Extraction and characterization of PHB from Acidiphilium cryptum DX1-1

Abstract

A new bacterial strain, was designated as strain Acidiphilium cryptum DX1-1, accumulates intracellular poly-β-hydroxybutyrate particles, four methods which have advantages and disadvantages for each were employed to extract PHB. Chloroform-sodium hypochlorite method is the best in extracting PHB form Acidiphilium cryptum DX1-1. The extraction rate reaches 73%, the purification rate is 92% and molecular weight is 326 kg/mol. Then the PHB extracted by this method was analyzed by ultraviolet-visible absorption spectroscopy, fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR). The results show that PHB from strain DX1-1 has the same biochemical structure and character with PHB standard. Mass spectrometer (MS) analysis reveals that the long chain of PHB is destroyed when treated by chloroform-sodium hypochlorite. The differential scanning calorimetry (DSC) of PHB shows PHB from Acidiphilium cryptum DX1-1 has low degree of crystallinity which makes the PHB has a wider range of applications.

Keywords

Acidiphilium cryptum / PHB / extraction / characterization

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