. L. Manson, C. A. Desjardins, T. Abeel, and A. M. Earl, “Deciphering Drug Resistance in Mycobacterium Tuberculosis Using Whole-genome Sequencing: Progress, Promise, and Challenges,” Genome Medicine 11 (2019): 45.

[46] S. Wang, J. Yao, B. Zhou, et al., “Bacteriostatic Effect of Quercetin as an Antibiotic Alternative in Vivo and Its Antibacterial Mechanism in Vitro,” Journal of Food Protection 81, no. 1 (2018): 68–78.

[47] R. Hakenbeck, “Mosaic Genes and Their Role in Penicillin-resistant Streptococcus pneumoniae (minireview),” Electrophoresis 19 (1998): 597–601.

[48] C. R. Scharn, F. C. Tenover, and R. V. Goering, “Transduction of Staphylococcal Cassette ChromosomemecElements between Strains of Staphylococcus aureus,” Antimicrobial Agents and Chemotherapy 57 (2013): 5233–5238.

[49] K. A. Moser, L. Zhang, I. Spicknall, et al., “The Role of Mobile Genetic Elements in the Spread of Antimicrobial-Resistant Escherichia coli From Chickens to Humans in Small-Scale Production Poultry Operations in Rural Ecuador,” American Journal of Epidemiology 187 (2017): 558–567.

[50] F. Prestinaci, P. Pezzotti, and A. Pantosti, “Antimicrobial Resistance: A Global Multifaceted Phenomenon,” Pathogens and Global Health 109 (2015): 309–318.

[51] N. Dosoky and W. Setzer, “Chemical Composition and Biological Activities of Essential Oils of Curcuma Species,” Nutrients 10, no. 9 (2018): 1196.

[52] S. G. Santhosha, P. Jamuna, and S. N. Prabhavathi, “Bioactive Components of Garlic and Their Physiological Role in Health Maintenance: A Review,” Food Bioscience 3 (2013): 59–74.

[53] H. H. Phu, K. Pham Van, T. H. Tran, and D. T. N. Pham, “Extraction, Chemical Compositions and Biological Activities of Essential Oils of Cinnamomum Verum Cultivated in Vietnam,” Processes 10 (2022): 1713.

[54] A. Hazim, D. N. Rizkiyah, L. Qomariyah, I. Irianto, and N. R. Mohd Putra, “Unlocking the Full Potential of Clove (Syzygium aromaticum) Spice: An Overview of Extraction Techniques, Bioactivity, and Future Opportunities in the Food and Beverage Industry,” Processes 11 (2023): 2453–2453.

[55] W. Hu, A. Yu, S. Wang, et al., “Extraction, Purification, Structural Characteristics, Biological Activities, and Applications of the Polysaccharides from Zingiber Officinale Roscoe. (Ginger): A Review,” Molecules (Basel, Switzerland) 28 (2023): 3855.

[56] A. Milenković and L. Stanojević, “Black Pepper: Chemical Composition and Biological Activities,” Advanced Technologies 10 (2021): 40–50.

[57] O. Merah, B. Sayed-Ahmad, T. Talou, et al., “Biochemical Composition of Cumin Seeds, and Biorefining Study,” Biomolecules 10 (2020): 1054.

[58] Abdullah, A. Asghar, M. S. Butt, M. Shahid, and Q. Huang, “Evaluating the Antimicrobial Potential of Green Cardamom Essential Oil Focusing on Quorum Sensing Inhibition of Chromobacterium Violaceum,” Journal of Food Science and Technology 54, no. 8 (2017): 2306–2315.

[59] L. Hartanti, S. M. K. Yonas, J. J. Mustamu, S. Wijaya, H. K. Setiawan, and L. Soegianto, “Influence of Extraction Methods of Bay Leaves (Syzygium polyanthum) on Antioxidant and HMG-CoA Reductase Inhibitory Activity,” Heliyon 5 (2019): e01485.

[60] K. Ashokkumar, J. Simal-Gandara, M. Murugan, M. K. Dhanya, and A. Pandian, “Nutmeg (Myristica fragrans Houtt.) Essential Oil: A Review on Its Composition, Biological, and Pharmacological Activities,” Phytotherapy Research 36 (2022): 2839–2851.

[61] M. Ferreira, D. H. Kringel, D. de, R. Zavareze, and A. Renato, “Basil Essential Oil: Methods of Extraction, Chemical Composition, Biological Activities, and Food Applications,” Food and Bioprocess Technology 15 (2021): 1–27.

[62] J. Lietzow, “Biologically Active Compounds in Mustard Seeds: A Toxicological Perspective,” Foods 10 (2021): 2089.

[63] S. Akbari, N. H. Abdurahman, R. M. Yunus, O. R. Alara, and O. O. Abayomi, “Extraction, Characterization and Antioxidant Activity of Fenugreek (Trigonella-Foenum Graecum) Seed Oil,” Materials Science for Energy Technologies 2 (2019): 349–355.

[64] B. Ashaq, K. Rasool, S. Habib, et al., “Insights into Chemistry, Extraction and Industrial Application of Lemon Grass Essential Oil -A Review of Recent Advances,” Food Chemistry: X 22 (2024): 101521.

[65] S. Soltani, A. Shakeri, M. Iranshahi, and M. Boozari, “A Review of the Phytochemistry and Antimicrobial Properties of Origanum vulgare L. and Subspecies,” Iranian Journal of Pharmaceutical Research IJPR 20 (2021): 268–285.

[66] A. Amalraj, A. Pius, S. Gopi, and S. Gopi, “Biological Activities of Curcuminoids, Other Biomolecules from Turmeric and Their Derivatives—A Review,” Journal of Traditional and Complementary Medicine 7 (2017): 205–233.

[67] A. Shang, S. Cao, X. Xu, et al., “Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.),” Foods 8 (2019): 246.

[68] D. Kurnia, D. Ajiati, L. Heliawati, and D. Sumiarsa, “Antioxidant Properties and Structure-Antioxidant Activity Relationship of Allium Species Leaves,” Molecules (Basel, Switzerland) 26 (2021): 7175.

[69] P. Songvut, W. Nakareangrit, W. Cholpraipimolrat, et al., “Unraveling the Interconversion Pharmacokinetics and Oral Bioavailability of the Major Ginger Constituents: [6]-gingerol, [6]-shogaol, and Zingerone after Single-dose Administration in Rats,” Frontiers in Pharmacology 15 (2024): 1391019.

