Photocatalytic degradation of E.coli membrane cell in the presence of ZnO nanowires

Abstract

The photocatalytic degradation of E.coli membrane cell by ZnO nanowires was studied using field-emission scanning electron microscope(FE-SEM), fluorescence microscopy, and Attenuated total reflection fourier transform infrared(ATR-FTIR). The outer membrane of E.coli was removed completely in the presence of ZnO nanowires under UV irradiation, and the cells became twisted shapes without a mechanically strong network. After ZnO nanowires photocatalysis, the permeability of the treated cells increased to some degree that could be confirmed by quantum dots labeling technique. Structural changes in the cell wall membrane were revealed by the decay of the characteristic groups bands in ATR-FTIR spectra.

Keywords

photocatalysis / E.coli / ZnO / nanowire / permeability

References

[1] Lonnen J., Kilvington S., Kehoe S. C., . Solar and Photocatalytic Disinfection of Protozoan, Fungal and Bacterial Microbes in Drinking Water[J]. Water Research, 2005, 39(5): 877-883.

[2] Liu H., Yang T. C. K. Photocatalytic Inactivation of Escherichia coli and Lactobacillus Helveticus by ZnO and TiO₂ Activated with Ultraviolet Light[J]. Process Biochemistry, 2003, 39(4): 475-481.

[3] Chen F., Yang X., Mak H. K. C., . Photocatalytic Oxidation for Antimicrobial Control in Built Environment: A Brief literature Overview[J]. Building and Environment, 2010, 45(8): 1747-1754.

[4] Marugán J., van Grieken R., Pablos C., . Analogies and Differences between Photocatalytic Oxidation of Chemicals and Photocatalytic Inactivation of Microorganisms[J]. Water Research, 2010, 44(3): 789-796.

[5] Liu P., Liu Y., Lu Z., . Study on Biological Effect of La³⁺ on Escherichia Coli by Atomic Force Microscopy[J]. Journal of Inorganic Biochemistry, 2004, 98(1): 68-72.

[6] Kiwi J., Nadtochenko V. Evidence for the Mechanism of Photocatalytic Degradation of the Bacterial Wall Membrane at the TiO₂ Interface by ATR-FTIR and laser Kinetic Spectroscopy[J]. Langmuir, 2005, 21(10): 4631-4641.

[7] Nadtochenko V. A., Rincon A. G., Stanca S. E., . Dynamics of E-Coli Membrane Cell Peroxidation during TiO₂ Photocatalysis Studied by ATR-FTIR Spectroscopy and AFM Microscopy[J]. Journal of Photochemistry and Photobiology A-Chemistry, 2005, 169(2): 131-137.

[8] Li D., Haneda H. Morphologies of Zinc Oxide Particles and Their Effects on Photocatalysis[J]. Chemosphere, 2003, 51(2): 129-137.

[9] Chakrabarti S., Dutta B. K. Photocatalytic Degradation of Model Textile Dyes in Wastewater Using ZnO as Semiconductor catalyst[J]. Journal of Hazardous Materials, 2004, 112(3): 269-278.

[10] Zheng Y., Chen C., Zhan Y., . Luminescence and Photocatalytic Activity of ZnO Nanocrystals: Correlation Between Structure and Property[J]. Inorganic Chemistry, 2007, 46: 6675-6682.

[11] Yu J., Yu X. Hydrothermal Synthesis and Photocatalytic Activity of Zinc Oxide Hollow Spheres[J]. Environmental Science & Technology, 2008, 42(13): 4902-4907.

[12] Chu D., Masuda Y., Ohji T., . Formation and Photocatalytic Application of ZnO Nanotubes Using Aqueous Solution[J]. Langmuir, 2010, 26(4): 2811-2815.

[13] Hu H., Huang X., Deng C., . Hydrothermal Synthesis of ZnO Nanowires and Nanobelts on a Large scale[J]. Materials Chemistry and Physics, 2007, 106: 58-62.

[14] Liu P., Wang Q. S., Li X. Studies on CdSe/L-cysteine Quantum Dots Synthesized in Aqueous Solution for Biological Labeling[J]. Journal of Physical Chemistry C, 2009, 113(18): 7670-7676.