Synthesis of PEI-Functionalized Magnetic Nanoparticles for Capturing Bacteria

Abstract

Polyethyleneimine (PEI) functionalized Fe₃O₄ MNPs were synthesized by a modified hypothermal oxidative hydrolysis method. The magnetic nanoparticles showed positively charged surface, strong magnetic responsivity and uniform particle size distribution at 56.1±0.6 nm. Aggregation of these magnetic nanoparticles were observed on the surface of different type of bacteria. Magnetic capturing of bacteria were facilitated by these magnetic nanoparticles. The capturing efficiency could reach 90% after two rounds of interactions of 5 minutes. The mechanism and process of interactions between bacteria and polyethyleneimine functionalized Fe₃O₄ magnetic nanoparticles were explored and discussed. The present study not only provides insight into interactions between Fe₃O₄@PEI MNPs and bacterial cells, but also opens a new avenue for designing and applying Fe₃O₄@PEI MNPs as biosensors in microbiology, medicine, and environmental science.

Keywords

magnetic nanoparticles (MNPs) / polyethyleneimine (PEI) / magnetic separation / bacterial surface

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