Cytocompatibility evaluation of grafted IKVAV PLEOF hydrogels with bone marrow mesenchymal stem cells

Abstract

The novel hydrogels-grafted IKVAV poly (lactide-co-ethylene oxide-co-fumarate) (PLEOF) hydrogels (GIPHs) were developed. The rat bone marrow mesenchymal stem cells (BMMSCs) were employed, and the cell vitality and apoptosis assays were carried out to evaluate the cytocomptibility of GIPHs. Our data demonstrated that the influence of GIPHs on the proliferation of BMMSCs was in a concentration and time dependent manner. The proliferative ability of BMMSCs in GIPHs-treated group (100 μg/mL) after 72 h presented a maximum response which was 30.1% more than that of control group. The numbers of apoptotic cells in GIPHs-treated group (100 μg/mL) were just as much as that of control group after 24 h treatment. The GIPHs are able to provide an appropriate environment for BMMSCs survival and proliferation.

Keywords

proliferation / IKVAV / hydrogels / vitality / apoptosis

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