Preparation and characterization of chitosan/β-GP membranes for guided bone regeneration

Abstract

Bioabsorbable chitosan/β-glycerol phosphate (CS/β-GP) composite membranes were fabricated through a relatively PH neutral and mild sol-gel process for guided bone regeneration (GBR). Their structural properties, morphology, and tensile strength were investigated. FTIR and XRD analyses indicated that there were chemical bonds between the CS andβ-GP. SEM analysis revealed that the CS/β-GP composite membranes had a porous structure both at the surface and in sublayers. Even though the incorporation ofβ-GP in the CS matrix decreased the initial tensile strength of the membrane, the CS/β-GP membranes were still fit for GBR application with their tensile strength of roughly 1 MPa. The concentration ofβ-GP was proportional to the pore size and thickness but was inversely proportional to the tensile strength of the CS/β-GP membrane. The present findings indicate that, based on its characteristics, the CS/β-GP composite membrane is a potential bioresorbable membrane for use in guided bone regeneration.

Keywords

chitosan / β-glycerol phosphate / guided bone regeneration / membrane / thermo-sensitive hydrogel

References

[1] Dahlin C., Sennerby L., Lekholm U., . Generation of New Bone Around Titanium Implants Using a Membrane Technique: an Experimental Study in Rabbits[J]. Int. J. Oral. Maxillofac Impl., 1989, 4(1): 19-25.

[2] Buser D., Dula K., Belser U., Hirt H.P., . Localized Ridge Augmentation Using Guided Bone Regeneration. 1. Surgical Procedure in the Maxilla[J]. Int. J. Periodont. Rest Dent., 1993, 13(1): 29-45.

[3] McAllister B.S., Haghighat K. Bone Augmentation Techniques[ J]. J. Periodontol., 2007, 78(3): 377-396.

[4] Llambés F., Silvestre F.J., Caffesse R. Vertical Guided Bone Regeneration with Bioabsorbable Barriers[J]. J. Periodontol., 2007, 78(10): 2036-2042.

[5] Geurs N.C., Korostoff J.M., Vassilopoulos P.J., . Clinical and Histologic Assessment of Lateral Alveolar Ridge Augmentation Using a Synthetic Long-term Bioabsorbable Membrane and an Allograft[J]. J. Periodontol., 2008, 79(7): 1133-1140.

[6] Kozlovsky A., Aboodi G., Moses O., . Bio-degradation of a Resorbable Collagen Membrane (Bio-Gide) Applied in a Double-layer Technique in Rats[J]. Clin. Oral. Implants Res., 2009, 20(10): 1116-1123.

[7] Elsabee M.Z., Morsi R.E., Al-Sabagh A.M. Surface Active Properties of Chitosan and Its Derivatives[J]. Colloids Surf. B Biointerfaces., 2009, 74(1): 1-16.

[8] Kim I.Y., Seo S.J., Moon H.S., . Chitosan and Its Derivatives for Tissue Engineering Applications[J]. Biotechnol. Adv., 2008, 26(1): 1-21.

[9] Jiang T., Kumbar S.G., Nair L.S., . Biologically Active Chitosan Systems for Tissue Engineering and Regenerative Medicine[J]. Curr. Top. Med. Chem., 2008, 8(4): 354-364.

[10] Kuo S.M., Chang S.J., Chen T.W., . Guided Tissue Regeneration for Using a Chitosan Membrane: an Experimental Study in Rats[J]. J. Biomed. Mater. Res., 2006, 76(2): 408-415.

[11] Teng S.H., Lee E.J., Yoon B.H., . Chitosan/nanohydroxyapatite Composite Membranes via Dynamic Filtration for Guided Bone Regeneration[J]. J. Biomed. Mater. Res. A, 2009, 88(3): 569-580.

[12] Liuyun J., Yubao L., Chengdong X. A Novel Composite Mmembrane of Chitosan-carboxymethyl Cellulose Polyelectrolyte Complex Membrane Filled with Nnano-hydroxyapatite I. Preparation and Properties[J]. J. Mater. Sci. Mater. Med., 2009, 20(8): 1645-1652.

[13] Song S.J., Jeon O., Yang H.S., . Effects of Culture Conditions on Osteogenic Differentiation in Human Mesenchymal Stem Cells[J]. J. Microbiol. Biotechnol., 2007, 17(7): 1113-1119.

[14] Chenite A., Chaput C., Wang D., . Novel Injectable Neutral Solutions of Chitosan Form Biodegradable Gels in Situ[J]. Biomaterials, 2000, 21(21): 2155-2161.

[15] Cho J., Heuzey M.C., Bégin A., . Physical Gelation of Chitosan in the Presence of Beta-glycerophosphate: the Effect of Temperature[J]. Biomacromolecules, 2005, 6(6): 3267-3275.

[16] Ahmadi R., de Bruijn J.D. Biocompatibility and Gelation of Chitosan-glycerol Phosphate Hydrogels[J]. J. Biomed. Mater. Res. A., 2008, 86(3): 824-832.

[17] Ruel-Gariépy E., Chenite A., Chaput C., . Characterization of Thermosensitive Chitosan Gels for the Sustained Delivery of Drugs[J]. Int. J. Pharm., 2000, 203(1–2): 89-98.

[18] Richardson S.M., Hughes N., Hunt J.A., . Human Mesenchymal Stem Cell Differentiation to NP-like Cells in Chitosanglycerophosphate Hydrogels[J]. Biomaterials, 2008, 29(1): 85-93.

[19] Cho M.H., Kim K.S., Ahn H.H., . Chitosan Gel as an in Situ-forming Scaffold for Rat Bone Marrow Mesenchymal Stem Cells in vivo[J]. Tissue Eng. Part A, 2008, 14(6): 1099-1108.

[20] Yan J., Yang L., Wang G., . Biocompatibility Evaluation of Chitosan-based Injectable Hydrogels for the Culturing Mice Mesenchymal Stem Cells in vitro[J]. J. Biomater. Appl., 2010, 24(7): 625-637.

[21] Chung Y.M., Simmons K.L., Gutowska A., . Sol-gel Transition Temperature of PLGA-g-PEG Aqueous Solutions[J]. Biomacromolecules, 2002, 3(3): 511-516.

[22] Murphy W.L., Kohn D.H., Mooney D.J. Growth of Continuous Bonelike Mineral Within Porous Poly(lactide-co-glycolide) Scaffolds in vitro[J]. J. Biomed. Mater. Res., 2000, 50(1): 50-58.

[23] Teng S.H., Lee E.J., Wang P., . Three-layered Membranes of Collagen/hydroxyapatite and Chitosan for Guided Bone Regeneration[J]. J. Biomed. Mater. Res. B, Appl. Biomater., 2008, 87(1): 132-138.