Copper phthalocyanine catalysis to oxidation of adrenaline by oxygen and its application in adrenaline detection

Abstract

The oxidation of adrenaline by dioxygen using copper phthalocyanine (CuPc) as the catalyzer was studied. CuPc has the optimal catalytic pH of 8.0 and the optimal catalytic temperature of 55 °C. It also has good storage and operation stability. The fiber optic adrenaline biosensor based on CuPc catalysis and fluorescence quenching was fabricated and studied. This sensor has the detection range of 7.0×10⁻⁵–1.5×10⁻⁴ mol/L, the response time of 4 min, good reproducibility and stability.

Keywords

CuPc / catalytic oxidation / mimic enzyme / fiber optic adrenaline sensor

References

[1] Palop S. G., Romero A. M., Calatayud J. M. Oxidation of Adrenaline and Noradrenaline by Solved Molecular Oxygen in a FIA Assembly[J]. J. Pharm. Biomed. Anal., 2002, 27: 1 017-1 025.

[2] Nagaraja P., Vasantha R. A., Sunitha K. R. A New Sensitive and Selective Spectrophotometric Method for the Determination of Catechol Derivatives and Its Pharmaceutical Preparations[J]. J. Pharm. Biomed. Anal., 2001, 25: 417-424.

[3] Yao H., Sun Y. Y., Lin X. H., . Flow-injection Chemiluminescence Determination of Catecholamines Based on Their Enhancing Effects on the Luminol-potassium Periodate System[J]. Luminescence, 2006, 21(2): 112-120.

[4] Loay K. A., Anas M. A., Mayada H. A. Flow Injection-spectrophotometeric Determination of Some Catecholamine Drugs in Pharmaceutical Preparations via Oxidative Coupling Reaction with p-toluidine and Sodium Periodate[J]. Analytica Chimica Acta, 2005, 538: 331-335.

[5] Zhou T. S., Hu Q., Yu H., . Separation and Determination of β-agonists in Serum by Capillary Zone Electrophoresis with Amperometric Detection[J]. Analytica Chimica Acta, 2001, 441: 23-28.

[6] Kasuga K., Tsutsui M. Some New Developments in the Chemistry of Metallophthalocyanines[J]. Coord chem.Rev., 1980, 32: 67-95.

[7] He X. C., Li P., Mo J. Y. Simultaneous Determination of Dopamine and Epinephrine at Modified Electrode by Derivative Voltammetry[J]. J. Instrum. Anal., 2000, 19: 19-22.

[8] Palop S. G., Romero A. M., Calatayud J. M. Oxidation of Adrenaline and Noradrenaline by Solved Molecular Oxygen in a FIA Assembly[J]. J. Pharm. Biomed. Anal., 2002, 27: 1 017-1 025.

[9] Zhang J. B., Chen X., Huang J., . Optical Fiber Oxygen Sensor for Detecting Dissolved Oxygen in Water[J]. J. Transducer Technology, 2002, 21: 4-7.

[10] Kitamura Y., Takatsuki T., Kawamoto M. Catalytic Activity of Silica Gels Bound Manganese(III)-Prophyrin on Oxidative Reaction of Adrenaline[J]. Chem. Pharm. Bull., 2007, 55(3): 500-506.

[11] Yang H. S., Peng T. Z., Shen B. E. Thin-layer Spectroelectrochemical Investigation of Dopamine Oxidation on Carbon Fiber Electrodes[J]. Chinese J of Zhejiang University, 2001, 28(3): 294-298.

[12] Chen B., Yang S. Q., Zhao C. D. Study on the Mechanisms of Catalytic Desulfurization With Binuclear Metallo Phthalocyanine(III)—The Electronic Structure of M (II) Pc-PcM (II) and Mode of Binding Between M (II) and Pc[J]. Chinese J. Mol. Sci., 1996, 12(3): 204-210.