Fabrication and Characterization of 3D Graded PDMS Scaffolds Using Vacuum-Assisted Resin Transfer Moulding

Abstract

Graded porous scaffold can be applied to study the interactions between cells and scaffold with different pore sizes. Polydimethylsiloxane (PDMS) scaffold with an axial pore size grade was successfully manufactured via vacuum-assisted resin transfer moulding (VARTM) and particle leaching technologies. The properties of graded PDMS scaffolds, including porosity, water absorption, interconnectivity, compression modulus, as well as compression strength were investigated. The results showed that the smaller the size of the NaCl particles is, the higher the porosity and water absorption of graded PDMS scaffolds would be. The graded PDMS scaffold fabricated had a compressive modulus and a compressive strength of 19.69±1.42 kPa and 4.76±0.22 kPa, respectively. Moreover, the graded chitosan (CS)-coated PDMS scaffolds were prepared by using dip-coating technique under low vacuum and their hydrophilicity was examined. It is found that the water contact angle (WCA) will decrease with an increase in the CS solution concentration and the coating time, which indicates that CS-coated PDMS scaffolds exhibit noticeable hydrophilicity compared with graded PDMS scaffold.

Keywords

polydimethylsiloxane (PDMS) / graded porous scaffold / biomedical applications

References

[1] Flemming R G, Murphy C J, Abrams G A, et al. Effects of Synthetic Micro-and Nano-Structured Surfaces on Cell Behavior[J]. Biomaterials, 1999, 20: 573-588.

[2] Miao X G, Sun D. Graded/Gradient Porous Biomaterials[J]. Materials, 2010, 3(1): 26-47.

[3] Gong X, Tang C Y, Zhang Y, et al. Fabrication of Graded Macroporous Poly(Lactic Acid) Scaffold by a Progressive Solvent Casting/Porogen Leaching Approach[J]. J. Appl. Polym. Sci., 2012, 125(1): 571-577.

[4] Tang Y, Zhao K, Hu L, et al. Two-Step Freeze Casting Fabrication of Hydroxyapatite Porous Scaffolds with Bionic Bone Graded Structure[J]. Ceram. Int., 2013, 39(8): 9703-9707.

[5] Yin D, Wu H, Liu C, et al. Fabrication of Composition-Graded Collagen/Chitosan-Polylactide Scaffolds with Gradient Architecture and Properties[J]. React. Funct. Polym., 2014, 83(756): 399-412.

[6] Salerno A, Iannace S, Netti P A. Graded Biomimetic Osteochondral Scaffold Prepared via CO₂ Foaming and Micronized NaCl Leaching[J]. Mater. Lett., 2012, 82: 137-140.

[7] Si J H, Cui Z X, Xie P, et al. Characterization of 3D Elastic Porous Polydimethylsiloxane (PDMS) Cell Scaffolds Fabricated by VARTM and Particle Leaching[J]. J. Appl. Polym. Sci., 2015, 133: 42909.

[8] Paglicawan M A, Kin B S, Blessie-Basilia A B, et al. Plasma-Treated Abaca Fabric/Unsaturated Polyester Composite Fabricated by Vacuum-Assisted Resin Transfer Molding[J]. Int. J. Pr. Eng. Man-GT., 2014, 1(3): 241-246.

[9] Pedraza E, Brady A C. Synthesis of Macroporous Poly(Dimethylsiloxane) Scaffolds for Tissue Engineering Applications[J]. J. Biomat. Sci.-Polym. E., 2013, 24(9): 1041-1056.

[10] Hassler C, Boretius T, Stieglitz T. Erratum: Polymers for Neural Implants[J]. J. Polym. Sci. Polym. Phys., 2011, 49(3): 18-33.

[11] Xu F, Yin M, Ding H F, et al. Evaluation of Apatite-Coated Chitosan Microspheres for Bone Regeneration[J]. J. Wuhan Univ. Technol.-Mater. Sci. Ed., 2014, 29(2): 391-397.

[12] Domard A. A Perspective on 30 Years Research on Chitin and Chitosan[J]. Carbohyd. Polym., 2011, 84(2): 696-703.

[13] Gong X, Tang C Y, Wong C T, et al. Fabrication of Poly(Lactic Acid) Scaffolds by a Modified Solvent Casting/Porogen Leaching Method[J]. e-Polymers, 2010, 10(1): 1264-1272.

[14] Yang Y, Zhao J, Zhao Y, et al. Formation of Porous PLGA Scaffolds by a Combining Method of Thermally Induced Phase Separation and Porogen Leaching[J]. J. Appl. Polym. Sci., 2008, 109(2): 1232-1241.

[15] Wen P, Gao J, Zhang Y, et al. Fabrication of Chitosan Scaffolds with Tunable Porous Orientation Structure for Tissue Engineering[J]. J. Biomat. Sci.-Polym. E, 2010, 22(1-3): 19-40.

[16] Oh S H, Park I K, Kim J M, et al. In Vitro and in Vivo Characteristics of PCL Scaffolds with Pore Size Gradient Fabricated by a Centrifugation Method[J]. Biomaterials, 2007, 28(9): 1664-1671.

[17] Cui W G, Cheng L Y, Li H Y, et al. Preparation of Hydrophilic Poly(L-Lactide) Electrospun Fibrous Scaffolds Modified with Chitosan for Enhanced Cell Biocompatibility[J]. Polymer, 2012, 53(11): 2298-2305.