Influence of formate on bioactivity material-thuringiensin synthesized by Bacillus thuringiensis YBT-032

Abstract

The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α-ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g⁻¹·h⁻¹ and transformation ratio from glucose to thuringiensin increased by 43.30%.

Keywords

bacillus thuringiensis / precursor / formate / thuringiensin

References

[1] Gardner W. A. Enhanced Activity of Selected Combinations of Bacillus Thuringiensis and Beta-exotoxin Against Fall Armyworm (Lepidoptera: Noctuidae) Larvae[J]. J. Econ. Entomol., 1988, 81: 463-469.

[2] Devidas P., Rehberger L. A. The Effects of Exotoxin (Thuringiensin) from Bacillus Thuringiensis on Meloidogyne Incognita and Caenorhabditis Elegans[J]. Plant and Soil, 1992, 145: 115-120.

[3] Royalty R N, Hall F R, Taylor R A J. Effects of Thuringiensin on Tetranychus Urticae (Acari: Tetranychidae) Mortality, Fecundity, and Feeding[J]. J. Econ. Entomol., 1990(83): 792–798

[4] Yu Z.N. Yu Z.N. Metabolism. Bacillus thuringiensis[M], 1990. Beijing: Science Press. 97-103.

[5] Tzeng Y M, Young Y H. Production of Thuringiensin from Bacillus Thuringiensis Using a Net-draft-tube, Modified Airlift Reactor[J]. World J. Microb. Biot., 1996 (12):32–37

[6] Wu W T, Hsu Y L, Ko Y F, et al. Effect of Shear Stress on Cultivation of Bacillus Thuringiensis for Thuringiensin Production[J]. Appl. Microbiol. Biotechnol., 2002 (58):175–177

[7] Chen S, Hong J Y, Wu W T. Fed-batch Culture of Bacillus thuringiensis Based on Motile Intensity[J]. J. Ind. Microbiol. Biotechnol., 2003 (30): 677–681

[8] Shen T., Wang J. Y. Shen T., Wang J. Y. Nucleic Acid Metabolism. Biochemistry[M], 1998. Beijing: Higher Education Press. 197-319.

[9] Müller R H, Loffhagen N, Babel W. Rapid Extraction of (di)nucleotides from Bacterial Cells and Determination by Iion-pair Reversed-phase HPLC[J]. J. Microbiol. Methods, 1996 (25):29–35

[10] Henry L, George N B, Ka-yiu S. Metabolic Engineering of Aerobic Succinate Production Systems in Escherichia coli to Improve Process Productivity and Achieve the Maximum Theoretical Succinate Yield[J]. Metab. Eng., 2005(7): 116–127

[11] Hulya A K, Leman T. Vancomycin Antibiotic Production and TCA-glyoxalate Pathways Depending on the Glucose Concentration in Amycolatopsis Orientalis[J]. Enzyme Microb. Technol., 2006 (38):727–734

[12] Muriel C B, Armel G, Lindley N D. Growth Rate-dependent Modulation of Carbon Flux through Central Metabolism and the Kinetic Consequences for Glucose-limited Chemostat Cultures of Corynebacterium glutamicum[J]. Appl. Environ. Microbiol., 1996 (62):429–436.