[70] S. Banerjee, P. Katiyar, V. Kumar, et al., “Black Pepper and Piperine Induce Anticancer Effects on Leukemia Cell Line,” Toxicology Research 10 (2021): 169–182.

[71] F. S. Mohammed, M. Sevindik, İ. Uysal, C. Çesko, and H. Koraqi, “Chemical Composition, Biological Activities, Uses, Nutritional and Mineral Contents of Cumin (Cuminum cyminum),” Measurement: Food 14 (2024): 100157.

[72] N. Abdullah, N. Ahmad, T. Wenni, et al., “Recent Advances in the Extraction, Chemical Composition, Therapeutic Potential, and Delivery of Cardamom Phytochemicals,” Frontiers in Nutrition 9 (2022): 1024820.

[73] S. Batool, R. A. Khera, M. A. Hanif, and M. A. Ayub, “Bay Leaf,” Medicinal Plants of South Asia (2020): 63–74, https://doi.org/10.1016/B978-0-08-102659-5.00005-7.

[74] M. E. Götz, B. Sachse, B. Schäfer, and A. Eisenreich, “Myristicin and Elemicin: Potentially Toxic Alkenylbenzenes in Food,” Foods 11 (2022): 1988.

[75] R. Joshi, “Chemical Composition and Antimicrobial Activity of the Essential Oil of Ocimum Basilicum L. (sweet basil) From Western Ghats of North West Karnataka, India,” Ancient Science of Life 33 (2014): 149.

[76] J. Gerendás, J. Podestát, T. Stahl, et al., “Interactive Effects of Sulfur and Nitrogen Supply on the Concentration of Sinigrin and Allyl Isothiocyanate in Indian Mustard (Brassica juncea L.),” Journal of Agricultural and Food Chemistry 57 (2009): 3837–3844.

[77] T. Visuvanathan, L. T. L. Than, J. Stanslas, S. Y. Chew, and S. Vellasamy, “Revisiting Trigonella Foenum-graecum L.: Pharmacology and Therapeutic Potentialities,” Plants 11 (2022): 1450.

[78] A. A. A. Murtadlo, A. N. M. Ansori, V. D. Kharisma, et al., “A Mini Review of Curcuma Longa: Antimicrobial Properties,” Journal of Medicinal and Chemical Sciences 7, no. 1 (2023): 215–221.

[79] K. Priyadarsini, “The Chemistry of Curcumin: From Extraction to Therapeutic Agent,” Molecules (Basel, Switzerland) 19, no. 12 (2014): 20091–20112.

[80] C. Dai, J. Lin, H. Li, et al., “The Natural Product Curcumin as an Antibacterial Agent: Current Achievements and Problems,” Antioxidants 11, no. 3 (2022): 459.

[81] A. Hassaninasab, Y. Hashimoto, K. Tomita-Yokotani, and M. Kobayashi, “Discovery of the Curcumin Metabolic Pathway Involving a Unique Enzyme in an Intestinal Microorganism,” Pnas 108, no. 16 (2011): 6615–6620.

[82] M. R. Riaz, S. A. Rauf, R. Lupoli, and A. R. Siddiqi, Potential of Turmeric Extract and Its Fractions to Control Peach Fruit Fly (Diptera: Tephritidae), Accessed January 1, 2024 (ResearchGate, 2015), https://www.researchgate.net/publication/285899967_Potential_of_turmeric_extract_and_its_fractions_to_control_peach_fruit_fly_Diptera_Tephritidae.

[83] E. Essien, J. Newby, T. Walker, W. Setzer, and O. Ekundayo, “Chemotaxonomic Characterization and in-Vitro Antimicrobial and Cytotoxic Activities of the Leaf Essential Oil of Curcuma Longa Grown in Southern Nigeria,” Medicines 2, no. 4 (2015): 340–349.

[84] A. Nair, A. Amalraj, J. Jacob, A. B. Kunnumakkara, and S. Gopi, “Non-Curcuminoids from Turmeric and Their Potential in Cancer Therapy and Anticancer Drug Delivery Formulations,” Biomolecules 9, no. 1 (2019): 13.

[85] Y. Di, A. Cao, Y. Zhang, and L. Zhang, Effects of Dietary 1,8-Cineole Supplementation on Growth Performance, Antioxidant Capacity, Immunity, and… ResearchGate, Accessed January 1, 2024 (2022), https://www.researchgate.net/publication/363578938_Effects_of_Dietary_18-Cineole_Supplementation_on_Growth_Performance_Antioxidant_Capacity_Immunity_and_Intestine_Health_of_Broilers.

[86] N. Arizmendi, S. B. Alam, K. Azyat, D. Makeiff, A. D. Befus, and M. Kulka, “The Complexity of Sesquiterpene Chemistry Dictates Its Pleiotropic Biologic Effects on Inflammation,” Molecules (Basel, Switzerland) 27, no. 8 (2022): 2450.

[87] S. Ankri and D. Mirelman, “Antimicrobial Properties of Allicin from Garlic,” Microbes and Infection 1, no. 2 (1999): 125–129.

[88] M. Nakamoto, K. Kunimura, J. Suzuki, and Y. Kodera, “Antimicrobial Properties of Hydrophobic Compounds in Garlic: Allicin, Vinyldithiin, Ajoene and Diallyl Polysulfides (Review),” Experimental and Therapeutic Medicine 19, no. 2 (2019): 1550–1553.

[89] R. Leontiev, N. Hohaus, C. Jacob, M. C. H. Gruhlke, and A. J. Slusarenko, “A Comparison of the Antibacterial and Antifungal Activities of Thiosulfinate Analogues of Allicin,” Scientific Reports 8, no. 1 (2018): 6763.

[90] Y. Tang, F. Li, D. Gu, W. Wang, J. Huang, and X. Jiao, “Antimicrobial Effect and the Mechanism of Diallyl Trisulfide against Campylobacter jejuni,” Antibiotics Basel 10, no. 3 (2021): 246–246.

[91] T. H. Jakobsen, A. N. Warming, R. M. Vejborg, et al., “A Broad Range Quorum Sensing Inhibitor Working through sRNA Inhibition,” Scientific Reports 7 (2017): 9857.

[92] R. Naganawa, N. Iwata, K. Ishikawa, H. Fukuda, T. Fujino, and A. Suzuki, “Inhibition of Microbial Growth by Ajoene, a Sulfur-containing Compound Derived from Garlic,” Applied and Environmental Microbiology 62, no. 11 (1996): 4238–4242.

