N1-methylpseudouridine modification level correlates with protein expression, immunogenicity, and stability of mRNA

Shaoyi Chen , Zheng Liu , Jingsheng Cai , Haoran Li , Mantang Qiu

MedComm ›› 2024, Vol. 5 ›› Issue (10) : e691

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MedComm ›› 2024, Vol. 5 ›› Issue (10) : e691 DOI: 10.1002/mco2.691
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N1-methylpseudouridine modification level correlates with protein expression, immunogenicity, and stability of mRNA

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Shaoyi Chen, Zheng Liu, Jingsheng Cai, Haoran Li, Mantang Qiu. N1-methylpseudouridine modification level correlates with protein expression, immunogenicity, and stability of mRNA. MedComm, 2024, 5(10): e691 DOI:10.1002/mco2.691

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References

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Chen YG, Kim MV, Chen X, et al. Sensing self and foreign circular RNAs by intron identity. Mol Cell. 2017; 67(2): 228-238. e5.

[2]

Tanji H, Ohto U, Shibata T, et al. Toll-like receptor 8 senses degradation products of single-stranded RNA. Nat Struct Mol Biol. 2015; 22(2): 109-915.

[3]

Andries O, Mc Cafferty S, De Smedt SC, Weiss R, Sanders NN, Kitada T. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015; 217: 337-344.

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Mauger DM, Cabral BJ, Presnyak V, et al. mRNA structure regulates protein expression through changes in functional half-life. Proc Natl Acad Sci U S A. 2019; 116(48): 24075-24083.

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Svitkin YV, Gingras A-C, Sonenberg N. Membrane-dependent relief of translation elongation arrest on pseudouridine-and N1-methyl-pseudouridine-modified mRNAs. Nucleic Acids Res. 2021; 50(13): 7202-7215.

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2024 The Author(s). MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd.

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