Effects of β-TCP ceramics on osteoblast cellular proliferating, mineralization and osteocalcin expression

Zhitao Qi; Qihuan Zhang; Qiang Zheng; Honglian Dai; Zisheng Wang; Ming Qiu; Shipu LI

doi:10.1007/s11595-012-0417-3

Journal of Wuhan University of Technology Materials Science Edition ›› 2012, Vol. 27 ›› Issue (1) : 107 -109. DOI: 10.1007/s11595-012-0417-3

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Effects of β-TCP ceramics on osteoblast cellular proliferating, mineralization and osteocalcin expression

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Abstract

After co-cultrured osteoblast with β-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that β-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that β-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that β-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

Keywords

β-TCP ceramics / osteoblast / cellular proliferating / mineralization / osteocalcin

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Zhitao Qi, Qihuan Zhang, Qiang Zheng, Honglian Dai, Zisheng Wang, Ming Qiu, Shipu LI. Effects of β-TCP ceramics on osteoblast cellular proliferating, mineralization and osteocalcin expression. Journal of Wuhan University of Technology Materials Science Edition, 2012, 27(1): 107-109 DOI:10.1007/s11595-012-0417-3

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