Fabrication and Characterization of One Interpenetrating Network Hydrogel Based on Sodium Alginate and Polyvinyl Alcohol

Abstract

One interpenetrating network hydrogel based on sodium alginate (SA) and polyvinyl alcohol (PVA) was synthesized by combining the raw materials of PVA and SA with the double physical crosslinking methods of freezing thawing and Ca²⁺ crosslinking. The PVA-SA composite hydrogel have been characterized by scanning electron microscopy for surface morphology, infrared spectroscopy for investigating the chemical interactions between PVA and SA, X-ray diffraction for studying the PVA-SA composite structure property and thermal gravimetric for understanding the PVA-SA composite thermal stability. The swelling behavior and the degradation rate of the PVA-SA composite hydrogel were studied in simulated gastrointestinal fluid. Using bovine serum albumin (BSA) and salicylic acid as the model drugs, the release behavior of the PVA-SA composite hydrogel on macromolecular protein drugs and small molecule drug were evaluated. The results showed that the water absorption and degradation ability of the PVA-SA composite hydrogel was much better compared to the pure SA hydrogel or pure PVA hydrogel. The hydrogel exhibited remarkable pH sensitivity and the network was stable in the simulated intestinal fluid for more than 24 h. With the advantages such as mild preparation conditions, simple method, less reagent and none severe reaction, the PVA-SA composite hydrogel is expected to be a new prosperous facile sustained drug delivery carrier.

Keywords

composite hydrogel / polyvinyl alcohol / sodium alginate / drug release behavior

References

[1] Ooi S Y, Ahmad I, Amin M C I M. Cellulose Nanocrystals Extracted from Rice Husks as a Reinforcing Material in Gelatin Hydrogels for Use in Controlled drug Delivery Systems[J]. Ind. Crop. Prod., 2016, 93: 227-234.

[2] Yang J A, Yeom J, Hwang B W, et al. In Situ-forming Injectable Hydrogels for Regenerative Medicine[J]. Prog. Polym. Sci., 2014, 39(12): 1 973-1 986.

[3] Tomme S R V, Storm G, Hennink W E. In Situ Gelling Hydrogels for Pharmaceutical and Biomedical Applications[J]. Int. J. Pharm., 2008, 355(1): 1-18.

[4] Huynh D P, Nguyen M K, Pi BS, et al. Functionalized Injectable Hydrogels for Controlled Insulin Delivery[J]. Biomaterials, 2008, 29(16): 2 527-2 534.

[5] Peppas N A, Bures P, Leobandung W, et al. Hydrogels in Pharmaceutical Formulations[J]. Eur. J. Pharm. Biopharm., 2000, 50(1): 27-46.

[6] Gupta P, Vermani K, Garg S. Hydrogels: From Controlled Release to pH-responsive drug Delivery[J]. Drug Discov. Today, 2002, 7(10): 569-579.

[7] Hoffman A S. Hydrogels for Biomedical Applications[J]. Adv. Drug Deliver. Rev., 2002, 54(1): 3-12.

[8] Hendrawan Khoerunnisa F, Sonjaya Y, et al. Physical and Chemical Characteristics of Alginate-Poly (Vinyl Alcohol) Based Controlled Release Hydrogel[J]. Chem. Eng. J., 2016, 4(4): 4 863-4 869.

[9] Chiellini E, Corti A, D’Antone S, et al. Biodegradation of Poly (Vinyl Alcohol) Based Materials[J]. Prog. Polym. Sci., 2003, 28(6): 963-1 014.

[10] Liu X J, Ren X Y, Guan S, et al. Highly Stretchable and Tough Double Network Hydrogels Via Molecular Stent[J]. Eur Polym. J., 2015, 73: 149-161.

[11] Lozinsky V I, Galaev I Y, Plieva F M, et al. Polymeric Cryogels as Promising Materials of Biotechnological Interest[J]. Trends Biotechnol., 2003, 21(10): 445-451.

[12] Zhao Y, Shen W, Chen Z G, et al. Freeze-thaw Induced Gelation of Alginates[J]. Carbohyd. Polym., 2016, 148: 45-51.

[13] Huang H B, Yao J L, Qin H T, et al. Conducting Hydrogels Originating from High-pressure Induced Gelation of Poly(Vinyl Alcohol) and in-situ Polymerization of Aniline[J]. Synthetic metals, 2016, 221: 15-18.

[14] Nagura M, Hamano T, Ishikawa H. Structure of Poly(Vinyl Alcohol) Hydrogel Prepared by Repeated Freezing and Melting[J]. Polymer, 1989, 30(4): 762-765.

[15] Dini L, Panzarini E, Miccoli M A, et al. In Vitro Study of the Interaction of Polyalkilimide and Polyvinyl Alcohol Hydrogels with Cells[J]. Tissue Cell, 2005, 37(6): 479-487.

