Rheological and biological characteristics of hyaluronic acid derivative modified by polyethylene glycol

Abstract

Hyaluronic acid (HA) was chemically modified by polyethylene glycol. Meanwhile, the dynamic mechanics properties of HA derivative and its viscoelastic changes were measured on 3ARES3 Rheometer (Japan) at 25 °C. Dried cross-linked films of 10×10 mm² were immersed in phosphate buffered saline(PBS: pH 7.4) at 37 °C with different time periods to measure its water content and in vitro degradation. Moreover, cell cultured solutions, which were in the different cultivation vesse with 1 mg/mL Solution of HA derivative as doing experimental sample for 2 d, 4 d and 7 d, were observed, respectively, by an inverted discrepancy microscope. The cell relative growth rate was analyzed with the SPSS10.0 mathematic statistic software. Based on the above experiments, structure-modified HA derivative can meet the requirements of biomaterials in view of rheological and degradation in vitro and cytotoxicity charactereistics from clinical medical aspect under this experiment conditions.

Keywords

hyaluronic acid / viscoelasticity / water content / degradation / cytotoxicity

References

[1] Laurent T. C., Laurent U. B., Fraser J. R. The Structure and Function of Hyaluronan: An Overview[J]. Immunol Cell Biol., 1996, 74: A1-A5.

[2] Kim B. S., Mooney D. J. Development of Biocompatible Synthetic Extracellular Matrices for Tissue Engineering[J]. Trends Biotechnol., 1998, 16(5): 224-227.

[3] Chen J., Xu Z., Gu Q., . Research on Rheologyical Property of Hyaluronic Acid Derivative Cross-linked by Polyethylene Glycol[J]. J. of Tongji University, 2006, 34(1): 34-38.

[4] Ambrosio L. A., Borzacchiello P. A., Netti P. A., . Properties of New Materials: Rheological Study on Hyaluronic Acid and Its Derivative Solutions[J]. JMS-Pure and Applied Chemistry, 1999, A36(718): 991-995.

[5] Wik B. H., Wik O. Rheology of Hyaluronan and Its Derivative. The Chemistry, Biology and Medical Applications of Hyaluronan and Its Derivatives[M], 1998. London: Portland Press. 25-29.

[6] Balazs E. A. The Viscoelastic Intercellular Matrix and Control of Cell Function by Hyaluronan. The Chemistry, Biology and Medical Application of Hyaluronan and Its Derivatives[M], 1998. London: Portland Press. 185-188.

[7] Tomihata K., Ikada Y. Preparation of Cross-linked Hyaluronic Acid Films of Low Water Content[J]. J. Biomaterials, 1997, 18(3): 189-194.

[8] Tomihata K., Burczak K., Shiraki K., . Shalaby S. W., Ikada Y., Lannger R., Williams J., . Cross-linking and Biodegradation of Native and Denatured Collagen[C]. In Polymers of Biological and Biomedical Significance, 1994. Washington DC: American Chemical Society. 275-278.

[9] Kobayashi H., Shiraki K., Ikada Y. Toxicity Test of Biodegradable Polymers by Implantation in Rabbit Cornea[J]. J Biomed. Mater. Res., 1992, 26: 1 463-1 466.

[10] Li Y., Yang Z., Xie H., . Research on Cell Biocompatibility in Vitro of Bio-derivative Materials. Chinese J. Reparative and Reconstructive Surgery, 2001, 15(4): 227-231.

[11] Sung H.-w., Huang D.-n., Huang lynnl H., . In Vitro Evaluation of Cytotoxicity of a Naturally Ouurring Cross-linking Reagent for Biological Tissue Fixation[J]. J. Biomater. Sci. Polymer Edn., 1999, 10(1): 63-67.

[12] Lv X., Kappert H.F. New Method of Estimated Cytotocity of Dentistry Materials (MTT experiment)[J]. Chinese J. of Nonnasality Medicine, 1995, 30(5): 337-341.