[93] N. Rais, A. Ved, R. Ahmad, et al., “S-Allyl-L-Cysteine — A Garlic Bioactive: Physicochemical Nature, Mechanism, Pharmacokinetics, and Health Promoting Activities,” Journal of Functional Foods 107 (2023): 105657.

[94] K. Yukihiro, H. Matsuura, S. Yoshida, et al., “Allixin, a Stress Compound from Garlic,” Chemical & Pharmaceutical Bulletin 37, no. 6 (1989): 1656–1658.

[95] A. P. Li, Y. H. He, S. Y. Zhang, and Y. P. Shi, “Antibacterial Activity and Action Mechanism of Flavonoids against Phytopathogenic Bacteria,” Pesticide Biochemistry and Physiology 188 (2022): 105221.

[96] M. Silva, V. Caro, C. Guzmán, et al., “Chapter 1 - α-Synuclein and Tau, Two Targets for Dementia,” Studies in Natural Products Chemistry 67 (2020): 1–25, https://www.sciencedirect.com/science/article/abs/pii/B9780128194836000011.

[97] F. Usai and A. Di Sotto, “trans-Cinnamaldehyde as a Novel Candidate to Overcome Bacterial Resistance: An Overview of in Vitro Studies,” Antibiotics 12, no. 2 (2023): 254.

[98] F. Mousavi, B. Bojko, V. Bessonneau, and J. Pawliszyn, “Cinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography-Mass Spectrometry,” Journal of Proteome Research 15, no. 3 (2016): 963–975.

[99] Q. OuYang, X. Duan, L. Li, and N. Tao, “Cinnamaldehyde Exerts Its Antifungal Activity by Disrupting the Cell Wall Integrity of Geotrichum Citri-aurantii,” Frontiers in Microbiology 10 (2019): 55.

[100] S. H. Topa, E. A. Palombo, P. Kingshott, and L. L. Blackall, “Activity of Cinnamaldehyde on Quorum Sensing and Biofilm Susceptibility to Antibiotics in Pseudomonas aeruginosa,” Microorganisms 8, no. 3 (2020): 455.

[101] M. Ulanowska and B. Olas, “Biological Properties and Prospects for the Application of Eugenol—A Review,” International Journal of Molecular Sciences 22, no. 7 (2021): 3671.

[102] A. Marchese, R. Barbieri, E. Coppo, et al., “Antimicrobial Activity of Eugenol and Essential Oils Containing Eugenol: A Mechanistic Viewpoint,” Critical Reviews in Microbiology 43, no. 6 (2017): 668–689.

[103] M. K. M. Elbestawy, G. M. El-Sherbiny, and S. A. Moghannem, “Antibacterial, Antibiofilm and Anti-Inflammatory Activities of Eugenol Clove Essential Oil against Resistant Helicobacter pylori,” Molecules (Basel, Switzerland) 28, no. 6 (2023): 2448.

[104] M. Mingoia, C. Conte, A. Di Rienzo, et al., “Synthesis and Biological Evaluation of Novel Cinnamic Acid-Based Antimicrobials,” Pharmaceuticals (Basel) 15, no. 2 (2022): 228–228.

[105] H. Yang, X. Tuo, L. Wang, et al., “Bioactive Procyanidins from Dietary Sources: The Relationship between Bioactivity and Polymerization Degree,” Trends in Food Science & Technology 111 (2021): 114–127.

[106] M. M. G. Mattos, S. A. Filho, G. R. Martins, et al., “Antimicrobial and Antibiofilm Properties of Procyanidins: Potential for Clinical and Biotechnological Applications,” Critical Reviews in Microbiology (2024): 1–24, https://doi.org/10.1080/1040841x.2024.2404509.

[107] R. M. Alkufeidy, L. Ameer Altuwijri, N. S. Aldosari, N. Alsakabi, and T. M. Dawoud, “Antimicrobial and Synergistic Properties of Green Tea Catechins against Microbial Pathogens,” Journal of King Saud University - Science 36, no. 8 (2024): 103277–103277.

[108] S. S. Dahham, Y. M. Tabana, M. A. Iqbal, et al., “The Anticancer, Antioxidant and Antimicrobial Properties of the Sesquiterpene β-Caryophyllene from the Essential Oil of Aquilaria Crassna,” Molecules (Basel, Switzerland) 20, no. 7 (2015): 11808–11829.

[109] A. Scalbert, “Antimicrobial Properties of Tannins,” Phytochemistry 30, no. 12 (1991): 3875–3883.

[110] M. A. Elfaky, H. M. Okairy, H. M. Abdallah, et al., “Assessing the Antibacterial Potential of 6-gingerol: Combined Experimental and Computational Approaches,” Saudi Pharmaceutical Journal 32, no. 5 (2024): 102041.

[111] S. Y. Ham, H. S. Kim, M. J. Jo, et al., “Combined Treatment of 6-Gingerol Analog and Tobramycin for Inhibiting Pseudomonas aeruginosa Infections. Oglesby AG, Ed,” Microbiology Spectrum 9, no. 2 (2021): e0019221.

[112] A. H. Rahmani, F. M. A. Shabrmi, and S. M. Aly, “Active Ingredients of Ginger as Potential Candidates in the Prevention and Treatment of Diseases via Modulation of Biological Activities,” International Journal of Physiology, Pathophysiology and Pharmacology 6, no. 2 (2014): 125–136.

[113] T. Shelke, S. Talegaonkar, and M. Mishra, “Phytonanoparticles toward the Treatment of Diabetes,” Drug Delivery Systems for Metabolic Disorders (2022): 433–458, https://doi.org/10.1016/b978-0-323-99616-7.00027-x.

[114] L. Zou, Y. Y. Hu, and W. X. Chen, “Antibacterial Mechanism and Activities of Black Pepper Chloroform Extract,” Journal of Food Science and Technology 52, no. 12 (2015): 8196–8203.

[115] C. P. Bravo-Chaucanés, L. C. Chitiva, and Y. Vargas-Casanova, “Exploring the Potential Mechanism of Action of Piperine against Candida albicans and Targeting Its Virulence Factors,” Biomolecules 13, no. 12 (2023): 1729–1729.

