Construction of Magnetic Microbes for Oriented Self-healing of Mortar Cracks

Abstract

Sporosarcina pasteurii was employed as the strain, with an in-situ magnetization construction, to obtain magnetic microorganisms and oriented self-healing mortar specimens based on them. The magnetic field was used to achieve the directional migration of magnetic microorganisms during the oriented self-healing of mortar cracks, improving the rate of self-healing of cracks. The experimental results demonstrate that the magnetic microorganisms are composed of Fe₃O₄ nanosheets attached to the surface of Sporosarcina pasteurii, whose mineralization products are comprised of vaterite primarily. Compared with the pure microbial group, the magnetic microbial group exhibits a faster repair rate, shortening the repair time required to achieve an area repair efficiency of over 90% from 28 days to 14 days, thereby doubling the repair rate. Meanwhile, the area repair efficiency of the magnetic microbial group at 7, 14, and 28 days are increased by 50.3%, 11.2%, and 4.6%, respectively, compared to the pure microbial group, which are due to the magnetic microorganisms’ superior directional migration and mineralization ability, exceeding that of the ordinary microorganisms.

Keywords

Sporosarcina pasteurii / magnetic microorganisms / oriented self-healing mortar / self-healing rate / directional migration

References

[1] Jonkers HM, Thijssen A, Muyzer G, et al. . Application of Bacteria as Self-healing Agent for the Development of Sustainable Concrete. Ecological Engineering, 2010, 36(2): 230-235 J]

[2] Granger S, Loukili A, Pijaudier-Cabot G, et al. . Experimental Characterization of the Self-healing of Cracks in an Ultra High Performance Cementitious Material: Mechanical Tests and Acoustic Emission Analysis. Cement and Concrete Research, 2007, 37(4): 519-527 J]

[3] Xu H, Lian J, Gao M, et al. . Self-healing Concrete Using Rubber Particles to Immobilize Bacterial Spores. Materials, 2019, 12(14): 2 313 J]

[4] Ruan S, Qiu J, Weng Y, et al. . The Use of Microbial Induced Carbonate Precipitation in Healing Cracks within Reactive Magnesia Cement-Based Blends. Cement and Concrete Research, 2019, 115: 176-188 J]

[5] Zeng MH, Wu ZM, Wang YJ. A Stochastic Model Considering Heterogeneity and Crack Propagation in Concrete. Construction and Building Materials, 2020, 254: 119 289 J]

[6] Nain N, Surabhi R, Yathish NV, et al. . Enhancement in Strength Parameters of Concrete by Application of Bacillus Bacteria. Construction and Building Materials, 2019, 202: 904-908 J]

[7] Peng J, Hu S, Zhang J, et al. . Influence of Cracks on Chloride Diffusivity in Concrete: A Five-phase Mesoscale Model Approach. Construction and Building Materials, 2019, 197: 587-596 J]

[8] Lu WJ, Qian CX, Wang RX. Study on Soil Solidification Based on Microbiological Precipitation of CaCO₃. Science China (Technological Sciences), 2010, 53(09): 2 372-2 377 J]

[9] Kim TK, Park JS. Performance Evaluation of Concrete Structures Using Crack Repair Methods. Sustainability, 2021, 13(6): 3 217 J]

[10] Amran M, Onaizi AM, Fediuk R, et al. . Self-Healing Concrete as a Prospective Construction Material: A Review. Materials., 2022, 15(9): 3 214 J]

[11] Seifan M, Berenjian A. Application of Microbially Induced Calcium Carbonate Precipitation in Designing Bio Self-healing Concrete. World Journal of Microbiology and Biotechnology., 2018, 34(11): 168 J]

[12] Wiktor V, Jonkers HM. Quantification of Crack-healing in Novel Bacteria-based Self-healing Concrete. Cement and Concrete Composites, 2011, 33(7): 763-770 J]

[13] Hsu C, Huang Y, Nimje V, et al. . Comparative Study on the Sand Bioconsolidation Through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis. Crystals., 2018, 8(5): 189 J]

[14] Wang J, Jonkers HM, Boon N, et al. . Bacillus Sphaericus LMG 22257 is Physiologically Suitable for Self-healing Concrete. Applied Microbiology and Biotechnology., 2017, 101(12): 5 101-5 114 J]

[15] Sumathi A, Murali G, Gowdhaman D, et al. . Development of Bacterium for Crack Healing and Improving Properties of Concrete under Wet - Dry and Full-Wet Curing. Sustainability, 2020, 12(24): 10 346 J]

[16] Chen H, Qian C, Huang H. Self-healing cementitious materials based on bacteria and nutrients immobilized respectively. Construction and Building Materials, 2016, 126: 297-303 J]

[17] Zhang YS, Liu Y, Sun XD, et al. . Application of Microbially Induced Calcium Carbonate Precipitation (MICP) Technique in Concrete Crack Repair: A Review. Construction and Building Materials., 2024, 411: 134 313 J]

[18] Reyad AM, Mokhtar G. Impact of the Immobilized Bacillus Cereus MG708176 on the Characteristics of the Bio-based Self-healing Concrete. Scientific Reports., 2023, 13(1): 500 J]

[19] Shi H, Tan L, Du Q, et al. . Green Synthesis of Fe₃O₄ Nanoparticles with Controlled Morphologies Using Urease and Their Application in Dye Adsorption. Dalton Transactions, 2014, 43(33): 12 474-12 479 J]

[20] Miot J, Remusat L, Duprat E, et al. . Fe Biomineralization Mirrors Individual Metabolic Activity in a Nitrate-dependent Fe (II)-oxidizer. Frontiers in microbiology, 2015, 6: 879 J]

[21] Azima M, Bundur ZB. Influence of Sporasarcina Pasteurii Cells on Rheological Properties of Cement Paste. Construction and Building Materials, 2019, 225: 1 086-1 097 J]

[22] DeJong JT, Fritzges MB, Nüsslein K. Microbially Induced Cementation to Control Sand Response to Undrained Shear. Journal of geotechnical and geoenvironmental engineering, 2006, 132(11): 1 381-1 392 J]

[23] Lim YC, Lin SK, Ju YR, et al. . Reutilization of Dredged Harbor Sediment and Steel Slag by Sintering as Lightweight Aggregate. Process Safety and Environmental Protection, 2019, 126: 287-296 J]

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