Cytocompatibility of self-assembled hydrogel from IKVAV-containing peptide amphiphile with neural stem cells

Yulin Song; Qixin Zheng; Xiaodong Guo; Jianfeng Zheng

doi:10.1007/s11595-009-5753-6

Journal of Wuhan University of Technology Materials Science Edition ›› 2009, Vol. 24 ›› Issue (5) : 753 -756. DOI: 10.1007/s11595-009-5753-6

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Cytocompatibility of self-assembled hydrogel from IKVAV-containing peptide amphiphile with neural stem cells

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Abstract

Neural Stem Cells (NSCs) were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile (IKVAV-PA) for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional (3D) hydrogels (Experimental Group, EG) or surface of coverslips (Control Group, CG), double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG (P<0.05). The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.

Keywords

IKVAV / hydrogel / cytocompatibility / proliferation

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Yulin Song, Qixin Zheng, Xiaodong Guo, Jianfeng Zheng. Cytocompatibility of self-assembled hydrogel from IKVAV-containing peptide amphiphile with neural stem cells. Journal of Wuhan University of Technology Materials Science Edition, 2009, 24(5): 753-756 DOI:10.1007/s11595-009-5753-6

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