[116] S. Arora, B. Singh, S. Kumar, A. Kumar, A. Singh, and C. Singh, “Piperine Loaded Drug Delivery Systems for Improved Biomedical Applications: Current Status and Future Directions,” Health Sciences Review 9 (2023): 100138.

[117] L. T. M. Hien and D. T. A. Dao, “Antibacterial Activity of Black Pepper Essential Oil Nanoemulsion Formulated by Emulsion Phase Inversion Method,” Current Research in Nutrition and Food Science Journal 10, no. 1 (2022): 311–320.

[118] P. V. Karsha and O. B. Lakshmi, “Antibacterial Activity of Black Pepper (Piper nigrum Linn.) With Special Reference to Its Mode of Action…,” Indian Journal of Natural Products and Resources 1 (2010): 213–215.

[119] N. Wongkattiya, P. Sanguansermsri, I. H. Fraser, and D. Sanguansermsri, “Antibacterial Activity of Cuminaldehyde on Food-borne Pathogens, the Bioactive Component of Essential Oil from Cuminum Cyminum L. collected in Thailand,” Journal of Complementary and Integrative Medicine 16, no. 4 (2019), https://doi.org/10.1515/jcim-2018-0195.

[120] V. Monteiro-Neto, C. D. de Souza, L. F. Gonzaga, et al., “Cuminaldehyde Potentiates the Antimicrobial Actions of Ciprofloxacin against Staphylococcus aureus and Escherichia coli. Omri A, Ed,” PLoS ONE 15, no. 5 (2020): e0232987.

[121] S. Bourgou, A. Pichette, B. Marzouk, and J. Legault, “Bioactivities of Black Cumin Essential Oil and Its Main Terpenes from Tunisia,” South African Journal of Botany 76, no. 2 (2010): 210–216.

[122] A. Gueffai, D. J. Gonzalez-Serrano, M. C. Christodoulou, et al., “Phenolics from Defatted Black Cumin Seeds (Nigella sativa L.): Ultrasound-Assisted Extraction Optimization, Comparison, and Antioxidant Activity,” Biomolecules 12, no. 9 (2022): 1311.

[123] C. C. Hoch, J. Petry, L. Griesbaum, et al., “1,8-cineole (eucalyptol): A Versatile Phytochemical with Therapeutic Applications across Multiple Diseases,” Biomedicine & Pharmacotherapy 167 (2023): 115467.

[124] L. Li, C. Shi, Z. Yin, et al., “Antibacterial Activity of α-terpineol May Induce Morphostructural Alterations in Escherichia coli,” Brazilian Journal of Microbiology 45, no. 4 (2015): 1409–1413.

[125] X. Yang, S. Zhao, Y. Deng, et al., “Antibacterial Activity and Mechanisms of α-terpineol against Foodborne Pathogenic Bacteria,” Applied Microbiology and Biotechnology 107, no. 21 (2023): 6641–6653.

[126] I. Noui Mehidi, A. Ait Ouazzou, W. Tachoua, and K. Hosni, “Investigating the Antimicrobial Properties of Essential Oil Constituents and Their Mode of Action,” Molecules (Basel, Switzerland) 29, no. 17 (2024): 4119–4119.

[127] V. Vaičiulytė, K. Ložienė, J. Švedienė, V. Raudonienė, and A. Paškevičius, “α-Terpinyl Acetate: Occurrence in Essential Oils Bearing Thymus Pulegioides, Phytotoxicity, and Antimicrobial Effects,” Molecules (Basel, Switzerland) 26, no. 4 (2021): 1065.

[128] S. Zhou, C. Wei, C. Zhang, C. Han, N. Kuchkarova, and H. Shao, “Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum Integrifolium,” Toxins 11, no. 10 (2019): 598.

[129] I. D. Dewijanti, W. Mangunwardoyo, and A. Dwianti, “Antimicrobial Activity of Bay Leaf (Syzygium polyanthum (wight) Walp) Extracted Using Various Solvent,” in Proc 5TH Int Symp Appl Chem 2019, https://doi.org/10.1063/1.5134585, Published online 2019.

[130] Z. Shafiei, N. N. Shuhairi, N. Md Fazly Shah Yap, C. A. Harry Sibungkil, and J. Latip, “Antibacterial Activity of Myristica Fragrans against Oral Pathogens,” Evidence-Based Complementary and Alternative Medicine ECAM 2012 (2012): 825362.

[131] E. F. Seneme, D. C. dos Santos, E. M. R. Silva, Y. E. M. Franco, and G. B. Longato, “Pharmacological and Therapeutic Potential of Myristicin: A Literature Review,” Molecules (Basel, Switzerland) 26, no. 19 (2021): 5914.

[132] Q. P. S. Barbosa, C. A. G. da Câmara, C. S. Ramos, D. C. O. Nascimento, J. V. Lima-Filho, and E. F. Guimarães, “Chemical Composition, Circadian Rhythm and Antibacterial Activity of Essential Oils of Piper Divaricatum: A New Source of Safrole,” Quím Nova 35 (2012): 1806–1808.

[133] M. Moghaddam, M. R. Alymanesh, L. Mehdizadeh, H. Mirzaei, and A. Ghasemi Pirbalouti, “Chemical Composition and Antibacterial Activity of Essential Oil of Ocimum Ciliatum, as a New Source of Methyl Chavicol, against Ten Phytopathogens,” Industrial Crops and Products 59 (2014): 144–148.

[134] A. Abdollahi, N. Fereydouni, H. Moradi, A. Karimivaselabadi, E. Zarenezhad, and M. Osanloo, “Nanoformulated Herbal Compounds: Enhanced Antibacterial Efficacy of Camphor and Thymol-loaded Nanogels,” BMC Complementary Medicine and Therapies 24, no. 1 (2024): 138.

[135] C. Zimmermann, S. Dähn, and A. E. Wagner, “Effect of Allyl-isothiocyanate on Survival and Antimicrobial Peptide Expression Following Oral Bacterial Infections in Drosophila Melanogaster,” Frontiers in Immunology 15 (2024): 1404086.

[136] A. Abreu, A. Borges, L. Simoes, M. Saavedra, and M. Simoes, “Antibacterial Activity of Phenyl Isothiocyanate on Escherichia coli and Staphylococcus aureus,” Medicinal Chemistry 9, no. 5 (2013): 756–761.

[137] A. Galanty, M. Grudzińska, W. Paździora, and P. Paśko, “Erucic Acid—Both Sides of the Story: A Concise Review on Its Beneficial and Toxic Properties,” Molecules (Basel, Switzerland) 28, no. 4 (2023): 1924.

