Effects of β-TCP ceramics on intracellular Ca²⁺ concentration, mineralization of osteoblast and protein structure

Abstract

β-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of β-TCP ceramics on intracellular Ca²⁺ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that β-TCP could increase the intracelluar Ca²⁺ concentration and mineralization of osteoblast, indicating that β-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, β-TCP ceramics could increase the content of α-helix and β-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after β-TCP ceramics implanted.

Keywords

β-TCP ceramics / mineralization / osteoblast / [Ca²⁺] _i / protein structure

References

[1] Weinand C., Pomerantseva I., Neville C.M., . Hydrogel-β-TCP Scaffolds and Stem Cells for Tissue Engineering Bone [J]. Bone, 2006, 38: 555-563.

[2] Lu B., Lu X.F., Zhang Z., . The Long Term Study of HA/TCP Biphasic Ceramics’ Osteoinduction [J]. Key. Eng. Mater., 2005, 288–289: 237-240.

[3] Burchardt H. The Biology of Bone Graft Repair [J]. Clin. Orthop. Relat. Res., 1983, 174: 28-42.

[4] Cao H., Kuboyama N. A Biodegradable Porous Composite Scaffold of PGA/β-TCP for Bone Tissue Engineering [J]. Bone, 2010, 46: 386-395.

[5] Chevalier J., Gremillard L. Ceramics for Medical Applications: A Picture for the Next 20 Years [J]. Eur. Ceram. Soc., 2009, 29: 1245-1255.

[6] Oonishi H., Hench L.L., Wilson J., . Compatative Bone Growth Behavior in Granules of Bioceramic Materials of Various Sizes [J]. J. Biomed. Mater. Res. Part B, 1999, 44: 31-43.

[7] Kondo N., Ogose A., Tokunaga K., . Bone Formation and Resorption of Highly Purified Beta-tricalcium Phosphate in the Rat Femoral Condyle [J]. Biomaterials, 2005, 26: 5600-5608.

[8] Qi Z.T., Dai H.L., Zhang Q.H., . Effect of Beta-TCP Ceramic on the Total Protein of Osteoblasts [J]. J. of Wuhan. Univ. of Technol., 2007, 22: 98-101.

[9] Yuan H., Van Den Doel M., Li S., . A Comparison of the Osteoinductive Potential of Two Calcium Phosphate Ceramics Implanted Intramuscularly in Goats [J]. J. Mater. Sci. Mater. Med., 2002, 13: 1271-1275.

[10] Ye F., Lu X.F., Lu B., . A Pong-term Evalution of Osteoinductive HA/β-TCP Ceramics in vivo: 4.5 Years Study in Pigs [J]. J. Mater. Sci. Mater. Med., 2007, 18: 2173-2178.

[11] Narita H., Itoh S., Imazato S., . An Explanation of the Mineralization Mechanism in Osteoblast Induced by Calcium Hydroxide [J]. Acta. Biomaterialia., 2010, 6: 586-590.

[12] Xie J., Riley C., Kumar M., . FT-IR/ATR Study of Protein Absorption and Brushite Transformation to Hydroxyapatite [J]. Biomaterials, 2002, 23: 3 609-3 616.

[13] Johnsson M.S.A., Paschalis E., Nancollas G.H. Davies J.E. Kinetics of Mineralization, Demineralization, and Transformation of Calcium Phosphates at Mineral and Protein Surfaces [M]. The Bone-biomaterial Interface, 1991 Toronto University of Toronto Press 68-75.

[14] Lu Z., Zreiqat H. Beta-tricalcium Phosphate Exerts Osteoconductivity Through a2b1 Integrin and Down-stream MAPK/ERK Signaling Pathway [J]. Biochem. Biophys. Res. Commun., 2010, 394: 323-329.

[15] Marino G., Rosso F., Cafiero G., . Beta-tricalcium Phosphate 3D Scaffold Promote Alone Ostogenic Differentiation of Human Adipose Stem Cells: in vitro Study [J]. J. Mater. Sci. Mater. Med., 2010, 21: 353-363.

[16] Kasten P., van Griensven M., Barkhausen T., . Comparison of Human Bone Marrow Stromal Cells Seeded on Calcium-Deficient Hydroxyapatite, Beta-tricalcium Phosphate and Demineralized Bone Matrix [J]. Biomaterials, 2003, 24: 2 593-2 603.

[17] Neamat A., Gawish A., Gamal-Eldeen A.M. Beta-tricalcium Phosphate Promotes Cell Proliferation, Osteogenesis and Bone Regeneration in Intrabony Defects in Dogs [J]. Arch. Oral. Biol., 2009, 54: 1 083-1 090.