Anti-biofilm effect of glass ionomer cements incorporated with chlorhexidine and bioactive glass

Abstract

The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated. The experimental results showed that groups incorporated with 1% chlorhexidine exhibited a significant reduction of optical density values of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm. However, groups incorporated with 10% bioactive glass did not affect the optical density values and the biofilm formation. The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine. Nevertheless, the compressive strength was lower when the materials were incorporated with bioactive glass. It can be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early S mutans biofilm formation. Controlled release/sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass.

Keywords

glass ionomer cements / biofilm / chlorhexidine / bioactive glass

References

[1] Theilade J. Dental Plaque and Dental Calculus[M]. In: Lindhe J (ed.) Clinical Periodontology, 2nd ed. Munksgaard, Copenhagen, 1989: 92–128

[2] Loesche W.J. Role of Streptococcus Mutans in Human Dental Decay[J]. Microbiol Rev., 1986, 50: 353-80.

[3] Burke F.I.T., Wilson N.H.F., Cheung S.W., Mjor I.A. Influence of Patient Factors on Age of Restorations — A Failure and Reasons for their Placement and Replacement[J]. J. Dent., 2001, 29: 317-24.

[4] Fujii B. Katsuyama S., Ishikawa T., Fujii B. The Development of Glass Ionomer Cement[M]. Glass Ionomer Dental Cement-the Materials and their Clinical Use, 1993 Tokyo Ishiyaku Euro America Inc. 7-14.

[5] Imazato S. Bio-active Restorative Materials with Antibacterial Effects: new Dimension of Innovation in Restorative Dentistry[J]. Dent. Mater. J., 2009, 28: 11-19.

[6] Türkün L. S., Türkün M., Erturul F., Ateş M., Brugger S. Long-term Antibacterial Effects and Physical Properties of a Chlorhexidine-containing Glass Ionomer Cement[J]. J. Esthet. Restor.Dent., 2008, 20: 29-44.

[7] Charles C.H., Mostler K.M., Bartels L.L., Mankodi S.M. Comparative Antiplaque and Antigingivitis Effectiveness of a Chlorhexidine and an Essential Oil Mouthrinse: 6-month Clinical Trial[J]. J. Clin. Periodontol., 2004, 31: 878-84.

[8] Xie Z. P., Zhang C. Q., Yi C. Q., Qiu J. J., Wang J. Q., Zhou J. In Vivo Study Effect of Particulate Bioglass® in the Prevention of Infection in Open Fracture Fixation[J]. J. Biomed Mater. Res. B, Appl. Biomater., 2009, 90: 195-201.

[9] Allan I., Newman H., Wilson M. Antibacterial Activity of Particulate Bioglass Against Supra- and Subgingival Bacteria[J]. Biomaterials., 2001, 22: 168 3-168 7.

[10] Zhang D., Munukka E., Hupa L., . Factor Controlling Antibacterial Properties of Bioactive Glasses[J]. Key Eng. Mater., 2007, 330–332: 173-176.

[11] Waltimo T., Brunner T. J., Vollenweider M., Stark W. J., Zehnder M. Antimicrobial Effect of Nanometric Bioactive Glass 45S5[J]. J. Dent. Res., 2007, 86: 754-757.

[12] Waltimo T., Mohn D., Paqué F., Brunner T. J., Stark W. J., Imfeld T., Schätzle M., Zehnder M. Fine-tuning of Bioactive Glass for Root Canal disinfection[J]. J. Dent. Res., 2009, 88: 235-8.

[13] Hu S., Chang J., Liu M., Ning C. Study on Antibacterial Effect of 45S5 Bioglass®[J]. J. Mater. Sci. Mater. Med., 2009, 20: 281-286.

[14] Young A. M. FTIR Investigation of Polymerisation and Polyacid Neutralisation Kinetics in Resin-modified Glass-ionomer Dental Cements[J]. Biomaterials, 2002, 23: 328 9-329 5.

[15] Namba N., Yoshida Y., Nagaoka N., Takashima S., Matsuura-Yoshimoto K., Maeda H., Van Meerbeek B., Suzuki K., Takashiba S. Antibacterial Effect of Bactericide Immobilized in Resin Matrix[J]. Dent. Mater., 2009, 25: 424-30.

[16] Ribeiro J., Ericson D. In Vitro Antibacterial Effect of Chlorhexidine Added to Glass-ionomer Cements[J]. Scand. J. Dent. Res., 1991, 99: 533-540.

[17] Palmer G., Jones F. H., Billington R. W., Pearson G. J. Chlorhexidine Release from an Experimental Glass Ionomer Cement[J]. Biomaterials, 2004, 25: 542 3-543 1.

[18] Takahashi Y., Imazato S., Kaneshiro A. V., Ebisu S., Frencken J. E., Tay F. R. Antibacterial Effects and Physical Properties of Glass-ionomer Cements Containing Chlorhexidine for the ART Approach[J]. Dent. Mater., 2006, 22: 647-652.

[19] Imazato S. Antibacterial Properties of Resin Composites and Dentin Bonding Systems[J]. Dent. Mater., 2003, 19: 449-457.

[20] Palenik C.J., Behnen M.J., Setcos J.C., Miller C.H. Inhibition of Microbial Adherence and Growth by Various Glass Ionomers in Vitro[J]. Dent. Mater., 1991, 8: 16-20.

[21] Barkhordar R.A., Kempler D., Pelzer R.R.B., Stark M.M. Antimicrobial Action of Glass-ionomer Lining Cement on the S Sanguis and S. mutans[J]. Dent. Mater., 1989, 5: 281-282.

[22] Yli-Urpo H., Vallittu P. K., Närhi T. O., Forsback A.-P., Väkiparta M. Release of Silica, Calcium, Phosphorus, and Fluoride from Glass Ionomer Cement Containing Bioactive Glass[J]. J Biomater. Appl., 2004, 19: 5-20.

[23] Gubler M., Brunner T. J., Zehnder M., Waltimo T., Sener B., Stark W. J. Do Bioactive Glasses Convey a Disinfecting Mechanism Beyond a Mere Increase in pH?[J]. Int. Endod. J., 2008, 41: 670-8.

[24] Ellakuria J., Triana R., Mínguez N., Soler I., Ibaseta G., Maza J., García-Godoy F. Effect of One-year Water Storage on the Surface Microhardness of Resin-modified Versus Conventional Glass-ionomer Cements[J]. Dent. Mater., 2003, 19: 286-90.

[25] Yli-Urpo H., Lassila L.V., Närhi T., Vallittu P.K. Compressive Strength and Surface Characterization of Glass Ionomer Cements Modified by Particles of Bioactive Glass[J]. Dent. Mater., 2005, 21: 201-209.

[26] Guo J., Pan Q., Huang C., Zhao Y., Ouyang X., Duan S. The Role of Surfactant and Costabilizer in Controlling Size of Nanocapsules Containing TEGDMA in Miniemulsion[J]. J. Wuhan Univ. Technol., 2009, 24: 1 004-1 006.