[138] A. Raquel, J. Santos, M. Barbosa, et al., “Evaluation of the Antibacterial and Inhibitory Activity of NorA and MepA Efflux Pumps from Staphylococcus aureus by Diosgenin,” Life Sciences 308 (2022): 120978–120978.

[139] A. Kar, S. Kumar Mukherjee, S. Barik, and S. Tofajjen Hossain, “Antimicrobial Activity of Trigonelline Hydrochloride Against Pseudomonas aeruginosa and Its Quorum-Sensing Regulated Molecular Mechanisms on Biofilm Formation and Virulence,” ACS Infectious Diseases 10, no. 2 (2024): 746–762.

[140] S. Yadav, A. K. Singh, A. K. Agrahari, et al., “Making of Water Soluble Curcumin to Potentiate Conventional Antimicrobials by Inducing Apoptosis-Like Phenomena among Drug-resistant Bacteria,” Scientific Reports 10 (2020): 14204.

[141] L. Zhong, Y. Lin, C. Wang, et al., “Chemical Profile, Antimicrobial and Antioxidant Activity Assessment of the Crude Extract and Its Main Flavonoids From Tartary Buckwheat Sprouts,” Molecules (Basel, Switzerland) 27, no. 2 (2022): 374.

[142] A. Fajdek-Bieda, J. Pawlińska, A. Wróblewska, and A. Łuś, “Evaluation of the Antimicrobial Activity of Geraniol and Selected Geraniol Transformation Products against Gram-Positive Bacteria,” Molecules (Basel, Switzerland) 29, no. 5 (2024): 950.

[143] M. I. Naik, B. A. Fomda, E. Jaykumar, and J. A. Bhat, “Antibacterial Activity of Lemongrass (Cymbopogon citratus) Oil against some Selected Pathogenic Bacterias,” Asian Pacific Journal of Tropical Medicine 3 (2010): 535–538.

[144] J. R. Scherf, C. R. Barbosa dos Santos, T. Sampaio de Freitas, et al., “Effect of Terpinolene against the Resistant Staphylococcus aureus Strain, Carrier of the Efflux Pump QacC and β-lactamase Gene, and Its Toxicity in the Drosophila Melanogaster Model,” Microbial Pathogenesis 149 (2020): 104528.

[145] L. Dedieu, J. M. Brunel, V. Lorenzi, A. Muselli, L. Berti, and J. M. Bolla, “Antibacterial Mode of Action of the Daucus Carota Essential Oil Active Compounds against Campylobacter Jejuni and Efflux-Mediated Drug Resistance in Gram-Negative Bacteria,” Molecules (Basel, Switzerland) 25, no. 22 (2020): 5448.

[146] L. Tao, Y. Liang, Z. Xia, et al., “Antibacterial Activities of Oregano Essential Oils and Their Active Components,” Frontiers in Pharmacology 16 (2025): 1579283.

[147] A. Hajibonabi, M. Yekani, S. Sharifi, J. S. Nahad, S. M. Dizaj, and M. Y. Memar, “Antimicrobial Activity of Nanoformulations of Carvacrol and Thymol: New Trend and Applications,” OpenNano 13 (2023): 100170.

[148] M. Aydin, N. Unusan, E. Sumlu, and E. N. Korucu, “Rosmarinic Acid Exhibits Antifungal and Antibiofilm Activities Against Candida albicans: Insights into Gene Expression and Morphological Changes,” Journal of Fungi 10 (2024): 751.

[149] N. Ghadimi, L. Asadpour, and M. Mokhtary, “Enhanced Antimicrobial, Anti-biofilm, and Efflux Pump Inhibitory Effects of Ursolic Acid-conjugated Magnetic Nanoparticles against Clinical Isolates of Multidrug-resistant Pseudomonas aeruginosa,” Microbial Pathogenesis 199 (2025): 107241.

[150] S. Nabavi, A. Di Lorenzo, M. Izadi, E. Sobarzo-Sánchez, M. Daglia, and S. Nabavi, “Antibacterial Effects of Cinnamon: From Farm to Food, Cosmetic and Pharmaceutical Industries,” Nutrients 7, no. 9 (2015): 7729–7748.

[151] Y. Hussain, W. Alam, H. Ullah, et al., “Antimicrobial Potential of Curcumin: Therapeutic Potential and Challenges to Clinical Applications,” Antibiotics 11, no. 3 (2022): 322.

[152] Z. Al Noman, T. Tabassum Anika, S. Sachi, et al., “Evaluation of Antibacterial Efficacy of Garlic (Allium sativum) and Ginger (Zingiber officinale) Crude Extract against Multidrug-resistant (MDR) Poultry Pathogen,” Journal of Advanced Veterinary and Animal Research 10, no. 2 (2023): 151–151.

[153] J. Bai, J. Li, Z. Chen, et al., “Antibacterial Activity and Mechanism of Clove Essential Oil against Foodborne Pathogens,” LWT 173 (2023): 114249.

[154] X. Wang, Y. Shen, K. Thakur, et al., “Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus,” Molecules (Basel, Switzerland) 25, no. 17 (2020): 3955.

[155] J. Zhang, K. P. Ye, X. Zhang, D. D. Pan, Y. Y. Sun, and J. X. Cao, “Antibacterial Activity and Mechanism of Action of Black Pepper Essential Oil on Meat-Borne Escherichia coli,” Frontiers in Microbiology 7 (2017): 2094.

[156] E. A. Rodrigues dos Santos, L. Ereno Tadielo, J. Arruda Schmiedt, et al., “Inhibitory Effects of Piperine and Black Pepper Essential Oil on Multispecies Biofilm Formation by Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa,” LWT 182 (2023): 114851.

[157] A. Abbaszadegan, A. Gholami, Y. Ghahramani, et al., “Antimicrobial and Cytotoxic Activity of Cuminum Cyminum as an Intracanal Medicament Compared to Chlorhexidine Gel,” Iranian Endodontic Journal 11, no. 1 (2016): 44–50.

[158] J. Wanner, S. Bail, L. Jirovetz, et al., “Chemical Composition and Antimicrobial Activity of Cumin Oil (Cuminum Cyminum, Apiaceae),” Natural Products Communications 5, no. 9 (2010): 1934578×1000500.