[16] Bodugoz-Senturk H, Choi J, Ebru O, et al. The Effect of Polyethylene Glycol on the Stability of Pores in Polyvinyl Alcohol Hydrogels during an Nealing[J]. Biomaterials, 2008, 29(2): 141-149.

[17] Hu Y, Chen T, Dong X Y, et al. Preparation and Characterization of Composite Hydrogel Beads Based on Sodium Alginate[J]. Polym. Bull., 2015, 72(11): 2 857-2 869.

[18] Tsai F H, Chiang P Y, Kitamura Y. Producing Liquid-core Hydrogel Beads by Reverse Spherification: Effect of Secondary Gelation on Physical Properties and Release Characteristics[J]. Food Hydrocolloid, 2017, 62: 140-148.

[19] George M, Abraham T E. Polyionic Hydrocolloids for the Intestinal Delivery of Protein Drugs: Alginate and Chitosan-a Review[J]. J. Controlled Release, 2006, 114(1): 1-14.

[20] Lonita M, Pandele M A, Lovu H. Sodium Alginate/Graphene Oxide Composite Films with Enhanced Thermal and Mechanical Properties[J]. Carbohydr. Polym., 2013, 94(1): 339-344.

[21] Fan M, Ma Y, Tan H P. Covalent and Injectable Chitosan-Chondroitin Sulfate Hydrogels Embedded with Chitosan Microspheres for Drug Delivery and Tissue Engineering[J]. Mat. Sci. Eng.: C, 2017, 71: 67-74.

[22] Abureesh M A, Oladipo A A, Gazi M. Facile Synthesis of Glucose-sensitive Chitosan-Poly(vinyl alcohol) hydrogel: Drug Release Optimization and Swelling Properties[J]. Int. J. Biol. Macromol., 2016, 90: 75-80.

[23] Constantin M, Bucatariu SM, Doroftei F, et al. Smart Composite Materials Based on Chitosan Microspheres Embedded in Thermosensitive Hydrogel for Controlled Delivery of Drugs[J]. Carbohyd. Polym., 2017, 157: 493-502.

[24] Snigdha K, Singh B K, Mehta A S, et al. Self-assembling N-(9-Fluorenylmethoxycarbonyl)-l-Phenylalanine Hydrogel as Novel Drug Carrier[J]. Int. J. Biol. Macromol., 2016, 93: 1 639-1 646.

[25] Qi X L, Hu X Y, Wei W, et al. Investigation of Salecan/Poly(Vinyl Alcohol) Hydrogels Prepared by Freeze/Thaw Method[J]. Carbohyd. Polym., 2015, 118: 60-69.

[26] Ashraf M U, Hussain M A, Muhammad G, et al. A Superporous and Superabsorbent Glucuronoxylan Hydrogel from Quince (Cydonia Oblanga): Stimuli Responsive Swelling, on-off Switching and Drug Release[J]. Int. J. Biol. Macromol., 2017, 95: 138-144.

[27] Thakur S, Pandey S, Arotiba O A. Development of a Sodium Alginate-based Organic/Inorganic Superabsorbent Composite Hydrogel for Adsorption of Methylene Blue[J]. Carbohyd. Polym., 2016, 153: 34-46.

[28] Huang B, Liu M X, Long Z, et al. Effects of Halloysite Nanotubes on Physical Properties and Cytocompatibility of Alginate Composite Hydrogels. Mat. Sci. Eng.: C, 2017, 70: 303-310.

[29] Gong X, Branford-White C, Tao L, et al. Preparation and Characterization of a Novel Sodium Alginate Incorporated Self-assembled Fmoc-FF Composite Hydrogel[J]. Mat. Sci. Eng.: C, 2016, 58(9): 478-486.

[30] Singh B, Sharma V. Crosslinking of Poly(Vinylpyrrolidone)/Acrylic Acid with Tragacanth Gum for Hydrogels Formation for Use in Drug Delivery Applications[J]. Carbohyd. Polym., 2017, 157: 185-195.

[31] Oladipo A A, Gazi M, Saber-Samandari S. Adsorption of Anthraquinone Dye onto Eco-friendly Semi-IPN Biocomposite Hydrogel: Equilibrium Isotherms, Kinetic Studies and Optimization[J]. J. Taiwan Inst. Chem. E, 2014, 45(2): 653-664.

[32] Wang L, Liu M Z, Gao C M, et al. A pH-, Thermo-, and Glucose-, Triple-responsive Hydrogels: Synthesis and Controlled Drug Delivery[J]. React. Funct. Polym., 2010, 70(3): 159-167.

[33] Nayak AK, Kalia S, Hasnain MS. Optimization of Aceclofenac-loaded Pectinate-Poly(Vinyl Pyrrolidone) Beads by Response Surface Methodology[J]. Int. J. Biol. Macromol., 2013, 62(11): 194-202.