Bovine Serum Albumin over Graphene Oxide Surface: Binding Capacity and Interaction

Abstract

We investigated the binding of universal protein BSA (Bovine Serum Albumin) over graphene oxide (GO) surface and performed quantitative measurements to find its maximum binding capacity. The interaction of proteins onto a 2D surface can happen via two available orthogonal extremes-side-on and end-on. Electrochemical measurements using cyclic voltammetry to define the uniqueness of the present study as it confirms the binding of BSA over GO surface and evaluate its maximum capacity. The reliability of the results is verified by repeating the experiment multiple times over a year. UV-Vis, FTIR, Raman, and SEM measurements clearly show that BSA is perfectly bound over GO. The estimated value of BSA over GO calculated by standard protein estimation test - Lowry’s protein assay is 1.1 mg/mg.

Keywords

Graphene oxide / Bovine Serum Albumin / Binding capacity / Lowry’s protein assay / Cyclic voltammetry

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