[159] S. M. da Silveira, F. B. Luciano, N. Fronza, A. Cunha, G. N. Scheuermann, and C. R. W. Vieira, “Chemical Composition and Antibacterial Activity of Laurus Nobilis Essential Oil towards Foodborne Pathogens and Its Application in Fresh Tuscan Sausage Stored at 7°C,” LWT - Food Science and Technology 59, no. 1 (2014): 86–93.

[160] K. A. Panggabean, H. Rusmarilin, and D. Suryanto, “The Utilization of Nutmeg Seed (Myristica fragrans Houtt) Extract as an Antimicrobial on Tempeh Sausage,” IOP Conference Series: Earth and Environmental Science 260 (2019): 012087.

[161] C. A. Semeniuc, C. R. Pop, and A. M. Rotar, “Antibacterial Activity and Interactions of Plant Essential Oil Combinations against Gram-positive and Gram-negative Bacteria,” Journal of Food and Drug Analysis 25, no. 2 (2017): 403–408.

[162] B. Schweitzer, V. L. Balázs, S. Molnár, et al., “Antibacterial Effect of Lemongrass (Cymbopogon citratus) against the Aetiological Agents of Pitted Keratolyis,” Molecules (Basel, Switzerland) 27, no. 4 (2022): 1423.

[163] M. Walasek-Janusz, A. Grzegorczyk, A. Malm, R. Nurzyńska-Wierdak, and D. Zalewski, “Chemical Composition, and Antioxidant and Antimicrobial Activity of Oregano Essential Oil,” Molecules (Basel, Switzerland) 29 (2024): 435–435.

[164] D. Gwiazdowska, A. Waśkiewicz, K. Juś, et al., “Antimicrobial and Antibiofilm Activity of Origanum vulgare Extracts Obtained by Supercritical Fluid Extraction Under Various Extraction Conditions,” Molecules (Basel, Switzerland) 29 (2024): 5823.

[165] J. W. He, M. Hadidi, S. Yang, M. R. Khan, W. Zhang, and X. Cong, “Natural Food Preservation with Ginger Essential Oil: Biological Properties and Delivery Systems,” Food Research International 173 (2023): 113221–113221.

[166] S. Chatterjee, P. Paul, P. Chakraborty, et al., “Cuminaldehyde Exhibits Potential Antibiofilm Activity against Pseudomonas aeruginosa Involving Reactive Oxygen Species (ROS) Accumulation: A Way Forward towards Sustainable Biofilm Management,” 3 Biotech 11 (2021): 485.

[167] R. Irkin and O. K. Esmer, “Novel Food Packaging Systems with Natural Antimicrobial Agents,” Journal of Food Science and Technology 52 (2015): 6095–6111.

[168] J. Ahmed, E. Altun, M. O. Aydogdu, et al., “Anti-fungal Bandages Containing Cinnamon Extract,” International Wound Journal 16, no. 3 (2019): 730–736.

[169] Y. Dai, M. Gultekinoglu, C. Bayram, H. B. De Silva, and M. Edirisinghe, “Antibacterial Properties of Natural Cinnamon-alginate Fibrous Patches Produced by Modified Nozzle-pressurized Spinning,” MedComm 5, no. 9 (2024): e731.

[170] C. Kotlowski, P. Aspermair, H. Ullah Khan, et al., “Electronic Biosensing with Flexible Organic Transistor Devices,” Flexible and Printed Electronics 3, no. 3 (2018): 034003–034003.

[171] J. Wang, X. Zhou, W. Li, X. Deng, Y. Deng, and X. Niu, “Curcumin Protects Mice from Staphylococcus aureus Pneumonia by Interfering with the Self-assembly Process of α-hemolysin,” Scientific Reports 6 (2016): 28254.

[172] R. Wang, L. van Dorp, L. P. Shaw, et al., “The Global Distribution and Spread of the Mobilized Colistin Resistance Gene Mcr-1,” Nature Communications 9 (2018): 1179.

[173] G. Hellen, J. A. Santos, and M. Soares, “In Vitro and in Vivo Antibacterial Activity Assays of Carvacrol: A Candidate for Development of Innovative Treatments against KPC-producing Klebsiella pneumoniae,” PLoS ONE 16 (2021): e0246003.

[174] W. A. Pereira, C. D. S. Pereira, R. G. Assunção, et al., “New Insights into the Antimicrobial Action of Cinnamaldehyde towards Escherichia coli and Its Effects on Intestinal Colonization of Mice,” Biomolecules 11 (2021): 302.

[175] I. F. S. Figueiredo, L. G. Araújo, R. G. Assunção, et al., “Cinnamaldehyde Increases the Survival of Mice Submitted to Sepsis Induced by Extraintestinal Pathogenic Escherichia coli,” Antibiotics 11 (2022): 364.

[176] J. Li, T. Lu, Y. Chu, et al., “Cinnamaldehyde Targets SarA to Enhance β-lactam Antibiotic Activity against Methicillin-resistant Staphylococcus aureus,” mLife 3 (2024): 291–306.

[177] H. B. van der Worp, D. W. Howells, E. S. Sena, et al., “Can Animal Models of Disease Reliably Inform Human Studies?,” Plos Medicine 7 (2010): e1000245.

[178] C. Bitwell, S. S. Indra, C. Luke, and M. K. Kakoma, “A Review of Modern and Conventional Extraction Techniques and Their Applications for Extracting Phytochemicals from Plants,” Scientific African 19 (2023): e01585.

[179] A. S. Caballero Galván, C. E. O. Alzate, and C. Á. Cardona Álzate, Economic Assessment of Polyphenolic Compounds Production at Different Purities and Applications (2017), https://www.researchgate.net/publication/330369288_Economic_assessment_of_polyphenolic_compounds_production_at_different_purities_and_applications.

[180] A. E. Atabani, A. S. Silitonga, H. C. Ong, et al., “Non-edible Vegetable Oils: A Critical Evaluation of Oil Extraction, Fatty Acid Compositions, Biodiesel Production, Characteristics, Engine Performance and Emissions Production,” Renewable and Sustainable Energy Reviews 18 (2013): 211–245.

[181] P. Kapadia, A. S. Newell, J. Cunningham, M. R. Roberts, and J. G. Hardy, “Extraction of High-Value Chemicals from Plants for Technical and Medical Applications,” International Journal of Molecular Sciences 23, no. 18 (2022): 10334.

