Preparation of immobilized ɛ-polylysine PET nonwoven fabrics and antibacterial activity evaluation

Limei Hao , Zheng Wang , Jiancheng Qi , Shuang Wang , Lili Hou , Jinhui Wu , Jingquan Yang

Journal of Wuhan University of Technology Materials Science Edition ›› 2011, Vol. 26 ›› Issue (4) : 675 -680.

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Journal of Wuhan University of Technology Materials Science Edition ›› 2011, Vol. 26 ›› Issue (4) : 675 -680. DOI: 10.1007/s11595-011-0290-5
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Preparation of immobilized ɛ-polylysine PET nonwoven fabrics and antibacterial activity evaluation

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Abstract

A novel antibacterial material (L-PET) was prepared by immobilizing ɛ-polylysine on polyethylene terephthalate (PET) nonwoven fabrics. Surface modifications of the fabric were performed by using a chemical modification procedure where carboxyl groups were prepared on the PET surface, a coupling agent was grafted, and the ɛ-polylysine was immobilized. Scanning electron microscopy (SEM) was used to analyze the surface morphology of the fabrics, while the toluidine blue method and X-ray photoelectron spectroscopy (XPS) were used to evaluate the grafting densities. The antibacterial activities of the L-PET were investigated by using the shaking-flask method. The electron micrographs showed that the surface of the blank PET and the modified fabrics did not change. The results of XPS analysis confirmed that ɛ-polylysine was successfully grafted onto the surface of PET. The results of the antibacterial experiments showed that L-PET fabrics had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, and that L-PET fabrics were stable in storage for at least two years.

Keywords

PET nonwoven fabric / ɛ-polylysine / immobilization / antibacterial activity / antibacterial material

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Limei Hao, Zheng Wang, Jiancheng Qi, Shuang Wang, Lili Hou, Jinhui Wu, Jingquan Yang. Preparation of immobilized ɛ-polylysine PET nonwoven fabrics and antibacterial activity evaluation. Journal of Wuhan University of Technology Materials Science Edition, 2011, 26(4): 675-680 DOI:10.1007/s11595-011-0290-5

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References

[1]

Dutta P.K., Tripathi S., Mehrotra G.K., . Perspectives for Chitosan Based Antimicrobial Films in Food Applications[J]. Food Chemistry, 2009, 114: 1 173-1 182.

[2]

Beyth N., Houri-Haddad Y., Baraness-Hadar L., . Surface Antimicrobial Activity and Biocompatibility of Incorporated Polyethylenimine Nanoparticles[J]. Biomaterials, 2008, 29: 4 157-4 163.

[3]

Nambaa N., Yoshidab Y., Nagaokac N., . Antibacterial Effect of Bactericide Immobilized in Resin Matrix[J]. Dental Materials, 2009, 25: 424-430.

[4]

Bagheri M., Beyermann M., Dathe M. Immobilization Reduces the Activity of Surface-Bound Cationic Antimicrobial Peptides with No Influence upon the Activity Spectrum[J]. Antimicrob Agents CH., 2009, 53: 1 132-1 141.

[5]

Kito M., Takimoto R., Yoshida T., . Purification and Characterization of an Epsilon-poly-L -Lysine -Degrading Enzyme from an Epsilon-poly-L-lysine-producing Strain of Streptomyces Albulus[J]. Arch Microbiol., 2002, 178: 325-330.

[6]

Yoshida T., Nagasawa T. ɛ-Poly-l-lysine: Microbial Production, Biodegradation and Application Potential[J]. Appl. Microbiol Biotechnol., 2003, 62: 21-26.

[7]

Shih I.L., Van Y.T. The Production of Poly-(Gamma-glutamic acid) from Microorganisms and Its Various Applications[J]. Bioresour Technol., 2001, 79: 207-225.

[8]

Boxus T., Deldime-Rubbens M., Mougenot P., . Chemical Assays of End-groups Displayed on the Surface of Poly (Ethylene Terephthalate) (PET) Films and Membranes by Radiolabeling[J]. Polym Advan Technol., 1999, 7(7): 589-598.

[9]

Chollet C., Chanseau C., Brouillaud B., . RGD Peptides Grafting onto Poly(Ethylene Terephthalate) with Well Controlled Densities[J]. Biomol. Eng., 2007, 24: 477-482.

[10]

GB/T 20944.3-2008. Textiles-Evaluation for Antibacterial Activity-part 3: Shake Flask Method[S].

[11]

Beyth N., Domb A.J., Weiss E.I. An in Vitro Quantitative Antibacterial Analysis of Amalgam and Composite Resins[J]. J. Dent., 2007, 35: 201-206.

[12]

The Ministry of Public Health of the People’s Republic of China. Technical Standard For Disinfection[S]. 2002 Edition

[13]

Chollet C., Chanseau C., Remy M., . The Effect of RGD Density on Osteoblast and Endothelial Cell Behavior on RGD-Grafted Polyethylene Terephthalate Surfaces[J]. Biomaterials, 2009, 30: 711-720.

[14]

Liu Y., Chen J.R., Yang Y., . Improved Bblood Compatibility of Poly (Ethylene Terephthalate) Films Modified with L-arginine[J]. J. Biomater. Sci. Polymer. Ed., 2008, 19: 497-507.

[15]

Nishikawa M., Ogawa K. Distribution of Microbes Producing Antimicrobial ɛ-Poly-L-Lysine Polymers in Soil Microflora Determined by a Novel Method[J]. Appl. Environ. Microb., 2002, 68: 3 575-3 581.

[16]

Shih I.L., Shen M.H., Van Y.T. Microbial Synthesis of Poly (ɛ-lysine) and Its Various Application[J]. Bioresource Technol., 2006, 97: 1 148-1 159.

[17]

Curylo E, Buckholt M A (Professor). Isolation And Antimicrobial Potential of Epsilon-Poly-L-Lysine[D]. Worcester Polytechnic Institute. In Partial Fulfillment of the Requirements for the Degree of Bachelor of Science, April 24, 2008

[18]

Shima S., Matsuoka H., Iwamoto T., . Antimicrobial Action of Epsilon-poly-L-lysine[J]. J. Antibiot., 1984, 37: 1 449-1 455.

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