Effects of Adsorption of Albumin and Gamma-Globulin on the Tribological Performance of a Diamond-Like Carbon Film

Abstract

The effects of surface adsorption of bovine serum albumin (BSA) and human gamma-globulin (HGG) on the tribological performance of a DLC film were investigated using a quartz crystal microbalance with dissipation (QCM-D), a ball-on-disk reciprocating tribometer, and a three-electrode electrochemical cell. The results showed that the wear depth in the BSA solution was higher than that in the HGG solution. In the HGG solution, the HGG-adsorbed layer could act as a lubricating layer and protect the DLC film from wear. The wear volume of DLC film in BSA and HGG mixture solution was higher than that in single HGG solution. This may be because the BSA molecules inhibit the formation of HGG adsorbed layer during sliding.

Keywords

Diamond like carbon (DLC) / Quartz crystal microbalance with dissipation (QCM-D) / Adsorbed layer / Wear

References

[1] Leah E G, Bryan W P, Thomas R T, et al. Biochemical Analyses of Human Osteoarthritic and Periprosthetic Synovial Fluid [J]. Proc. IMechE. Part H: J. Engineering in Medicine, 2014, 228(2): 127-139.

[2] Subir G. Lubricating Ability of Albumin and Globulin on Artificial Joint Implants: a Tribological Perspective [J]. Int. J. Surface Science and Engineering, 2016, 10(2): 193-206.

[3] Subir G, Dipankar C, Nabangshu S D, et al. Tribological Role of Synovial Fluid Compositions on Artificial Joints-a Systematic Review of the Last 10 Years [J]. Lubrication Science, 2014, 26: 387-410.

[4] Trunfio-Sfarghiu A M, Berthier Y, Meurisse M H, et al. Multiscale Analysis of the Tribological Role of the Molecular Assemblies of Synovial Fluid [J]. Case of a Healthy Joint and Implants. Tribology International, 2007, 40(10-12): 1500-1515.

[5] Subir G, Dipankar C, Taposh R, et al. Tribological Performance of the Biological Components of Synovial Fluid in Artificial Joint Implants [J]. Sci. Technol. Adv. Mater., 2015, 16: 045 002

[6] Gispert M P, Serro A P, Colaco R, et al. Friction and Wear Mechanisms in Hip Prosthesis: Comparison of Joint Materials Behaviour in Several Lubricants [J]. Wear, 2006, 260(1): 149-158.

[7] Cooke A, Dowson D, Wright V. The Rheology of Synovial Fluid and Some Potential Synthetic Lubricants for Degenerate Synovial Joints [J]. Engineering in Medicine, 1978, 7(2): 66-72.

[8] Fan J, Myant C W, Underwood R, et al. Inlet Protein Aggregation: a New Mechanism for Lubricating Film Formation with Model Synovial Fluids [J]. Proc. IMechE Part H: J. Engineering in Medicine, 2011, 225(7): 696-709.

[9] C M, Underwood R, Fan J, et al. Lubrication of Metal-on-Metal Hip Joints: the Effect of Protein Content and Load on Film Formation and Wear [J]. Journal of the Mechanical Behavior of Biomedical Materials, 2012, 6: 30-40.

[10] Midathada A, Sk F A, Jin W Y, et al. Tribological Performance of Hydrophilic Diamond-Like Carbon Coatings on Ti-6Al-4V in Biological Environment [J]. Diamond & Related Materials, 2010, 19: 300-304.

[11] Heuberger M P, Widmer M R, Zobeley E, et al. Protein-Mediated Boundary Lubrication in Arthroplasty [J]. Biomaterials, 2005, 26: 1165-1173.

[12] Hauert R. A Review of Modified DLC Coatings for Biological Applications [J]. Diamond and Related Materials, 2003, 12: 583-589.

[13] Salgueiredo E, Vila M, Silva M A, et al. Biocompatibility Evaluation of DLC-Coated Si3N4 Substrates for Biomedical Applications [J]. Diamond & Related Materials, 2008, 17: 878-881.

[14] Love C A, Cook R B, Harvey T J, et al. Diamond Like Carbon Coatings for Potential Application in Biological Implants-a Review [J]. Tribology International, 2013, 63: 141-150.

[15] Roy M E, Whiteside L A, Xu J, et al. Diamond-Like Carbon Coatings Enhance the Hardness and Resilience of Bearing Surfaces for Use in Joint Arthroplasty [J]. Acta Biomaterialia, 2010, 6: 1619-1624.

[16] Sullivan S J L, Topoleski L D T. Surface Modifications for Improved Wear Performance in Artificial Joints: A Review [J]. JOM, 2015, 67(11): 2502-2517.

[17] Zhang T F, Deng Q Y, Liu B, et al. Wear and Corrosion Properties of Diamond Like Carbon (DLC) Coating on Stainless Steel, CoCrMo and Ti6Al4V Substrates [J]. Surf. Coat. Technol., 2015, 273: 12-19.

[18] Wu B J, Deng Q Y, Leng Y X, et al. Characterization of Adsorption and Lubrication of Synovial Fluid Proteins and HA on DLC Joint Bearings Surface [J]. Surf. Coat. Technol., 2017, 320: 320-332.

[19] Liu J, Wang X, Wu B J, et al. Tribocorrosion Behavior of DLC-Coated CoCrMo Alloy in Simulated Biological Environment [J]. Vacuum, 2013, 92: 39-43.

[20] Yan Y, Neville A, Dowson D, et al. Effect of Metallic Nanoparticles on the Biotribocorrosion Behaviour of Metal-on-Metal Hip Prostheses [J]. Wear, 2009, 267: 683-688.

[21] Yan Y, Neville A, Dowson D. Tribo-Corrosion Properties of Cobalt-Based Medical Implant Alloys in Simulated Biological Environments [J]. Wear, 2007, 263: 1105-1111.

[22] Vermeer A, Bremer M, Noede W. Structural Changes of IgG Induced by Heat Treatment and by Adsorption onto a Hydrophobic Teflon Surface Studied by Circular Dichroism Spectroscopy Biochim [J]. Biophys. Acta., 1998, 1(425): 1-12.

[23] Sethuraman A, Han M, Kane R S, et al. Effect of Surface Wettability on the Adhesion of Proteins [J]. Langmuir., 2004, 20: 7779-7788.

[24] Wittemann A, Ballauff M. Secondary Structure Analysis of Proteins Embedded in Spherical Polyelectrolyte Brushes by FT-IR Spectroscopy [J]. Anal. Chem., 2004, 76: 2813-2819.

[25] Nakashima K, Sawae Y, Murakami T. Influence of Protein Conformation on Frictional Properties of Poly (Vinyl Alcohol) Hydrogel for Artificial Cartilage [J]. Tribology Letters, 2007, 26(2): 145-151.

[26] Barnthip N, Parhi P, Golas A, et al. Volumetric Interpretation of Protein Adsorption: Kinetics of Protein-Adsorption Competition from Binary Solution [J]. Biomaterials, 2009, 30: 6495-6513.

[27] Vogler E A. Protein Adsorption in Three Dimensions [J]. Biomaterials, 2012, 33: 1201-1237.