[182] Shahid-ul-Islam, M. Shahid, and F. Mohammad, “Perspectives for Natural Product Based Agents Derived from Industrial Plants in Textile Applications—A Review,” Journal of Cleaner Production 57 (2013): 2–18.

[183] P. Tongnuanchan and S. Benjakul, “Essential Oils: Extraction, Bioactivities, and Their Uses for Food Preservation,” Journal of Food Science 79, no. 7 (2014): R1231–R1249.

[184] A. Rafiq, B. Manzoor, M. A. Nayeem, A. Jabeen, and Q. A. Amin, Extraction of Essential Oils, Published online (Elsevier EBooks, 2024), 279–298, https://doi.org/10.1016/b978-0-12-819516-1.00005-3.

[185] A. Alves Prestes, M. Helena, C. Vieira Helm, and A. G. Cruz, “Elane Schwinden Prudencio. The Use of Cold Pressing Technique Associated with Emerging Nonthermal Technologies in the Preservation of Bioactive Compounds in Tropical Fruit Juices: An Overview,” Current Opinion in Food Science 51 (2023): 101005–101005.

[186] M. Gonzalez-Miquel and J. Esteban, Novel Solvents for Biotechnology Applications, Published online (2019), 790–806, https://doi.org/10.1016/b978-0-444-64046-8.00459-6.

[187] I. S. Pratama, Y. Putra, R. Pangestuti, S. K. Kim, and E. A. Siahaan, “Bioactive Peptides-derived from Marine by-products: Development, Health Benefits and Potential Application in Biomedicine,” Fisheries and Aquatic Sciences 25, no. 7 (2022): 357–379.

[188] C. J. Qin, “Spices and Flavoring (Flavouring) Crops | Properties and Analysis,” in Elsevier EBooks, Published online January (2003), 5491–5501, https://doi.org/10.1016/b0-12-227055-x/01126-3.

[189] V. I. Sousa, J. F. Parente, J. F. Marques, M. A. Forte, and C. J. Tavares, “Microencapsulation of Essential Oils: A Review,” Polymers 14, no. 9 (2022): 1730.

[190] H. M. A. AlSheikh, I. Sultan, V. Kumar, et al., “Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance,” Antibiotics 9, no. 8 (2020): 480.

[191] A. Shariati, M. Noei, M. Askarinia, A. Khoshbayan, A. Farahani, and Z. Chegini, “Inhibitory Effect of Natural Compounds on Quorum Sensing System in Pseudomonas aeruginosa: A Helpful Promise for Managing Biofilm Community,” Frontiers in Pharmacology 15 (2024): 1350391.

[192] A. Avakh, G. Grant, M. J. Cheesman, T. Kalkundri, and S. Hall, “The Art of War with Pseudomonas aeruginosa: Targeting Mex Efflux Pumps Directly to Strategically Enhance Antipseudomonal Drug Efficacy,” Antibiotics 12 (2023): 1304–1304.

[193] N. S. Sundaramoorthy, A. Sivasubramanian, and S. Nagarajan, “Simultaneous Inhibition of MarR by Salicylate and Efflux Pumps by Curcumin Sensitizes Colistin Resistant Clinical Isolates of Enterobacteriaceae,” Microbial Pathogenesis 148 (2020): 104445.

[194] M. Ghasemi, M. H. Nowroozzadeh, F. Ghorat, A. Iraji, and M. H. Hashempur, “Piperine and Its Nanoformulations: A Mechanistic Review of Their Anti-cancer Activities,” Biomedicine and Pharmacotherapy 187 (2025): 118075.

[195] N. R. Khanna and V. Gerriets, Beta Lactamase Inhibitors, Published online September (2022), https://www.ncbi.nlm.nih.gov/books/NBK557592/.

[196] K. S. fang, X. J, C. Y. tao, P. X. xian, H. Li, and B. Peng, “Exogenous Pyruvate Promotes Gentamicin Uptake to Kill Antibiotic-resistant Vibrio Alginolyticus,” International Journal of Antimicrobial Agents 63 (2024): 107036–107036.

[197] B. R. Wagle, A. Upadhyay, I. Upadhyaya, et al., “Trans-Cinnamaldehyde, Eugenol and Carvacrol Reduce Campylobacter Jejuni Biofilms and Modulate Expression of Select Genes and Proteins,” Frontiers in Microbiology 10 (2019): 1837.

[198] T. H. Jakobsen, M. van Gennip, R. K. Phipps, et al., “Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing,” Antimicrobial Agents and Chemotherapy 56 (2012): 2314–2325.

[199] K. Farhadi, E. Rajabi, H. A. Varpaei, M. Iranzadasl, S. Khodaparast, and M. Salehi, “Thymol and Carvacrol against Klebsiella: Anti-bacterial, Anti-biofilm, and Synergistic Activities—a Systematic Review,” Frontiers in Pharmacology 15 (2024): 1487083.

[200] R. Bernini and F. Velotti, “Natural Polyphenols as Immunomodulators to Rescue Immune Response Homeostasis: Quercetin as a Research Model against Severe COVID-19,” Molecules (Basel, Switzerland) 26 (2021): 5803.

[201] H. Dong, G. Song, Z. Wang, X. Wu, Q. Wang, and Y. H. Wang, “Kaempferol as a Multifaceted Immunomodulator: Implications for Inflammation, Autoimmunity, and Cancer,” Frontiers in Immunology 16 (2025): 1671519.

[202] R. E. Glover, A. C. Singer, A. P. Roberts, and C. Kirchhelle, “NIMble Innovation—a Networked Model for Public Antibiotic Trials,” The Lancet Microbe 2, no. 11 (2021): e637–e644.

[203] E. Zhang, “Turmeric-Induced Liver Injury in a Patient with Concurrent Semaglutide Use,” American Journal of Hospital Medicine 8 (2024), 10.24150/ajhm/2023.012.

[204] D. Soleimani, Z. Paknahad, and M. H. Rouhani, “Therapeutic Effects of Garlic on Hepatic Steatosis in Nonalcoholic Fatty Liver Disease Patients: A Randomized Clinical Trial,” Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 13 (2020): 2389–2397.

[205] N. Iwata, M. Kainuma, D. Kobayashi, et al., “The Relation between Hepatotoxicity and the Total Coumarin Intake from Traditional Japanese Medicines Containing Cinnamon Bark,” Frontiers in Pharmacology 7 (2016): 174.

