Biocompatibility of FGL peptide self-assembly nanofibers with neural stem cells in vitro

Abstract

In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells (NSCs), FGL pepitide-amphiphile (FGL-PA) was synthesized by solid-phase peptide synthesis technique. The diluted hydrochloric acid was added into FGL-PA solution to reduce the PH value and accordingly induce self-assembly. The morphological features of the assembled material were studied by transmission electron microscope. NSCs were cultured and added with self-assembled FGL-PA. CCK-8 kit was used to test its effect on the proliferation of NSCs. The differentiation of NSCs was also tested after FGL-PA assembled material added. The experimental results showed that FGL-PA could be self-assembled to form a hydrogel. TEM analysis showed the self-assembled hydrogel was nanofibers with diameter of 10–20 nm and length of hundreds nanometers. FGL-PA with concentrations of 50,100, or 200 mg/L could promote the proliferation of NSCs, and absorbance of them was increased (P<0.05). The rate of neurons differentiated from NSCs was improved greatly by FGL-PA assembled material compared with control (P<0.05). The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel, which had good biocompatibility with NSCs.

Keywords

peptide / nanofiber / biomaterial / neural stem cell / biocompatibility

References

[1] Iwata A., Browne K.D., Pfister B.J., Gruner J.A., Smith D.H. Long-term Survival and Outgrowth of Mechanically Engineered Nervous Tissue Constructs Implanted into Spinal Cord Lesions[J]. Tissue Eng., 2006, 12(1): 101-110.

[2] Kiss J.Z., Muller D. Contribution of the Neural Cell Adhesion Molecule to Neuronal and Synaptic Plasticity[J]. Rev. Neurosci., 2001, 12(4): 297-310.

[3] Welzl H., Stork O. Cell Adhesion Molecules: Key Players in Memory Consolidation[J]. News Physiol. Sci., 2003, 18: 147-150.

[4] Kiselyov V.V., Soroka V., Berezin V., . Structural Biology of NCAM Homophilic Binding and Activation of FGFR[J]. J. Neurochem., 2005, 94(5): 1169-1179.

[5] Klementiev B., Novikova T., Novitskaya V., . A Neural Cell Adhesion Molecule-derived Peptide Reduces Neuropathological Signs and Cognitive Impairment Induced by Abeta25-35[J]. Neuroscience, 2007, 145(1): 209-224.

[6] Anderson A.A., Kendal C.E., Garcia-Maya M., . A Peptide from the First Fibronectin Domain of NCAM Acts as an Inverse Agonist and Stimulates FGF Receptor Activation, Neurite Outgrowth and Survival[J]. J. Neurochem., 2005, 95(2): 570-583.

[7] Galli R., Gritti A., Bonfanti L., . Neural Stem Cell: An Overview[J]. Circ. Res., 2003, 92(6): 596-608.

[8] Holmes T.C. Novel Peptide-based Biomaterial Scaffolds for Tissue Engineering[J]. Trends Biotechnol., 2002, 20(1): 16-21.

[9] Genove E., Shen C., Zhang S., . The Effect of Functionalized Self-assembling Peptide Scaffolds on Human Aortic Endothelial Cell Function[J]. Biomaterials, 2005, 26(16): 3341-3351.