In vitro biomineralization of glutaraldehyde crosslinked chitosan/glutamic acid films

Abstract

In vitro biomineralization of glutaraldehyde crosslinked chitosan/glutamic acid films were studied. IR and ESCA (electron spectroscopy for chemical analysis) determinations confirm that chitosan and glutamic acid are successfully crosslinked by glutaraldehyde to form chitosan-glutamic acid surfaces. Composite films were soaked in saturated Ca(OH)₂ solution for 8 d and then immersed in simulated body fluid (SBF) for more than 20 d. Morphological characterizations and structure of calcium phosphate coatings deposited on the films were studied by SEM, XRD, and EDAX (energy dispersive X-ray analysis). Initially, the treatment in SBF results in the formation of single-layer calcium phosphate particles over the film surface. As immersion time increases, further nucleation and growth produce the simulated calcium-carbonate hydroxyapatite coating. ICP results show Ca/P ratio of calcium phosphate coating is a function of SBF immersion time. The inducing of glutamic acid improves the biomineralization property of chitosan films.

Keywords

chitosan / biomineralization / calcium phosphate / glutamic acid

References

[1] Beppu M. M., Aimoli C. G. Influence of Acetylation on in Vitro Chitosan Membrane Biomineralization[J]. Key Eng. Mater., 2004, 254-2: 311-314.

[2] Wan A. C. A., Khor E., Hastings G. W. Preparation of a Chitin-apatite Composite by in Situ Precipitation onto Porous Chitin Scaffolds[J]. J. Biomed. Mater. Res., 1998, 41: 541-548.

[3] Wan A. C. A., Khor E., Wong J. M., . Promotion of Calcification on Carboxymethylchitin Discs[J]. Biomaterials, 1996, 17: 1 529-1 534.

[4] Yokogawa Y., PazReyes J., Mucalo M. R., . Growth of Calcium Phosphate on Phosphorylated Chitin Fibers[J]. J. Mater. Sci. Mater. Med., 1997, 8: 407-412.

[5] Varma H. K., Yokogawa Y., Espinosa F. F., . Porous Calcium Phosphate Coating over Phosphorylated Chitosan Film by a Biomimetic Method[J]. Biomaterials, 1999, 20: 879-884.

[6] Beppu M. M., Santana C. C. In Vitro Biomineralization of Chitosan[J]. Key Eng. Mater., 2001, 192-1: 31-34.

[7] Kato T., Sugawara A., Hosoda N. Calcium Carbonate-organic Hybride Materials[J]. Advanced Materials, 2002, 14(12): 869-877.

[8] Muzzarelli C., Muzzarelli R. A. A. Natural and Artificial Chitosan-inorganic Composites[J]. J. Inorg. Biochem., 2002, 92: 89-94.

[9] Falini G., Albeck S., Weiner S., . Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules[J]. Science, 1996, 271: 67-69.

[10] Oyrton A. C., Monteiro J., Claudio A. Some Studies of Crosslinking Chitosan-glutaraldehyde Interaction in a Homogeneous System[J]. Int. J. Biol. Macromol., 1999, 26: 119-125.

[11] Zhang Y., Zhang M. Microstructurural and Mechanical Characterization of Chitosan Scaffolds Reinforced by Calcium Phosphates[J]. J. Non-crystal. Solids, 2001, 282: 159-164.

[12] Chung T. W., Lu Y. F., Wang S. S., . Growth of Human Endothelial Cells on Photochemically Grafted Gly-arg-gly-asp(GRGD) Chitosans[J]. Biomaterials, 2002, 23: 4 803-4 809.

[13] Calvert P. Biomimetic Mineralization: Processes and Prospects[J]. Mater. Sci. and Eng., 1994, 1: 69-74.

[14] Tretinnikov O. N., Kato K., Ikada Y. In Vitro Hydroxyapatite Deposition onto a Film Surface-grafted with Organophosphate Polymer[J]. J. Biomed Mater. Res., 1994, 28: 1 365-1 373.

[15] Abe Y., Kokubo T., Yamamura T. Apatite Coating on Ceramics, Metal and Polymers Utilizing a Biological Process[J]. J. Mater. Sci. Mater. Med., 1990, 1: 233-238.

[16] Dalas E., Kallitis J. K., Koutsoukos P. G. Crystallization of Hydroxyapatite on Polymers[J]. Langmuir, 1991, 7: 1 822-1 826.

[17] Viala S., Freche M., Lacout J. L. Effect of Chitosan on Octacalcium Phosphate Crystal Growth[J]. Carbohydr. Polym., 1996, 29: 197-201.

[18] Akazawa T., Kobayashi M., Kanno T. Characterization of Albumin- and Lysozyme-adsorption Evaluated on Two Differently Prepared Apatite[J]. J. Mater. Sci., 1998, 33: 1 927-1 931.

[19] Feng F., Liu Y., Zhao B. Y., . In Vitro Biomineralization of Glutaraldehyde Crosslinked Chitosan Films[J]. J. Wuhan Univ. of Technol., 2005, 20(2): 20-23.

[20] Andrew C. A. W., Khor E., Hastings G. W. The Influence of Anionic Chitin Derivatives on Calcium Phosphate Crystallization[J]. Biomaterials, 1998, 19: 1 309-1 316.