[206] LiverTox Eugenol (Clove Oil), Published online 2012, https://www.ncbi.nlm.nih.gov/books/NBK551727/.

[207] M. Nikkhah Bodagh, I. Maleki, and A. Hekmatdoost, “Ginger in Gastrointestinal Disorders: A Systematic Review of Clinical Trials,” Food Science & Nutrition 7 (2019): 96–108.

[208] R. Ziegenhagen, K. Heimberg, A. Lampen, and K. I. Hirsch-Ernst, “Safety Aspects of the Use of Isolated Piperine Ingested as a Bolus,” Foods 10 (2021): 2121.

[209] H. Çelik and E. Kumaş, “Natural Aldehydes on Health Effects,” Oriental Journal of Chemistry 41 (2025), https://www.orientjchem.org/vol41no4/natural-aldehydes-on-health-effects/.

[210] V. Bampidis, G. Azimonti, M. de Lourdes Bastos, et al., “Safety and Efficacy of an Essential Oil from Elettaria Cardamomum (L.) Maton When Used as a Sensory Additive in Feed for all Animal Species,” EFSA Journal 17 (2019): e05721.

[211] H. J. Sipe, O. M. Lardinois, and R. P. Mason, “Free Radical Metabolism of Methyleugenol and Related Compounds,” Chemical Research in Toxicology 27 (2014): 483–489.

[212] K. Usui, E. Kubota, H. Kobayashi, et al., “Detection of Major Psychoactive Compounds (safrole, myristicin, and elemicin) of Nutmeg in human Serum via GC-MS/MS Using MonoSpin® Extraction: Application in a Nutmeg Poisoning Case,” Journal of Pharmaceutical and Biomedical Analysis 234 (2023): 115565.

[213] A. Eisenreich, L. Wittek, M. Sagmeister, et al., “Comparative Analysis of Estragole, Methyleugenol, Myristicin, and Elemicin Regarding Micronucleus Formation in V79 Cells,” Molecules (Basel, Switzerland) 30 (2025): 806–806.

[214] W. Alibrahem, H. Nguyen, N. K. Helu, et al., “Health Benefits, Applications, and Analytical Methods of Freshly Produced Allyl Isothiocyanate,” Foods 14 (2025): 579–579.

[215] M. Shabil, G. Bushi, P. K. Bodige, et al., “Effect of Fenugreek on Hyperglycemia: A Systematic Review and Meta-Analysis,” Medicina 59 (2023): 248.

[216] A. Sindle and K. Martin, “Essential Oils—Natural Products Not Necessarily Safe,” International Journal of Women's Dermatology 7 (2020): 304–308.

[217] M. Llana-Ruiz-Cabello, D. Gutiérrez-Praena, S. Pichardo, et al., “Cytotoxicity and Morphological Effects Induced by Carvacrol and Thymol on the human Cell Line Caco-2,” Food and Chemical Toxicology 64 (2014): 281–290.

[218] S. C. Gupta, S. Patchva, and B. B. Aggarwal, “Therapeutic Roles of Curcumin: Lessons Learned from Clinical Trials,” The Aaps Journal [Electronic Resource] 15, no. 1 (2012): 195–218.

[219] G. Hartnoll, D. Moore, and D. Douek, “Near Fatal Ingestion of Oil of Cloves,” Archives of Disease in Childhood 69, no. 3 (1993): 392–393.

[220] J. E. Ehrenpreis, C. DesLauriers, P. Lank, P. K. Armstrong, and J. B. Leikin, “Nutmeg Poisonings: A Retrospective Review of 10 Years Experience from the Illinois Poison Center, 2001–2011,” Journal of Medical Toxicology 10, no. 2 (2014): 148–151.

[221] T. Yamada, N. Katsutani, T. Maruyama, et al., “Combined Risk Assessment of Food-derived Coumarin with in Silico Approaches,” Food Safety 10, no. 3 (2022): 73–82.

[222] A. Vidalis, O. Dumoulin, M. Godbole, and C. C. Proenca, “The Role and Value of Real-world Evidence in Health Technology Decision-making in France, Germany, Italy, Spain, and the UK: Insights on External Control Arms,” International Journal of Technology Assessment in Health Care 41 (2025): e25.

[223] D. H. Ryu, J. Y. Cho, S. H. Yang, and H. Y. Kim, “Effects of Harvest Timing on Phytochemical Composition in Lamiaceae Plants under an Environment-Controlled System,” Antioxidants 12 (2023): 1909–1909.

[224] S. Govindaraghavan and N. J. Sucher, “Quality Assessment of Medicinal Herbs and Their Extracts: Criteria and Prerequisites for Consistent Safety and Efficacy of Herbal Medicines,” Epilepsy & Behavior 52 (2015): 363–371.

[225] R. Tabanelli, S. Brogi, and V. Calderone, “Improving Curcumin Bioavailability: Current Strategies and Future Perspectives,” Pharmaceutics 13 (2021): 1715.

[226] Z. Goktas, Y. Zu, M. Abbasi, et al., “Recent Advances in Nanoencapsulation of Phytochemicals to Combat Obesity and Its Comorbidities,” Journal of Agricultural and Food Chemistry 68 (2020): 8119–8131.

[227] M. Gera, N. Sharma, M. Ghosh, et al., “Nanoformulations of Curcumin: An Emerging Paradigm for Improved Remedial Application,” Oncotarget 8 (2017): 66680–66698.

[228] D. Yang, H. Eun, and C. Pricilia, “Metabolic Engineering and Synthetic Biology Approaches for the Heterologous Production of Aromatic Polyketides,” International Journal of Molecular Sciences 24 (2023): 8923–8923.

[229] A. Gangwal and A. Lavecchia, “Artificial Intelligence in Natural Product Drug Discovery: Current Applications and Future Perspectives,” Journal of Medicinal Chemistry 68, no. 4 (2025): 3948–3969, Published online February 2025.

[230] M. Ghazi Vakili, C. Gorgulla, J. Snider, et al., “Quantum-computing-enhanced Algorithm Unveils Potential KRAS Inhibitors,” Nature Biotechnology 43, no. 12 (2025): 1954–1959, Published online January 2025.

[231] S. Salm, J. Rutz, R. A. van Blaheta, and B. E. Bachmeier, “Current state of Research on the Clinical Benefits of Herbal Medicines for Non-life-threatening Ailments,” Frontiers in Pharmacology 14 (2023): 1234701.

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